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公开(公告)号：KR1020150047670A
公开(公告)日：2015-05-06
申请号：KR1020130126776
申请日：2013-10-23
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/85 , C12N15/63 , C12N15/8509
Abstract: 본발명은돼지의유청단백질유전자프로모터를이용한유선발현시스템에관한것으로서, 보다상세하게는돼지유청단백질유전자, 돼지유청단백질유전자프로모터, 이를포함하는발현벡터, 및이를이용한목적단백질의제조방법에관한것이다. 돼지유청단백질유전자프로모터는목적단백질의유선특이적발현을촉진하므로, 본발명의프로모터, 및이를이용한발현벡터에의해형질전환된동물은유즙중에목적단백질을고농도로분비하게되어의약학적으로중요한가치를갖는유용단백질의생산분야에유용하게사용될수 있다.
Abstract translation: 本发明涉及使用猪中乳清蛋白的基因启动子的乳腺中的表达系统，更具体地，涉及猪的乳清蛋白基因，猪的乳清蛋白的基因启动子，含有该乳酸蛋白的表达载体，以及 使用其的靶蛋白的制造方法。 猪中乳清蛋白的基因启动子在乳腺中促进靶蛋白的表达，并且因此转化的动物由启动子和表达载体使用相同的分泌高浓度的目标蛋白在牛奶中。 因此，该系统可用于有用蛋白质的生产领域，作为医药领域的重要价值。
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2.发明公开단계별 면역거부반응 억제 다중유전자가 α-Ｇａｌ 유전자에 적중된 복합형질전환 세포주 및 그의 제조방법 审中-实审通过α-GAL基因靶向插入载体表达免疫抑制基因的多重转化细胞系及其制备方法
公开(公告)号：KR1020150008719A
公开(公告)日：2015-01-23
申请号：KR1020130083027
申请日：2013-07-15
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
IPC: C12N15/63 , C12N15/85 , A01K67/027
CPC classification number: C12N15/63 , A01K67/027 , C12N15/64 , C12N15/74 , C12N15/85
Abstract: The present invention relates to: a multi-transgenic cell line that is transformed by a multi-transgenic vector and includes stepwise immunological rejection inhibitory genes knocked in a α-1,3-galactosyltransferase gene; and a method for preparing the same cell line. The stepwise immunological rejection can occur when a hetero-organ is transplanted. According to the present invention, the cell line transformed with multiple genes capable of adjusting the stepwise immunological rejection at once is identified. Therefore, the cell line can be advantageously used to efficiency produce various artificial organs and to produce cloned pigs, which are multiply transformed.
Abstract translation: 本发明涉及：多转基因载体转化的多转基因细胞系，包括敲入α-1,3-半乳糖基转移酶基因的逐步免疫排斥抑制基因; 以及制备相同细胞系的方法。 异种器官被移植时可能发生逐步的免疫排斥反应。 根据本发明，鉴定了能够同时调整逐步免疫排斥的多个基因转化的细胞系。 因此，细胞系可以有利地用于效率地产生各种人造器官并产生多重转化的克隆猪。
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公开(公告)号：KR101444297B1
公开(公告)日：2014-10-15
申请号：KR1020120001427
申请日：2012-01-05
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/85 , C12N15/113 , A01K67/027
Abstract: 본 발명은 CYP4F16 유전자가 넉다운된 형질전환마우스 및 그 제조방법에 관한 것이다. 보다 상세하게는, 수면병에 대한 저항성을 나타내는 CYP4F16유전자가 넉다운된 형질전환마우스 및 그 제조방법에 관한 것이다. 본 발명의 형질전환 마우스를 이용하면, 트리파노솜의 체내 침입후 발생되는 면역관련 유전자 중 수면병의 발생생원인 규명하고, 이를 치료할 수 있는 신약을 개발하는 데 이용될 수 있다.
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公开(公告)号：KR101435635B1
公开(公告)日：2014-08-29
申请号：KR1020120108343
申请日：2012-09-27
Applicant: 전남대학교산학협력단 , 대한민국(농촌진흥청장)
IPC: C12N15/85 , C12N15/54 , A01K67/027
Abstract: 알파 1,3-갈락토실트랜스퍼라아제(α1,3-galactosyl transferase, α1,3-GT) 유전자의 위치에 보체 조절 단백질(decay accelerating factor, DAF) 유전자와 인간의 알파 1,2-푸코실트랜스퍼라아제(human α1,2-fucosyltransferase, HT) 유전자를 적중시킬 수 있는 넉인 벡터, 상기 벡터가 도입된 세포, 상기 세포로 핵이식된 수정란, 상기 벡터에 의해 넉 아웃된 형질전환 동물, 및 이의 제조방법에 관한 것이다. 본 발명의 넉인 벡터는 이종장기이식에서 발생하는 초급성 거부반응이 억제된 형질전환 동물의 제조에 이용될 수 있으므로, 보다 효율적인 이종장기이식에 사용될 수 있는 형질전환 동물의 생산에 널리 활용될 수 있을 것이다.
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公开(公告)号：KR101429225B1
公开(公告)日：2014-08-14
申请号：KR1020120049173
申请日：2012-05-09
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 한우의 수정적기를 측정하기 위한 조성물 및 이를 이용한 한우 수정적기의 측정방법에 관한 것으로서, 보다 상세하게는 한우 발정 시기에 특이적으로 발현되는 단백질을 이용한 수정적기 측정용 조성물 및 이를 이용한 측정방법에 관한 것이다. 본 발명에 따르면, 인공수정을 통해 송아지를 생산하는데 있어서 가장 중요시되는 발정과 수정을 파악하는 과정에 있어서 보다 신뢰도 있고 과학적인 수정적기의 진단 키트 및 방법이 제공될 수 있다.
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Patent Agency Ranking
公开(公告)号：KR1020130080572A
公开(公告)日：2013-07-15
申请号：KR1020120001427
申请日：2012-01-05
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/85 , C12N15/113 , A01K67/027
Abstract: PURPOSE: A transgenic mouse in which CYP4F16 gene is knocked-down and a manufacturing method thereof are provided to inquire a cause of occurrence of a sleeping disease among immune-related genes generated after infestation of trypanosome and to develop new medicine for curing the diseases. CONSTITUTION: A siRNA expression recombinant vector for mouse CYP4F16 knocked-down is identified by a promoter and SEQ ID No. 1 that is operably connected to the promoter. The promoter is a U6 promoter. The recombinant vector is introduced to a transgenic mouse. A manufacturing method of a transgenic mouse in which CYP4F16 gene expression is knocked-down comprises the following steps of: (a) manufacturing a siRNA expression cassette for expressing CYP4F16 that is represented by a promoter and SEQ ID No. 1 that is operably connected to the promoter; (b) introducing the recombinant fused gene to a fertilized egg of a mouse; and (c) producing breeders by implanting the fertilized egg into a surrogate mother.
Abstract translation: 目的：提供CYP4F16基因被敲除的转基因小鼠及其制造方法，用于询问在锥虫感染后产生的免疫相关基因中发生睡眠疾病的原因，并开发用于治疗疾病的新药。 构成：用于小鼠CYP4F16敲除的siRNA表达重组载体通过与启动子可操作连接的启动子和SEQ ID No.1来鉴定。 启动子是U6启动子。 将重组载体导入转基因小鼠。 其中CYP4F16基因表达被敲除的转基因小鼠的制造方法包括以下步骤：（a）制备用于表达CYP4F16的siRNA表达盒，所述siRNA表达盒由启动子和SEQ ID No.1表示，所述启动子和SEQ ID No.1可操作地连接到 启动子; （b）将重组融合基因导入小鼠受精卵; 和（c）通过将受精卵植入替代母亲来培育育种者。
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公开(公告)号：KR100375672B1
公开(公告)日：2003-03-15
申请号：KR1019990032079
申请日：1999-08-05
Applicant: 대한민국(농촌진흥청장)
IPC: C07K14/59
Abstract: PURPOSE: Provided is a method for manufacturing a single chain bovine Follicle-stimulating hormone(FSH) which shows a stronger hormone activity than one of natural hormones, so as to be used for animal drugs. Thereby, FSH is easily manufactured in a high yield as forming FSH consisting of alpha and beta short fragments, into single chain FSH. CONSTITUTION: A method for manufacturing a single chain bovine Follicle-stimulating hormone(FSH) is characterized by the following steps of: i) performing polymerase chain reaction to link DNA coding an alpha short fraction of bovine FSH to DNA coding a beta short fragment of the hormone, using 4kinds of oligonucleic acids bases as primers, to obtain cDNAs of the alpha and beta fractions and cloning the cDNAs with pUC119; ii) multiplying cloned DNA through PCR to obtain bFSH composed of cDNA of alpha and beta fractions, cutting the bFSH by restriction enzyme Kpnl/Xbal and linking the bFSH to vector pUC119 to form expression vector pcDAN3-bFSH; iii) inserting the expression vector pcDAN3-bFSH into CHO-K1 and cultivating CHO-K1 in a medium Ham F-12 including penicillin, streptomycin, glutamine, 10FCS and G418, in the presence of 5CO2 and air, at 37 deg.C for 2 weeks and selecting stable cells: iv) cultivating selected cells at 20 ml of CHO-S-SFM-11 medium containing 50 units/ml of penicillin and 50 microg/ml of streptomycin at 37 deg.C for 48 hours, and centrifuging supernatant liquid obtained through the cultivation for 60 minutes to remove cell remnants.
Abstract translation: 目的：提供一种生产单一链牛卵泡刺激素（FSH）的方法，其表现出比天然激素更强的激素活性，以用于动物药物。 因此，FSH很容易以高产率生产，形成由α和β短片段组成的FSH，进入单链FSH。 构成：制造单链牛卵泡刺激素（FSH）的方法的特征在于以下步骤：i）进行聚合酶链式反应以将编码牛FSH的α短部分的DNA与编码β短片段的DNA 使用4种寡核苷酸碱基作为引物，获得α和β部分的cDNA并用pUC119克隆cDNA; ii）通过PCR扩增克隆的DNA以获得由α和β部分的cDNA组成的bFSH，通过限制性内切酶KpnI / XbaI切割bFSH并将bFSH与载体pUC119连接以形成表达载体pcDAN3-bFSH; iii）将表达载体pcDAN3-bFSH插入到CHO-K1中，并且在37℃下在含有青霉素，链霉素，谷氨酰胺，10FCS和G418的培养基Ham F-12中，在5CO2和空气存在下培养CHO-K1 2周并选择稳定的细胞：iv）在20ml包含50单位/ ml青霉素和50μg/ ml链霉素的CHO-S-SFM-11培养基中于37℃培养选择的细胞48小时，并离心上清液 通过培养60分钟获得的液体除去细胞残留物。
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公开(公告)号：KR1020010081456A
公开(公告)日：2001-08-29
申请号：KR1020000006888
申请日：2000-02-14
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/00
CPC classification number: A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/108 , A01K2267/01 , C12N15/8509
Abstract: PURPOSE: A method for producing transgenic porcines for producing human erythropoietin(EPO) is provided, thereby human erythropoietin(EPO) can be mass-produced. CONSTITUTION: The method for producing transgenic porcines comprising the steps of: cloning the genomic DNA of human erythropoietin(EPO) using a WAP promoter isolated from the mammary gland of mouse by polymerase chain reaction(PCR); preparing the EPO expression vector containing human erythropoietin(hEPO) and SV40 poly A gene; injecting eCG hormone and hCG hormone into the fertilized eggs of porcine to induce overovulation; recovering the fertilized eggs and inserting the above DNA into a male cell nucleus using a manipulator; transplanting the cell into a surrogate porcine mother; and producing transgenic porcines having DNA represented by SEQ ID NO: 1 from the surrogate porcine mother.
Abstract translation: 目的：提供生产人促红细胞生成素（EPO）的转基因猪的方法，从而大量生产人促红细胞生成素（EPO）。 构成：生产转基因猪的方法，包括以下步骤：使用通过聚合酶链反应（PCR）从小鼠乳腺分离的WAP启动子克隆人促红细胞生成素（EPO）的基因组DNA; 制备含有促红细胞生成素（hEPO）和SV40聚A基因的EPO表达载体; 将eCG激素和hCG激素注射到猪的受精卵中以诱导过度排卵; 回收受精卵，并使用机械手将上述DNA插入雄性细胞核; 将细胞移植到替代猪母亲中; 并从代孕猪母体生产具有由SEQ ID NO：1表示的DNA的转基因猪。
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公开(公告)号：KR100271853B1
公开(公告)日：2000-11-15
申请号：KR1019970067574
申请日：1997-12-10
Applicant: 대한민국(농촌진흥청장)
IPC: A61D1/00
Abstract: PURPOSE: An operating table for pig comprising an operation supporting plate capable of going up and down by oil pressure and being easily moved by a tread board, and a moving plate for effectively fixing pigs is provided, which enables an operator to conveniently operate regardless of the physical condition of the operator. CONSTITUTION: In an operating table for pig capable of moving up and down by a piston of a hydraulic cylinder, this table comprises an operation supporting plate(10) comprising a fixed supporting plate(11) in its center and a moving supporting plate(12)(12') on both sides; a moving pipe mounted at the lower part of the fixed supporting plate to support and connected to a pipe; a screw handle(51) placed at one end part of the supporting plate(30); a guide hole(31) placed in the center of the supporting plate; and two link members(40)(40').
Abstract translation: 目的：提供一种用于猪的操作台，其包括能够通过油压上下运动并容易被踏板移动的操作支撑板，以及用于有效地固定猪的移动板，这使得操作者能够方便地操作，而不管 操作员的身体状况。 构成：在能够通过液压缸的活塞上下移动的猪的操作台中，该工作台包括操作支撑板（10），其包括在其中心的固定支撑板（11）和移动支撑板（12） ）（12'） 安装在固定支撑板的下部以支撑并连接到管道的移动管; 设置在支撑板（30）的一个端部处的螺丝把手（51）; 布置在所述支撑板的中心的引导孔（31） 和两个连杆构件（40）（40'）。
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公开(公告)号：KR100246528B1
公开(公告)日：2000-04-01
申请号：KR1019970051019
申请日：1997-10-02
Applicant: 대한민국(농촌진흥청장) , 축산업협동조합중앙회
IPC: G01N33/50
Abstract: 본 발명은 종모우 정액의 수정능 예측방법에 관한 것으로서, (a) 동결된 소정액을 융해하여 정자를 준비하는 단계와; (b) 준비된 HCB(heparin coated bead)와 상기 정자를 공배양하는 단계와; (c) 상기 공배양된 HCB와 정자에 있어, 상기 HCB 1개당 결합된 정자의 수를 관찰한 정자결합능(정자수/HCB)을 계산하는 단계를 포함하는 종모우 정액의 수정능 예측방법을 제공함으로써, 종모우 정자의 수정능력을 예측하여 고수태성 종모우의 선택적 이용을 도모함으로써 수태율 향상과 개량효율을 증진할 수 있는 특징이 있다.
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