누에 실샘 초미세분말유래 수용성 세리신을 포함하는 화장료 조성물
    3.
    发明公开
    누에 실샘 초미세분말유래 수용성 세리신을 포함하는 화장료 조성물 有权
    化妆品组合物,包括水溶性丝氨酸从丝光粉

    公开(公告)号:KR1020130030968A

    公开(公告)日:2013-03-28

    申请号:KR1020110094598

    申请日:2011-09-20

    CPC classification number: A61K8/987 A61K8/02 A61K8/64 A61Q19/00

    Abstract: PURPOSE: A cosmetic composition using water soluble sericin which is derived from silkworm grand ultrafine powder is provided to ensure excellent antioxidation, skin cell proliferation ability, and skin affinity. CONSTITUTION: A cosmetic composition contains 0.001-50 wt% of water soluble sericin which is derived from silkworm grand ultrafine powder. A method for preparing sericin comprises: a step of freeze-drying silkworm and isolating silkworm grand; a step of dissolving the silkworm grand in water and centrifuging to collect a supernatant; a step of adding alkali to the supernatant and hydrolyzing; and a step of neutralizing and desalting.

    Abstract translation: 目的:提供使用由蚕丝超细粉衍生的水溶性丝胶蛋白的化妆品组合物,以确保优异的抗氧化,皮肤细胞增殖能力和皮肤亲和力。 构成:化妆品组合物含有0.001-50重量%的来自蚕质超细粉末的水溶性丝胶蛋白。 一种制备丝胶蛋白的方法,包括:将蚕冷冻干燥并隔离蚕茧的步骤; 将蚕桑溶解在水中并离心收集上清液的步骤; 向上清液中加入碱水解水解步骤; 和中和和脱盐的一步。

    누에 실샘 유래 가수분해물을 포함하는 화장료 조성물
    5.
    发明公开
    누에 실샘 유래 가수분해물을 포함하는 화장료 조성물 有权
    化妆品组合物,包括从SilkWorm GLAND的水解产物

    公开(公告)号:KR1020140039370A

    公开(公告)日:2014-04-02

    申请号:KR1020120104477

    申请日:2012-09-20

    CPC classification number: A61K8/987 A61K8/64 A61K2800/80 A61Q19/00

    Abstract: The present invention relates to a cosmetic composition including a hydrolysate from a silkworm silk gland. A cosmetic composition of the present invention is characterized in including a hydrolysate from a silkworm silk gland, and more specifically, the hydrolysate is prepared by separating a gland from a silkworm, dissolving the separated gland into water, centrifuging and obtaining a supernatant, adding an alkaline compound to conduct hydrolysis, neutralizing and desalting. According to the present invention, provided is a cosmetic composition comprising a hydrolysate from a silkworm silk gland which is harmless to humans and having good antioxidant activity, dermal fibroblast proliferating properties, skin affinity, etc.

    Abstract translation: 本发明涉及包含来自蚕丝腺的水解产物的化妆品组合物。 本发明的化妆品组合物的特征在于包括来自蚕丝腺的水解产物,更具体地说,通过从蚕分离腺体,将分离的腺液溶解在水中,离心并得到上清液,加入 碱性化合物进行水解,中和脱盐。 根据本发明,提供了一种化妆品组合物,其包含来自对人类无害且具有良好抗氧化活性,真皮成纤维细胞增殖性质,皮肤亲和性等的蚕丝腺的水解产物。

    누에 혈림프 유래 단백질의 대량 정제방법 및 이를 통해 정제된 단백질
    6.
    发明授权
    누에 혈림프 유래 단백질의 대량 정제방법 및 이를 통해 정제된 단백질 有权
    使用丝朊蛋白和纯化蛋白质的蛋白质的质量纯化方法

    公开(公告)号:KR101346057B1

    公开(公告)日:2013-12-31

    申请号:KR1020120068585

    申请日:2012-06-26

    Abstract: The present invention relates to a mass purification method of a silkworm hemolymph-derived protein and a protein purified thereby. The mass purification method according to the present invention comprises a first step of freeze-drying silkworm to prepare a silkworm dried material; a second step of removing silk gland and obtaining the remainder hemolymph; a third step of crushing the hemolymph and adding water thereto to prepare a hemolymph lysate; a fourth step of subjecting the hemolymph lysate to centrifugation to obtain a supernatant; and a fifth step of subjecting the supernatant to column chromatography to isolate a protein of 70kDa. According to the present invention, the silkworm hemolymph is applicable for several uses, such as a cell cryopreservative, a medium supplement for cell culture, and the like. Especially, the ingredients remaining after the isolation of silkworm hemolymph are also reusable, so that the present invention contributes to an increase in the income of sericulture farmers. [Reference numerals] (AA) Silk gland removal;(BB) Grown-up silkworm;(CC) live body weight 3.13g;(DD) Centrifugation;(EE) Quick freezing;(FF) Grown-up silkworm freeze-drying 590 mg;(GG) pulverization/selection;(HH) hemolymph 380 mg;(II) Dissolution (distilled water);(JJ) 63 mg after heat treatment;(KK) 30k 70k separation;(LL) Final retrieval amount

    Abstract translation: 本发明涉及一种蚕血淋巴衍生蛋白的质量纯化方法和由此纯化的蛋白质。 根据本发明的质量净化方法包括:冷冻干燥蚕的第一步骤,制备蚕干物质; 去除丝腺并获得剩余血淋巴的第二步; 破碎血淋巴并向其中加入水以制备血淋巴溶胞产物的第三步骤; 第四步,使血淋巴溶胞产物离心得到上清液; 和将上清液进行柱色谱分离70kDa的蛋白质的第五步骤。 根据本发明,蚕血淋巴可应用于细胞冷冻保存剂,细胞培养用培养基等的多种用途。 特别是在分离蚕血淋淋后残留的成分也是可重复利用的,因此本发明有助于增加蚕食农民的收入。 (参考号)(AA)丝腺去除;(BB)成熟蚕;(CC)活体重3.13g;(DD)离心;(EE)快速冷冻;(FF)成熟蚕冷冻590 (GG)粉碎/选择;(HH)血淋巴380mg;(II)溶解(蒸馏水);(JJ)热处理后63mg;(KK)30k 70k分离;(LL)最终检索量

    누에 유래 혈림프를 포함하는 화장료 조성물
    7.
    发明公开
    누에 유래 혈림프를 포함하는 화장료 조성물 有权
    化妆品组合物,其中包括来自丝光乳杆菌的HEMOLYMPH

    公开(公告)号:KR1020140062564A

    公开(公告)日:2014-05-26

    申请号:KR1020120127680

    申请日:2012-11-12

    CPC classification number: A61K8/987 A61K8/02 A61K2800/80 A61Q19/02

    Abstract: The present invention relates to a cosmetic composition including hemolymph derived from silkworms. The cosmetic composition of the present invention comprises hemolymph derived from silkworms, and more specifically, comprises hemolymph from silkworms obtained by putting a crude extract containing silkworm tissues remained after removing silk glands from the silkworms, treating with heat, and refining a supernatant solution obtained by continuous centrifugation. According to the present invention, the cosmetic composition comprising hemolymph derived from silkworms which is harmless to human body and has excellent anti-oxidant activity, anti-microbial activity, whitening effects, etc. is provided.

    Abstract translation: 本发明涉及包含从蚕衍生的血淋巴的化妆品组合物。 本发明的化妆品组合物包括来自蚕的血淋巴,更具体地,包括通过将从蚕中除去丝腺后残留的蚕组织的粗提取物,通过加热处理而得到的来自蚕的血淋巴,并且精制上清液 连续离心。 根据本发明,提供了包含对人体无害的具有优异的抗氧化活性,抗微生物活性,美白效果等的来源于蚕的血淋巴的化妆品组合物。

    누에 실샘 유래 가수분해물의 수득방법
    8.
    发明公开
    누에 실샘 유래 가수분해물의 수득방법 有权
    来自丝M兰的水解产物的获得方法

    公开(公告)号:KR1020130031194A

    公开(公告)日:2013-03-28

    申请号:KR1020120068600

    申请日:2012-06-26

    Abstract: PURPOSE: A method for preparing a silkworm grand-derived hydrolysate is provided to obtain a hydrolysate with excellent cell proliferation effect without a harmful solvent. CONSTITUTION: A method for preparing a silkworm grand-derived hydrolysate comprises: a step of preparing a silkworm; a step of freeze-drying the silkworm and isolating a silk gland; a step of dissolving the silk gland with water and preparing a first lysate; a step of centrifuging the first lysate and collecting supernatant; a step of adding alkali to the supernatant and performing hydrolysate to prepare a second lysate; a step of adding acid to the second lysate and neutralizing to prepare a neutralized solution; a step of desalting the neutralized solution and preparing a hydrolysate; and a step of freeze-drying the hydrolysate. [Reference numerals] (AA) Silkworm; (BB) Freeze-drying and isolating; (CC) Silk gland; (DD) Pulverizing; (EE) Powder; (FF) Dissolving in water; (GG) Centrifuging and isolating supernatant; (HH) Adding alkali; (II) Hydrolyzing; (JJ) Neutralizing; (KK) Desalting; (LL) Freeze-drying; (MM) Hydrolysate

    Abstract translation: 目的:提供一种制备蚕大分子水解物的方法,以获得没有有害溶剂的具有优异细胞增殖作用的水解产物。 构成:一种蚕源水解产物的制备方法,包括:制备蚕的步骤; 冷冻干燥蚕丝并分离丝腺的步骤; 将丝腺溶解在水中并制备第一裂解液的步骤; 离心第一裂解物并收集上清液的步骤; 向上清液中加入碱,并进行水解产物制备第二裂解液的步骤; 将酸添加到第二裂解物中并中和以制备中和溶液的步骤; 将中和溶液脱盐并制备水解产物的步骤; 和冷冻干燥水解物的步骤。 (附图标记)(AA)蚕; (BB)冷冻干燥和隔离; (CC)丝腺; (DD)粉碎; (EE)粉末; (FF)溶于水; (GG)离心分离上清液; (HH)加碱; (二)水解; (JJ)中和; (KK)脱盐; (LL)冷冻干燥; (MM)水解产物

    누에 실샘 유래 가수분해물의 수득방법
    10.
    发明授权
    누에 실샘 유래 가수분해물의 수득방법 有权
    来自丝M兰的水解产物的获得方法

    公开(公告)号:KR101346059B1

    公开(公告)日:2013-12-31

    申请号:KR1020120068600

    申请日:2012-06-26

    Abstract: 본 발명은 누에 실샘 유래 가수분해물의 수득방법에 관한 것이다.
    본 발명의 누에 실샘 유래 가수분해물의 수득방법은, 누에를 준비하는 제1단계, 상기 준비된 누에를 동결건조하여 실샘을 분리하고, 분리된 실샘을 물로 용해하여 제1 용해물을 제조하는 제2단계, 상기 제1 용해물을 원심분리한 후 상등액을 취하는 제3단계, 상기 상등액에 알칼리를 첨가한 후 가수분해하여 제2 용해물을 제조하는 제4단계, 상기 제2 용해물에 산을 넣고 중화하여 중화액을 제조하는 제5단계, 상기 중화액을 탈염하여 가수분해물을 수득하는 제6단계 및, 상기 수득된 가수분해물을 동결건조하는 제7단계를 포함하여 구성된다.
    본 발명에 의해, 유해한 용매를 사용하지 않으면서, 상온추출로 누에 실샘 유래 가수분해물의 수득이 가능하면서, 세포증식효과가 우수한 누에 실샘 유래 가수분해물의 수득방법이 제공된다.

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