公开(公告)号：KR100331170B1

公开(公告)日：2002-04-06

申请号：KR1019990049009

申请日：1999-11-05

Applicant: 대한민국(농촌진흥청장)

Inventor： 한봉희 ,  예병우 ,  구대회 ,  손성호

IPC: C12M3/00

Abstract: 본발명은나리(백합)의종묘생산에있어재료배양시사용되는생물반응기(bioreactor) 및이를이용한나리의자구비대촉진방법에관한것으로서, 조직배양을이용한나리종구대량번식에있어최종생산되는자구의크기를종래기술에비해증가시킬수 있게되어양구기간을단축하고종구의생산단가를저감시킬수 있도록하는데목적이있다. 그리고, 다른목적으로는, 식물체를간편하게주입할수 있고, 오염또한방지할수 있는생물반응기를제공하는데있다. 이를실현하기위한본 발명의자구생산방법은, 고체배지를이용하여인편, 줄기등조직에서저반부조직을유기하는단계; 상기형성된저반부를절단, 계대배양하여증식시키는단계; 및상기증식된저반부절편체를 2구생물반응기내에서액체배지에의해배양하는단계;를포함하게된다.

公开(公告)号：KR1020010045663A

公开(公告)日：2001-06-05

申请号：KR1019990049009

申请日：1999-11-05

Applicant: 대한민국(농촌진흥청장)

Inventor： 한봉희 ,  예병우 ,  구대회 ,  손성호

IPC: C12M3/00

Abstract: PURPOSE: A method for enlarging the size of lily bulblet with low cost is provided, which has no effect on the growth of the plant and causes no environmental PROBLEM. CONSTITUTION: The method for enlarging the size of lily bulblet is characterized by the following steps of: i) growing ramentum and stem in medium; ii) cutting basal plate and multiplying the basal plate through subculture; iii) moving segments of multiplied basal plate to a bioreactor; and iv) cultivating the segments in liquid medium of the bioreactor. Wherein the liquid medium is MS (Murashige Skoog) medium containing 1g/L of activated carbon to absorb secretions of the plant and sucrose and is replaced every eight weeks.

Abstract translation: 目的：提供一种以低成本扩大百合花粉大小的方法，对植物生长无影响，无环境问题。 构成：扩大百合大小的大小的方法的特点是：i）培养基中生长的茎和茎; ii）切割基底板并通过传代培养扩大基底板; iii）将增加的基板的段移动到生物反应器; 和iv）在生物反应器的液体培养基中培养细分。 其中液体培养基是含有1g / L活性炭的MS（Murashige Skoog）培养基，以吸收植物和蔗糖的分泌物并且每八周更换一次。