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公开(公告)号:KR100453568B1
公开(公告)日:2004-10-20
申请号:KR1020010034003
申请日:2001-06-15
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: PURPOSE: Provided are a microsatellite DNA marker combination for classification of rice varieties and specific code numbers given thereby, therefore each of 148 kinds of Korean rice has a specific code number, so that the Korean rice can be easily classified. CONSTITUTION: The microsatellite DNA marker combination for classification of rice varieties consists of six(6) microsatellite DNA markers of RM48, RM249, RM209, RM70, RM257 and OSR20, wherein they are positioned at the 2nd, 5th, 6th, 7th, 9th and 12th sites in a rice chromosome, respectively, and have their own numbers of two digits according to each PCR amplified allele. Each variety of the rice is given code number of 11 digits by combination of the 6 microsatellite markers according to chromosome numbers; and the microsatellite DNA markers are selected by using PCR amplification and 4% acrylamide electrophoresis.
Abstract translation: 目的:提供了用于水稻品种分类的微卫星DNA标记组合和由此给出的具体代码编号,因此148种韩国稻米中的每种具有特定的代码编号,从而韩国稻米可以容易地分类。 构成:用于水稻品种分类的微卫星DNA标记组合由RM48,RM249,RM209,RM70,RM257和OSR20六个(6)微卫星DNA标记组成,分别位于第2,5,6,7,9和 根据每个PCR扩增的等位基因分别具有它们自己的两位数。 根据染色体数目,通过6个微卫星标记的组合给每个品种的11个数字编码; 通过PCR扩增和4%丙烯酰胺电泳筛选微卫星DNA标记。
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公开(公告)号:KR1020020095794A
公开(公告)日:2002-12-28
申请号:KR1020010034003
申请日:2001-06-15
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: PURPOSE: Provided are a microsatellite DNA marker combination for classification of rice varieties and specific code numbers given thereby, therefore each of 148 kinds of Korean rice has a specific code number, so that the Korean rice can be easily classified. CONSTITUTION: The microsatellite DNA marker combination for classification of rice varieties consists of six(6) microsatellite DNA markers of RM48, RM249, RM209, RM70, RM257 and OSR20, wherein they are positioned at the 2nd, 5th, 6th, 7th, 9th and 12th sites in a rice chromosome, respectively, and have their own numbers of two digits according to each PCR amplified allele. Each variety of the rice is given code number of 11 digits by combination of the 6 microsatellite markers according to chromosome numbers; and the microsatellite DNA markers are selected by using PCR amplification and 4% acrylamide electrophoresis.
Abstract translation: 目的:提供一种用于水稻品种分类的微卫星DNA标记组合和由此给出的具体代码,因此,每种148种韩国大米具有特定的编号,从而可以容易地分类韩国大米。 构成:用于水稻品种分类的微卫星DNA标记组合由RM48,RM249,RM209,RM70,RM257和OSR20的六(6)个微卫星DNA标记组成,其中它们位于第二,第五,第六,第七,第九和第九 分别在水稻染色体中的第12个位点,并且根据每个PCR扩增的等位基因具有它们自己的两位数。 根据染色体数量,通过6个微卫星标记的组合,每个水稻的各种编号为11位; 并通过PCR扩增和4%丙烯酰胺电泳选择微卫星DNA标记。
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