公开(公告)号：KR100453568B1

公开(公告)日：2004-10-20

申请号：KR1020010034003

申请日：2001-06-15

Applicant: 대한민국(농촌진흥청장)

Inventor： 최해춘 ,  황흥구 ,  김연규 ,  홍하철 ,  권수진 ,  문헌팔 ,  안상낙

IPC: C12Q1/68

Abstract: PURPOSE: Provided are a microsatellite DNA marker combination for classification of rice varieties and specific code numbers given thereby, therefore each of 148 kinds of Korean rice has a specific code number, so that the Korean rice can be easily classified. CONSTITUTION: The microsatellite DNA marker combination for classification of rice varieties consists of six(6) microsatellite DNA markers of RM48, RM249, RM209, RM70, RM257 and OSR20, wherein they are positioned at the 2nd, 5th, 6th, 7th, 9th and 12th sites in a rice chromosome, respectively, and have their own numbers of two digits according to each PCR amplified allele. Each variety of the rice is given code number of 11 digits by combination of the 6 microsatellite markers according to chromosome numbers; and the microsatellite DNA markers are selected by using PCR amplification and 4% acrylamide electrophoresis.

Abstract translation: 目的：提供了用于水稻品种分类的微卫星DNA标记组合和由此给出的具体代码编号，因此148种韩国稻米中的每种具有特定的代码编号，从而韩国稻米可以容易地分类。 构成：用于水稻品种分类的微卫星DNA标记组合由RM48，RM249，RM209，RM70，RM257和OSR20六个（6）微卫星DNA标记组成，分别位于第2,5,6,7,9和 根据每个PCR扩增的等位基因分别具有它们自己的两位数。 根据染色体数目，通过6个微卫星标记的组合给每个品种的11个数字编码; 通过PCR扩增和4％丙烯酰胺电泳筛选微卫星DNA标记。

公开(公告)号：KR1020020095793A

公开(公告)日：2002-12-28

申请号：KR1020010034002

申请日：2001-06-15

Applicant: 대한민국(농촌진흥청장)

Inventor： 최해춘 ,  황흥구 ,  권수진 ,  문헌팔

IPC: C12Q1/68

Abstract: PURPOSE: Provided is a method for coding crops to classify their own variety and to give a coding number to each crop. CONSTITUTION: The method for coding crops to classify varieties thereof comprises the steps of: subjecting DNA markers to PCR amplification, 4% acrylamide gel electrophoresis and silver staining(S100); analyzing each bend pattern for each variety of crops(S200); giving the specific code number of 2 digits to the each variety for each DNA marker according to analysis of the bend pattern(S300); and sequentially giving the specific code numbers to crops according to the order of chromosomes using the numbers given above according to the order of DNA markers present in a chromosome of the plant.

Abstract translation: 目的：提供编码作物以分类自己的品种并给每个作物编码的方法。 构成：将作物分类为品种的方法包括以下步骤：使DNA标记物进行PCR扩增，4％丙烯酰胺凝胶电泳和银染（S100）; 分析每种作物的每种弯曲图案（S200）; 根据弯曲图案的分析给出每个DNA标记的每个品种的2位数的特定代码号（S300）; 并依照染色体的顺序依次按照染色体染色体中存在的DNA标记的顺序，给出上述给出的编号。

公开(公告)号：KR100426467B1

公开(公告)日：2004-04-17

申请号：KR1020010034002

申请日：2001-06-15

Applicant: 대한민국(농촌진흥청장)

Inventor： 최해춘 ,  황흥구 ,  권수진 ,  문헌팔

IPC: C12Q1/68

Abstract: PURPOSE: Provided is a method for coding crops to classify their own variety and to give a coding number to each crop. CONSTITUTION: The method for coding crops to classify varieties thereof comprises the steps of: subjecting DNA markers to PCR amplification, 4% acrylamide gel electrophoresis and silver staining(S100); analyzing each bend pattern for each variety of crops(S200); giving the specific code number of 2 digits to the each variety for each DNA marker according to analysis of the bend pattern(S300); and sequentially giving the specific code numbers to crops according to the order of chromosomes using the numbers given above according to the order of DNA markers present in a chromosome of the plant.

Abstract translation: 目的：提供了一种编码作物的方法，以对它们自己的品种进行分类并给每个作物编号。 构成：编码农作物品种的方法包括以下步骤：对DNA标记进行PCR扩增，4％丙烯酰胺凝胶电泳和银染（S100）; 分析每种作物的每个弯曲模式（S200）; 根据弯曲模式的分析给每个DNA标记的每个品种提供2位的特定代码号（S300）; 并根据存在于植物染色体中的DNA标记的顺序，使用上面给出的数字按照染色体的顺序依次给作物。

公开(公告)号：KR1020020095794A

公开(公告)日：2002-12-28

申请号：KR1020010034003

申请日：2001-06-15

Applicant: 대한민국(농촌진흥청장)

Inventor： 최해춘 ,  황흥구 ,  김연규 ,  홍하철 ,  권수진 ,  문헌팔 ,  안상낙

IPC: C12Q1/68

Abstract: PURPOSE: Provided are a microsatellite DNA marker combination for classification of rice varieties and specific code numbers given thereby, therefore each of 148 kinds of Korean rice has a specific code number, so that the Korean rice can be easily classified. CONSTITUTION: The microsatellite DNA marker combination for classification of rice varieties consists of six(6) microsatellite DNA markers of RM48, RM249, RM209, RM70, RM257 and OSR20, wherein they are positioned at the 2nd, 5th, 6th, 7th, 9th and 12th sites in a rice chromosome, respectively, and have their own numbers of two digits according to each PCR amplified allele. Each variety of the rice is given code number of 11 digits by combination of the 6 microsatellite markers according to chromosome numbers; and the microsatellite DNA markers are selected by using PCR amplification and 4% acrylamide electrophoresis.

Abstract translation: 目的：提供一种用于水稻品种分类的微卫星DNA标记组合和由此给出的具体代码，因此，每种148种韩国大米具有特定的编号，从而可以容易地分类韩国大米。 构成：用于水稻品种分类的微卫星DNA标记组合由RM48，RM249，RM209，RM70，RM257和OSR20的六（6）个微卫星DNA标记组成，其中它们位于第二，第五，第六，第七，第九和第九 分别在水稻染色体中的第12个位点，并且根据每个PCR扩增的等位基因具有它们自己的两位数。 根据染色体数量，通过6个微卫星标记的组合，每个水稻的各种编号为11位; 并通过PCR扩增和4％丙烯酰胺电泳选择微卫星DNA标记。