公开(公告)号：KR1020130066057A

公开(公告)日：2013-06-20

申请号：KR1020110132731

申请日：2011-12-12

Applicant: 대한민국(농촌진흥청장)

Inventor： 백성열 ,  여수환 ,  최혜선 ,  박혜영 ,  윤혜주

IPC: C12N1/20 ,  C12R1/10 ,  A61K35/74

CPC classification number: C12N1/20 ,  A61K35/74 ,  C12R1/10

Abstract: PURPOSE: Bacillus licheniformis YJ11-21(deposit number KACC91684BP) with high productivity of an enzyme with fibrolytic activity is provided to be easily applied to industries, and to save the cost for manufacturing a composition for treating thrombosis. CONSTITUTION: Bacillus licheniformis YJ11-21(deposit number KACC91684BP) produces an enzyme with excellent fibrinolytic activity. The strain is acquired from traditional soy sauce. A method for preparing the strain comprises: a step of smearing the traditional soy sauce on a medium, and culturing; a step of primarily selecting cultured microorganisms according to morphological differences; a step of inoculating the microorganisms on a skim milk plate or a fibrin plate; and a step of selecting a strain which forms a clear zone.

Abstract translation: 目的：提供具有纤维分解活性的酶的高生产率的地衣芽孢杆菌YJ11-21（保藏号KACC91684BP），易于应用于工业，并节省制造用于治疗血栓形成的组合物的成本。 构成：地衣芽孢杆菌YJ11-21（保藏号KACC91684BP）产生具有优异纤维蛋白溶解活性的酶。 该菌株是从传统酱油中获得的。 制备该菌株的方法包括：在培养基上涂抹传统酱油并培养的步骤; 根据形态差异主要选择培养的微生物的步骤; 将微生物接种在脱脂乳板或纤维蛋白板上的步骤; 以及选择形成透明区域的应变的步骤。