Abstract:
본 발명은 새로운 합성 재조합효소 유전자 및 이를 이용한 선발표지 유전자 제거용 식물형질전환 벡터에 관한 것이다. 본 발명에 따른 선발표지 제거용 식물형질전환 벡터 pCMF는 미생물에서 벡터의 안정성이 증가한 반면 식물체에서의 활성은 유지되며, 목표 유전자의 도입과 벡터의 변형이 용이하여 폭넓은 작물 및 다양한 유전자를 이용한 선발표지 제거(marker-free) GM작물 개발에 이용될 수 있을 것이다.
Abstract:
본 발명은 해충 저항성 유전자 변형 농작물의 검정용 프라이머, 프로브 및 이를 이용한 검정 방법에 관한 것으로서, 본 발명에 따른 프라이머 및 프로브는 해충 저항성 벼의 계통을 특이적으로 검출하는 것이 가능하고, 해충저항성 Bt 벼 및 이를 모본으로 하는 파생 품종에 대한 검정이 가능하므로 육종 모본 확인에 활용할 수 있으며, 특히 농산물 및 식품 중에서 해충 저항성 벼를 정성적, 정량적으로 검정함으로써 GMO 농작물 판별에 이용할 수 있다.
Abstract:
PURPOSE: A salt and drought tolerant gene, NtTC gene, and a use thereof are provided to grow a transgenic plant to a variety with salt tolerance and/or drought tolerance. CONSTITUTION: A salt and drought tolerant NtTC protein has an amino acid sequence of sequence number 2. NtTC gene encodes NtTC protein and has a base sequence of sequence number 1. A recombinant expression vector contains NtTc gene. A plant cell with enhanced salt and drought tolerance is transformed with the recombinant expression vector. A method for applying salt and drought tolerance to a plant comprises a step of introducing NtTC gene into a plant.
Abstract:
PURPOSE: A method for detecting a GMO(genetically modified organism) of pest resistance using a primer and a probe is provided to enable specific detection and to determine GMO products. CONSTITUTION: A composition for detecting GMO products contains: 35sBr primer set of sequence numbers 3 and 4; TaCry2 primer set of sequence numbers 5 and 6; CrRB primer set of sequence numbers 7 and 8; and CrLB2 primer set of sequence numbers 9 and 10. The GMO product is a pest resistant Bt rice plant. The Bt rice plant is transformed by a recombinant vector containing CrylAc1 gene. A composition contains a primer for detecting endogenous genes.
Abstract:
PURPOSE: A plant transformation vector, pCMF, for removing a selective marker is provided to enable easy modification of the vector and transduction of a target gene, and to develop marker-free GM(genetically modified) crops. CONSTITUTION: A site-specific recombinase gene, mFLP, has a base in sequence number 1. A vector for transformation in a plant contains the gene, mFLP. The vector contains a stress-induced promoter and recombinase recognition site. The vector is able to remove a selective marker by induction of mFP expression and self-cleavage due to stress. The vector contains MCS(multi-cloning site). A plant is transformed with the vector. Agrobacterium tumefaciens LBA4404 is transformed by the vector and is used as a plant carrier. A transgenic plant is prepared by transforming with the vector and is able to remove the selective marker.