Abstract:
The present invention relates to a novel mannanase gene EM17 derived from intestinal microorganisms which are not capable of being artificially cultured in a larva of Blacksoldier fly (BSF) decomposing food waste effectively; a recombinant vector including the gene; and a transformant transformed by the recombinant vector. The mannanase of EM17 in the present invention is expressed in Escherichia coli and exhibits decomposition ability even in a high organic solvent concentration, a high metal ion concentration and a wide range of acid and base concentrations, thereby reducing an unnecessary purification process in a recycling process of hemicellulose biomass. In addition, the mannanase has various hydrolytic functions and thus can be used for food, feed, coffee extraction, paper and pulp industries, oligosaccharide synthesis, etc.
Abstract:
본 발명은 동애등에 장 내 미생물 유래의 신규 베타-글루코시다제, 상기 베타-글루코시다제를 코딩하는 유전자, 상기 유전자를 포함하는 재조합 벡터, 상기 재조합벡터로 형질전환된 형질전환체에 관한 것이다. 본 발명에 의한 재조합 베타-글루코시다제를 대량 생산하여 농업, 식품, 바이오연료 산업에 실질적으로 이용함으로써 경제성을 제고시킬 수 있다.
Abstract:
The present invention relates to novel beta-glucosidase derived from microorganisms in intestines of Ptecticus tenebrifer, a gene which codes the beta-glucosidase, a recombinant-vector including the genes, and a transformant transformed by the recombinant-vector. Economic feasibility can be improved by mass producing recombined beta-glucosidase of the present invention and actually applying to agricultural, food, and bio-fuel industries.
Abstract:
본 발명은 동애등에 유충 장내에 존재하는 난배양 미생물로부터 분리한 셀룰라아제(cellulase) 유전자 및 이의 용도에 관한 것이다. 본 발명에 따른 셀룰라아제 생산 유전자 CS10은 발현을 이용하여 셀룰로오스를 효과적으로 분해할 수 있고, 저분자 올리고당 물질로 변환시킬 수 있다.
Abstract:
PURPOSE: An antibacterial gene which is specific to phytophthora and a method for preventing plant disease using the same are provided to suppress growth of Phytophthora sp. strain. CONSTITUTION: A gene EnAP95(KACC 95090P) isolated from Enterobacter sp. YNB54 has a base sequence of sequence number 1. The gene EnAP95 is a Sal I fragment which is obtained by: selecting and analyzing E.coli clone which specifically suppresses Phytophthora capsici from a gene bank randomly produced by inserting Enterobacter sp. YNB54 chromosome DNA fragment to pBR322; isolating 20kb of fragment in which anti-phyrophthora gene is inserted; preparing mutant in which bacteriophage Mu is inserted; and selecting mutant of which antagonism to various phytophthora is weakened, lost, or strengthened.