유기용매 하에서 안정성이 높은 신규 만난분해 유전자 EM17과 이의 형질전환 균주로부터 생산된 재조합 만난분해 효소
    4.
    发明公开
    유기용매 하에서 안정성이 높은 신규 만난분해 유전자 EM17과 이의 형질전환 균주로부터 생산된 재조합 만난분해 효소 有权
    有机溶剂耐受性内切-1,4-β-MANNANASE基因EM17及其转化菌株的改造MANNANNASE

    公开(公告)号:KR1020140092079A

    公开(公告)日:2014-07-23

    申请号:KR1020130004378

    申请日:2013-01-15

    Abstract: The present invention relates to a novel mannanase gene EM17 derived from intestinal microorganisms which are not capable of being artificially cultured in a larva of Blacksoldier fly (BSF) decomposing food waste effectively; a recombinant vector including the gene; and a transformant transformed by the recombinant vector. The mannanase of EM17 in the present invention is expressed in Escherichia coli and exhibits decomposition ability even in a high organic solvent concentration, a high metal ion concentration and a wide range of acid and base concentrations, thereby reducing an unnecessary purification process in a recycling process of hemicellulose biomass. In addition, the mannanase has various hydrolytic functions and thus can be used for food, feed, coffee extraction, paper and pulp industries, oligosaccharide synthesis, etc.

    Abstract translation: 本发明涉及一种能够有效地分解食物废弃物的白僵菌幼虫(BSF)幼虫不能人工培养的肠微生物的新型甘露聚糖酶基因EM17。 包含该基因的重组载体; 和由重组载体转化的转化体。 本发明中的EM17的甘露聚糖酶在大肠杆菌中表达,即使在高有机溶剂浓度,高金属离子浓度和广泛的酸碱浓度下也表现出分解能力,从而在再循环过程中减少不必要的纯化过程 的半纤维素生物质。 此外,甘露聚糖酶具有各种水解功能,因此可用于食品,饲料,咖啡提取,造纸和纸浆工业,寡糖合成等。

    동애등에 장 내 미생물 유래의 신규 베타-글루코시다제
    6.
    发明公开
    동애등에 장 내 미생물 유래의 신규 베타-글루코시다제 有权
    BLACKSOLDIER FLY INTESTINAL中微生物衍生的新型β-GLUCOSIDASES

    公开(公告)号:KR1020130136759A

    公开(公告)日:2013-12-13

    申请号:KR1020120060428

    申请日:2012-06-05

    Abstract: The present invention relates to novel beta-glucosidase derived from microorganisms in intestines of Ptecticus tenebrifer, a gene which codes the beta-glucosidase, a recombinant-vector including the genes, and a transformant transformed by the recombinant-vector. Economic feasibility can be improved by mass producing recombined beta-glucosidase of the present invention and actually applying to agricultural, food, and bio-fuel industries.

    Abstract translation: 本发明涉及来源于Ptecticus tenebrifer的肠道中的微生物的新型β-葡糖苷酶,编码β-葡糖苷酶的基因,包含该基因的重组载体和由重组载体转化的转化体。 可以通过大量生产本发明的重组β-葡糖苷酶并且实际应用于农业,食品和生物燃料工业来改进经济可行性。

    파이토프소라속 특이 항균유전자 및 이를 이용한 작물역병 방제 방법
    8.
    发明公开
    파이토프소라속 특이 항균유전자 및 이를 이용한 작물역병 방제 방법 有权
    抗生物多样性基因簇特异性抑制生物多样性SPP的生长。 和其使用的PHYTOPHTHORA的预防方法

    公开(公告)号:KR1020100082143A

    公开(公告)日:2010-07-16

    申请号:KR1020090001489

    申请日:2009-01-08

    Abstract: PURPOSE: An antibacterial gene which is specific to phytophthora and a method for preventing plant disease using the same are provided to suppress growth of Phytophthora sp. strain. CONSTITUTION: A gene EnAP95(KACC 95090P) isolated from Enterobacter sp. YNB54 has a base sequence of sequence number 1. The gene EnAP95 is a Sal I fragment which is obtained by: selecting and analyzing E.coli clone which specifically suppresses Phytophthora capsici from a gene bank randomly produced by inserting Enterobacter sp. YNB54 chromosome DNA fragment to pBR322; isolating 20kb of fragment in which anti-phyrophthora gene is inserted; preparing mutant in which bacteriophage Mu is inserted; and selecting mutant of which antagonism to various phytophthora is weakened, lost, or strengthened.

    Abstract translation: 目的:提供一种特异于植物疫病的抗菌基因和一种防止植物病害的方法,以抑制疫霉疫霉的生长。 应变。 构成:从肠杆菌属分离得到的基因EnAP95(KACC 95090P) YNB54具有序列号1的碱基序列。基因EnAP95是SalI片段,其通过以下方法获得:选择和分析大肠杆菌克隆,其通过插入Enterobacter sp从随机产生的基因库中特异性抑制疫霉疫霉(Phytophthora capsici) YNB54染色体DNA片段至pBR322; 分离其中插入抗植物鸟毒基因的20kb片段; 制备插入噬菌体Mu的突变体; 并选择突变体,其对各种疫霉的拮抗作用被削弱,丧失或增强。

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