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公开(公告)号：KR1020010081456A
公开(公告)日：2001-08-29
申请号：KR1020000006888
申请日：2000-02-14
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/00
CPC classification number: A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/108 , A01K2267/01 , C12N15/8509
Abstract: PURPOSE: A method for producing transgenic porcines for producing human erythropoietin(EPO) is provided, thereby human erythropoietin(EPO) can be mass-produced. CONSTITUTION: The method for producing transgenic porcines comprising the steps of: cloning the genomic DNA of human erythropoietin(EPO) using a WAP promoter isolated from the mammary gland of mouse by polymerase chain reaction(PCR); preparing the EPO expression vector containing human erythropoietin(hEPO) and SV40 poly A gene; injecting eCG hormone and hCG hormone into the fertilized eggs of porcine to induce overovulation; recovering the fertilized eggs and inserting the above DNA into a male cell nucleus using a manipulator; transplanting the cell into a surrogate porcine mother; and producing transgenic porcines having DNA represented by SEQ ID NO: 1 from the surrogate porcine mother.
Abstract translation: 目的：提供生产人促红细胞生成素（EPO）的转基因猪的方法，从而大量生产人促红细胞生成素（EPO）。 构成：生产转基因猪的方法，包括以下步骤：使用通过聚合酶链反应（PCR）从小鼠乳腺分离的WAP启动子克隆人促红细胞生成素（EPO）的基因组DNA; 制备含有促红细胞生成素（hEPO）和SV40聚A基因的EPO表达载体; 将eCG激素和hCG激素注射到猪的受精卵中以诱导过度排卵; 回收受精卵，并使用机械手将上述DNA插入雄性细胞核; 将细胞移植到替代猪母亲中; 并从代孕猪母体生产具有由SEQ ID NO：1表示的DNA的转基因猪。
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公开(公告)号：KR100358754B1
公开(公告)日：2002-11-07
申请号：KR1020000006888
申请日：2000-02-14
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/00
Abstract: 본 발명은 유전공학적인 방법인 유전자 재조합 기술을 이용하여 돼지의 유선에서 인간의 조혈촉진제(EPO:erythropoietin)를 대량으로 생산할 수 있는 형질전환 돼지
(TRANSGENIC PORCINE) 를 생산하는 것에 관한 것으로서, 더 상세하게는 인간의 신장에 존재하는 조혈촉진제(EPO) 유전자를 돼지의 수정란에 주입, 형질전환시키므로서 인간의 조혈촉진제(EPO)유전자를 가지는 돼지(새롬이)를 생산하고 그 돼지의 젖을 통하여 고가의 의약품인 인간의 조혈촉진제를 대량으로 분비할 수 있도록 하므로서 인류건강의 진일보에 기여함은 물론 고가의 의약품 수출을 통하여 국가경제 및 국가경쟁력 기반구축에 크게 기여할 수 있는 특성을 가지는 발명이다.
이에 본 발명의 인간의 조혈촉진제(EPO) 생산을 위한 형질전환 돼지의 생산은 유전자 염기서열이 도 3와 같이 구성되는 것을 특징으로 한다.-
公开(公告)号：KR1020030009936A
公开(公告)日：2003-02-05
申请号：KR1020010044610
申请日：2001-07-24
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/85
CPC classification number: C12N15/8509 , A01K67/0275 , A01K2227/108 , A01K2267/01 , C12N2015/8518
Abstract: PURPOSE: Provided are a transgenic pig capable of producing human erythropoietin and a preparation method thereof, thereby stably and economically producing a large amount of human erythropoietin from the transgenic pig's urine. CONSTITUTION: A recombinant vector of human erythropoietin, pCR2.1 hEPO, includes EPO gene between KpnI of rat U II promotor having EcoRI and KpnI sites and SalI of SV40 Poly A having SalI and EcoRV sites. The transgenic pig is transformed by the recombinant vector, pCR2.1 hEPO and produces human erythropoietin in its urine.
Abstract translation: 目的：提供能够产生人促红细胞生成素的转基因猪及其制备方法，从而稳定且经济地从转基因猪尿中产生大量人促红细胞生成素。 构成：人促红细胞生成素pCR2.1hEPO的重组载体包括具有EcoRI和KpnI位点的大鼠U II启动子的KpnI与具有SalI和EcoRV位点的SV40聚A的SalI之间的EPO基因。 通过重组载体pCR2.1hEPO转化转基因猪，并在其尿液中产生人促红细胞生成素。
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公开(公告)号：KR100453571B1
公开(公告)日：2004-10-20
申请号：KR1020010044610
申请日：2001-07-24
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/85
Abstract: PURPOSE: Provided are a transgenic pig capable of producing human erythropoietin and a preparation method thereof, thereby stably and economically producing a large amount of human erythropoietin from the transgenic pig's urine. CONSTITUTION: A recombinant vector of human erythropoietin, pCR2.1 hEPO, includes EPO gene between KpnI of rat U II promotor having EcoRI and KpnI sites and SalI of SV40 Poly A having SalI and EcoRV sites. The transgenic pig is transformed by the recombinant vector, pCR2.1 hEPO and produces human erythropoietin in its urine.
Abstract translation: 发明目的：提供一种能够产生人促红细胞生成素的转基因猪及其制备方法，由此从转基因猪尿中稳定而经济地生产大量人促红细胞生成素。 构成：人促红细胞生成素的重组载体pCR2.1hEPO包含具有EcoRI和KpnI位点的大鼠UII启动子的KpnI与具有SalI和EcoRV位点的SV40多聚A的SalI之间的EPO基因。 通过重组载体pCR2.1hEPO转化转基因猪并在其尿中产生人促红细胞生成素。
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