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公开(公告)号：KR100842432B1
公开(公告)日：2008-07-01
申请号：KR1020070035741
申请日：2007-04-11
Applicant: 대한민국(농촌진흥청장)
Abstract: An SSR(simple sequence repeat) primer pair is provided to be usefully used for DNA profiling of mandarin by detecting DNA polymorphism of the mandarin, thereby evaluating genetic source of the mandarin efficiently. An SSR primer pair is selected from the group consisting of: a primer pair of SEQ ID : NOs. 1 and 2; a primer pair of SEQ ID : NOs. 3 and 4; a primer pair of SEQ ID : NOs. 5 and 6; a primer pair of SEQ ID : NOs. 7 and 8; a primer pair of SEQ ID : NOs. 9 and 10; a primer pair of SEQ ID : NOs. 11 and 12; a primer pair of SEQ ID : NOs. 13 and 14; a primer pair of SEQ ID : NOs. 15 and 16; a primer pair of SEQ ID : NOs. 17 and 18; a primer pair of SEQ ID : NOs. 19 and 20; a primer pair of SEQ ID : NOs. 21 and 22; a primer pair of SEQ ID : NOs. 23 and 24; a primer pair of SEQ ID : NOs. 25 and 26; a primer pair of SEQ ID : NOs. 27 and 28; a primer pair of SEQ ID : NOs. 29 and 30; a primer pair of SEQ ID : NOs. 31 and 32; a primer pair of SEQ ID : NOs. 33 and 34; a primer pair of SEQ ID : NOs. 35 and 36; a primer pair of SEQ ID : NOs. 37 and 38; a primer pair of SEQ ID : NOs. 39 and 40; a primer pair of SEQ ID : NOs. 41 and 42; a primer pair of SEQ ID : NOs. 43 and 44; a primer pair of SEQ ID : NOs. 45 and 46; a primer pair of SEQ ID : NOs. 47 and 48; a primer pair of SEQ ID : NOs. 49 and 50; a primer pair of SEQ ID : NOs. 51 and 52; a primer pair of SEQ ID : NOs. 53 and 54; a primer pair of SEQ ID : NOs. 55 and 56; a primer pair of SEQ ID : NOs. 57 and 58; a primer pair of SEQ ID : NOs. 59 and 60 and is derived from mandarin(Citrus unshiu Markovich). A kit for detecting the DNA polymorphism of the mandarin or identifying species of the mandarin comprises the SSR primer pair.
Abstract translation: 提供SSR（简单序列重复）引物对，通过检测普通话的DNA多态性有效地用于普通话的DNA分析，从而有效地评估汉语的遗传来源。 SSR引物对选自SEQ ID NO：的引物对。 1和2; SEQ ID NO：1的引物对。 3和4; SEQ ID NO：1的引物对。 5和6; SEQ ID NO：1的引物对。 7和8; SEQ ID NO：1的引物对。 9和10; SEQ ID NO：1的引物对。 11和12; SEQ ID NO：1的引物对。 13和14; SEQ ID NO：1的引物对。 15和16; SEQ ID NO：1的引物对。 17和18; SEQ ID NO：1的引物对。 19和20; SEQ ID NO：1的引物对。 21和22; SEQ ID NO：1的引物对。 23和24; SEQ ID NO：1的引物对。 25和26; SEQ ID NO：1的引物对。 27和28; SEQ ID NO：1的引物对。 29和30; SEQ ID NO：1的引物对。 31和32; SEQ ID NO：1的引物对。 33和34; SEQ ID NO：1的引物对。 35和36; SEQ ID NO：1的引物对。 37和38; SEQ ID NO：1的引物对。 39和40; SEQ ID NO：1的引物对。 41和42; SEQ ID NO：1的引物对。 43和44; SEQ ID NO：1的引物对。 45和46; SEQ ID NO：1的引物对。 47和48; SEQ ID NO：1的引物对。 49和50; SEQ ID NO：1的引物对。 51和52; SEQ ID NO：1的引物对。 53和54; SEQ ID NO：1的引物对。 55和56; SEQ ID NO：1的引物对。 57和58; SEQ ID NO：1的引物对。 59和60，来源于柑橘（Citrus unshiu Markovich）。 用于检测普通话的DNA多态性或鉴定柑橘的物种的试剂盒包括SSR引物对。
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Patent Agency Ranking
公开(公告)号：KR100842430B1
公开(公告)日：2008-07-01
申请号：KR1020070035740
申请日：2007-04-11
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q2600/156
Abstract: An SSR(simple sequence repeat) primer pair is provided to detect DNA polymorphism from Japanese Apricot and being usefully used to analyze genetic diversity, thereby efficiently evaluating and preserving genetic sources of the Japanese Apricot. An SSR primer pair comprises two sequences selected from the group consisting of SEQ ID : NOs. 1 to 60 and is derived from Japanese Apricot(Prunus mume S. et Z.). A method for detecting DNA polymorphism of the Japanese Apricot comprises the steps of: (a) extracting genome DNA from the lawn grass; (b) subjecting the extracted genome DNA as a template to PCR using the SSR primer pair; and (c) isolating PCR products obtained from the step(b) by size. A method for identifying species of the Japanese Apricot comprises the steps of: (a) extracting genome DNA from the Japanese Apricot and a control group Japanese Apricot species, respectively; (b) subjecting each of the extracted genome DNA to PCR using the SSR primer pair; (c) isolating each of PCR products obtained from the step(b) by size; and (d) comparing isolation results by the size of the Japanese Apricot with that of the control group.
Abstract translation: 提供SSR（简单序列重复）引物对以检测日本杏的DNA多态性，并有效地用于分析遗传多样性，从而有效评估和保存日本杏的遗传来源。 SSR引物对包含选自SEQ ID NO： 1至60，并衍生自日本杏（Prunus mume S.et Z.）。 检测日本杏的DNA多态性的方法包括以下步骤：（a）从草坪提取基因组DNA; （b）使用SSR引物对将提取的基因组DNA作为模板进行PCR; 和（c）通过尺寸分离由步骤（b）获得的PCR产物。 用于鉴定日本杏种的方法包括以下步骤：（a）分别从日本杏和对照组日本杏提取基因组DNA; （b）使用SSR引物对对所提取的基因组DNA进行PCR; （c）通过大小分离从步骤（b）获得的每个PCR产物; 和（d）将隔离结果与日本杏的大小与对照组的大小进行比较。
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公开(公告)号：KR100842446B1
公开(公告)日：2008-07-01
申请号：KR1020070035744
申请日：2007-04-11
Applicant: 대한민국(농촌진흥청장)
Abstract: An SSR(simple sequence repeat) primer pair derived from ginger is provided to detect DNA polymorphism of the ginger effectively and be very usefully used for DNA profiling of the ginger, thereby analyzing whether a newly introduced genetic resource is duplicated with a conventional resource and securing novelty of the newly introduced genetic resource by evaluating the genetic source of the ginger efficiently. An SSR primer pair is selected from the group consisting of: a primer pair of SEQ ID : NOs. 1 and 2; a primer pair of SEQ ID : NOs. 3 and 4; a primer pair of SEQ ID : NOs. 5 and 6; a primer pair of SEQ ID : NOs. 7 and 8; a primer pair of SEQ ID : NOs. 9 and 10; a primer pair of SEQ ID : NOs. 11 and 12; a primer pair of SEQ ID : NOs. 13 and 14; and a primer pair of SEQ ID : NOs. 15 and 16 and is derived from ginger(Zingiber officinale Rosc.). A method for detecting DNA polymorphism of the ginger comprises the steps of: (a) extracting genome DNA from the ginger; (b) subjecting the extracted genome DNA as a template to PCR using the SSR primer pair; and (c) isolating PCR products obtained from the step(b) by size. A method for identifying species of the ginger comprises the steps of: (a) extracting genome DNA from the ginger and a control group ginger species, respectively; (b) subjecting each of the extracted genome DNA to PCR using the SSR primer pair; (c) isolating each of PCR products obtained from the step(b) by size; and (d) comparing isolation results by the size of the ginger with that of the control group. A kit for detecting the DNA polymorphism of the ginger or identifying species of the ginger comprises the SSR primer pair.
Abstract translation: 提供从姜中获得的SSR（简单序列重复）引物对，有效检测生姜的DNA多态性，非常有用地用于生姜的DNA分析，从而分析新引入的遗传资源是否与传统资源重复 通过有效地评估姜的遗传来源，新引入的遗传资源的新颖性。 SSR引物对选自SEQ ID NO：的引物对。 1和2; SEQ ID NO：1的引物对。 3和4; SEQ ID NO：1的引物对。 5和6; SEQ ID NO：1的引物对。 7和8; SEQ ID NO：1的引物对。 9和10; SEQ ID NO：1的引物对。 11和12; SEQ ID NO：1的引物对。 13和14; 和SEQ ID NO：的引物对。 15和16，来自姜（Zingiber officinale Rosc。）。 用于检测生姜的DNA多态性的方法包括以下步骤：（a）从姜提取基因组DNA; （b）使用SSR引物对将提取的基因组DNA作为模板进行PCR; 和（c）通过尺寸分离由步骤（b）获得的PCR产物。 用于鉴定姜的种类的方法包括以下步骤：（a）从姜和对照组姜分别提取基因组DNA; （b）使用SSR引物对对所提取的基因组DNA进行PCR; （c）通过大小分离从步骤（b）获得的每个PCR产物; 和（d）将分离结果与姜的大小与对照组的大小进行比较。 用于检测生姜的DNA多态性或识别姜的种类的试剂盒包含SSR引物对。
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公开(公告)号：KR100803392B1
公开(公告)日：2008-02-14
申请号：KR1020060076809
申请日：2006-08-14
Applicant: 대한민국(농촌진흥청장)
Abstract: An SSR primer pair is provided to be able to be very usefully used for preparing a DNA profile of Coix lachryma-jobi by effectively detecting a DNA polymorphism and effectively analyze the redundancy with conventional resources and establish the novelty when a novel genetic source is introduced by efficiently evaluating the genetic source of the Coix lachryma-jobi. An SSR primer pair derived from Coix lachryma-jobi is selected from the group consisting of a primer pair described as SEQ ID : NOs. 1 and 2; a primer pair described as SEQ ID : NOs. 3 and 4; a primer pair described as SEQ ID : NOs. 5 and 6; a primer pair described as SEQ ID : NOs. 7 and 8; a primer pair described as SEQ ID : NOs. 9 and 10; a primer pair described as SEQ ID : NOs. 11 and 12; a primer pair described as SEQ ID : NOs. 13 and 14; a primer pair described as SEQ ID : NOs. 15 and 16; a primer pair described as SEQ ID : NOs. 17 and 18; a primer pair described as SEQ ID : NOs. 19 and 20; a primer pair described as SEQ ID : NOs. 21 and 22; a primer pair described as SEQ ID : NOs. 23 and 24; a primer pair described as SEQ ID : NOs. 25 and 26; a primer pair described as SEQ ID : NOs. 27 and 28; a primer pair described as SEQ ID : NOs. 29 and 30; a primer pair described as SEQ ID : NOs. 31 and 32; and a primer pair described as SEQ ID : NOs. 33 and 34. A method for detecting a DNA polymorphism of Coix lachryma-jobi comprises the steps of: (a) extracting a genome DNA from the Coix lachryma-jobi; (b) performing a PCR using the extracted genome DNA as a template and the SSR primer pair; and (c) isolating PCR products by size.
Abstract translation: 提供了一种SSR引物对，可以非常有效地用于通过有效检测DNA多态性并有效地分析冗余与传统资源，并建立新颖的遗传源由新的遗传源引入时，通过 有效评估枸杞子的遗传来源。 衍生自枸杞子的SSR引物对选自如SEQ ID NO：所示的引物对。 1和2; 如SEQ ID NO：所示的引物对。 3和4; 如SEQ ID NO：所示的引物对。 5和6; 如SEQ ID NO：所示的引物对。 7和8; 如SEQ ID NO：所示的引物对。 9和10; 如SEQ ID NO：所示的引物对。 11和12; 如SEQ ID NO：所示的引物对。 13和14; 如SEQ ID NO：所示的引物对。 15和16; 如SEQ ID NO：所示的引物对。 17和18; 如SEQ ID NO：所示的引物对。 19和20; 如SEQ ID NO：所示的引物对。 21和22; 如SEQ ID NO：所示的引物对。 23和24; 如SEQ ID NO：所示的引物对。 25和26; 如SEQ ID NO：所示的引物对。 27和28; 如SEQ ID NO：所示的引物对。 29和30; 如SEQ ID NO：所示的引物对。 31和32; 和如SEQ ID NO：所示的引物对。 一种检测枸杞子DNA多态性的方法，包括以下步骤：（a）从枸杞子提取基因组DNA; （b）使用提取的基因组DNA作为模板和SSR引物对进行PCR; 和（c）按大小分离PCR产物。
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公开(公告)号：KR100769366B1
公开(公告)日：2007-10-31
申请号：KR1020060094921
申请日：2006-09-28
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6827 , C12Q1/686
Abstract: An SSR(simple sequence repeat) primer pair is provided to be very usefully used for preparing a DNA profile of Vigna radiata L. by effectively detecting the DNA polymorphism of the Vigna radiata L., thereby capable of performing redundancy analysis with conventional resources when introducing a novel genetic resource and determining species of the Vigna radiata L.. An SSR primer pair has two sequences selected from the group consisting of SEQ ID : NOs. 1-14 and is derived from Vigna radiata L.. A method for detecting DNA polymorphism of Vigna radiata L. comprises the steps of: (a) extracting genome DNA from Vigna radiata L.; (b) performing PCR on the extracted genome DNA as a template using the SSR primer pair; and (c) isolating the PCR product by size. A method for identifying species of Vigna radiata L. comprises the steps of: (a) extracting genome DNAs from Vigna radiata L. and a control Vigna radiata L. species; (b) performing PCR on each of the extracted genome DNAs using the SSR primer pair; (c) isolating each of the PCR products by size; and (d) comparing the isolation results of the Vigna radiata L. and the control Vigna radiata L. species.
Abstract translation: 提供SSR（简单序列重复）引物对非常有用地用于通过有效地检测Vigna radiata L.的DNA多态性来制备Vigna radiata L.的DNA谱，从而能够在引入时用常规资源进行冗余分析 一种新型遗传资源和确定Vigna radiata L的物种.SSSR引物对具有选自SEQ ID NO： 1-14，衍生自Vigna radiata L。一种用于检测鞭毛虫DNA的多态性的方法，包括以下步骤：（a）从梵氏菌提取基因组DNA。 （b）使用SSR引物对在提取的基因组DNA上作为模板进行PCR; 和（c）按大小分离PCR产物。 一种用于鉴定芸香属种的方法，包括以下步骤：（a）从梵氏菌（Vigna radiata L.）提取基因组DNA，以及控制芸苔（Vigna radiata L.）种; （b）使用SSR引物对对每个提取的基因组DNA进行PCR; （c）按大小分离每个PCR产物; 和（d）比较Vigna radiata L.和对照Vigna radiata L.物种的分离结果。
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公开(公告)号：KR100769367B1
公开(公告)日：2007-10-31
申请号：KR1020060094920
申请日：2006-09-28
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6827 , C12Q1/686
Abstract: An SSR(simple sequence repeat) primer from Panicum miliaceum L. is provided to be very usefully used for preparing a DNA profile of the Panicum miliaceum L. by effectively detecting the DNA polymorphism of the Panicum miliaceum L. and efficiently evaluate genetic resources of the Panicum miliaceum L., thereby effectively performing redundancy analysis with conventional resources and establishing novelty when a novel genetic resource is introduced and being able to determine the species of the Panicum miliaceum L.. An SSR primer pair derived from Panicum miliaceum L. has two sequences selected from the group consisting of SEQ ID : NOs. 1-62. A method for identifying species of Panicum miliaceum L. comprises the steps of: (a) extracting genome DNAs from Panicum miliaceum L. and a control Panicum miliaceum L. species; (b) performing PCR on each of the extracted genome DNAs using the SSR primer pair; (c) isolating each of the PCR products by size; and (d) comparing the isolation results of the Panicum miliaceum L. and the control Panicum miliaceum L. species. A kit for detecting DNA polymorphism or a kit for identifying species of Panicum miliaceum L. comprises the SSR primer pair.
Abstract translation: 提供了一种来自黍属的SSR（简单序列重复）引物，非常有用地用于通过有效检测黍属的DNA多态性来有效地评估黍属的DNA多态性，有效地评估了黍属的遗传资源 从而有效地进行了常规资源的冗余分析，并且当引入新的遗传资源并且能够确定黍属的种类时，建立新颖性。来自黍属的SSR引物对具有两个序列 选自SEQ ID NO： 1-62。 一种用于鉴定黍属种的方法，包括以下步骤：（a）从黍黍提取基因组DNA和对照黍螨种; （b）使用SSR引物对对每个提取的基因组DNA进行PCR; （c）按大小分离每个PCR产物; 和（d）比较黍螨和对照黍属的分离结果。 用于检测DNA多态性的试剂盒或用于鉴定黍属的种类的试剂盒包含SSR引物对。
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