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公开(公告)号：KR1020100110524A
公开(公告)日：2010-10-13
申请号：KR1020090028897
申请日：2009-04-03
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A vitrification solution and loading solution are provided to enhance high usage and to improve viability and regeneration rate. CONSTITUTION: A vitrification solution contains 42.5 of glycerol, 15 of dimethylsulfoxide, 15 of ethylene glycol, and 17.5 of sucrose; 37.5 of glycerol, 15 of dimethylsulfoxide, 15 of ethylene glycol, and 22.5 of sucrose; 32.5 of glycerol, 15 of dimethylsulfoxide, 15 of ethylene glycol, and 27.5 of sucrose; and 35.0 of glycerol, 15 of dimethylsolfoxide, 15 of ethylene glycol, and 20.0 of sucrose (%, w/v). A loading solution contains 9.2 of 1.0 M glycerol, 23.9 of 0.70M of sucrose, 18.4 of 2.0 M glycerol and 22.25 of 0.65M sucrose; and 15.0 of 1.63M glycerol, 15.0 of 0.44M sucrose, 17.5 of 1.9M glycerol, and 17.5 of 0.51M of sucrose.
Abstract translation: 目的：提供玻璃化溶液和加载溶液，以提高高使用率和改善活力和再生率。 构成：玻璃化溶液含有42.5份甘油，15份二甲基亚砜，15份乙二醇和17.5份蔗糖; 37.5份甘油，15份二甲基亚砜，15份乙二醇和22.5份蔗糖; 32.5的甘油，15的二甲基亚砜，15的乙二醇和27.5的蔗糖; 和35.0的甘油，15的二甲基氧化物，15的乙二醇和20.0的蔗糖（％，w / v）。 加载溶液含有9.2的1.0M甘油，23.9的0.7M的蔗糖，18.4的2.0M甘油和22.25的0.65M蔗糖; 和15.0的1.63M甘油，15.0的0.44M蔗糖，17.5的1.9M甘油和17.5的0.51M的蔗糖。
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公开(公告)号：KR1020100088936A
公开(公告)日：2010-08-11
申请号：KR1020090008085
申请日：2009-02-02
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A self-brewer and a method for manufacturing spirituous liquor using the same are provided to make and enjoy spirituous liquor at home. CONSTITUTION: A self-brewer comprises: an electric rice cooker(10) having heat keeping function and cooking function; a distillation device(20) which distils fermentation liquid and is connected to the electric rice cooker; a cooling device(40) for cooling a distillation pipe; and the distillation pipe(30) which is connected to the electric rice cooker.
Abstract translation: 目的：提供自酿啤酒和使用该啤酒制造烈酒的方法，以在家中制作和享用烈酒。 构成：自酿啤酒机包括：具有保温功能和烹饪功能的电饭煲（10） 蒸馏装置（20），其蒸发发酵液并连接到电饭煲; 用于冷却蒸馏管的冷却装置（40）; 和与电饭煲连接的蒸馏管（30）。
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公开(公告)号：KR100769367B1
公开(公告)日：2007-10-31
申请号：KR1020060094920
申请日：2006-09-28
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6827 , C12Q1/686
Abstract: An SSR(simple sequence repeat) primer from Panicum miliaceum L. is provided to be very usefully used for preparing a DNA profile of the Panicum miliaceum L. by effectively detecting the DNA polymorphism of the Panicum miliaceum L. and efficiently evaluate genetic resources of the Panicum miliaceum L., thereby effectively performing redundancy analysis with conventional resources and establishing novelty when a novel genetic resource is introduced and being able to determine the species of the Panicum miliaceum L.. An SSR primer pair derived from Panicum miliaceum L. has two sequences selected from the group consisting of SEQ ID : NOs. 1-62. A method for identifying species of Panicum miliaceum L. comprises the steps of: (a) extracting genome DNAs from Panicum miliaceum L. and a control Panicum miliaceum L. species; (b) performing PCR on each of the extracted genome DNAs using the SSR primer pair; (c) isolating each of the PCR products by size; and (d) comparing the isolation results of the Panicum miliaceum L. and the control Panicum miliaceum L. species. A kit for detecting DNA polymorphism or a kit for identifying species of Panicum miliaceum L. comprises the SSR primer pair.
Abstract translation: 提供了一种来自黍属的SSR（简单序列重复）引物，非常有用地用于通过有效检测黍属的DNA多态性来有效地评估黍属的DNA多态性，有效地评估了黍属的遗传资源 从而有效地进行了常规资源的冗余分析，并且当引入新的遗传资源并且能够确定黍属的种类时，建立新颖性。来自黍属的SSR引物对具有两个序列 选自SEQ ID NO： 1-62。 一种用于鉴定黍属种的方法，包括以下步骤：（a）从黍黍提取基因组DNA和对照黍螨种; （b）使用SSR引物对对每个提取的基因组DNA进行PCR; （c）按大小分离每个PCR产物; 和（d）比较黍螨和对照黍属的分离结果。 用于检测DNA多态性的试剂盒或用于鉴定黍属的种类的试剂盒包含SSR引物对。
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公开(公告)号：KR101031348B1
公开(公告)日：2011-04-27
申请号：KR1020090008085
申请日：2009-02-02
Applicant: 대한민국(농촌진흥청장)
Abstract: 보온기능과 취사기능을 구비하여 취사가 가능하도록 마련된 전기밥솥과, 이 전기밥솥에 착탈 가능하도록 결합되는 증류장치를 포함하도록 구성되는 자가양조기와, 이러한 자가양조기를 이용하여 증류장치가 분리된 전기밥솥을 통해 양조원료를 발효시키고 전기밥솥에 증류장치를 연결하여 전기밥솥 내부의 양조원료 발효액을 증류시켜 증류주를 제조하는 증류주 제조방법이 개시된다.
따라서 본 발명에 따른 자가양조기 및 이를 이용한 증류주 제조방법을 이용하게 되면, 일반 가정에서 누구나 쉽게 전통주와 같은 증류주를 제조하여 음용할 수 있게 되며, 본 발명에 따른 자가양조기는 전기밥솥을 증류장치가 분리된 상태에서 일반 전기밥솥과 다름없이 취사용으로 사용할 수 있게 되어 경제적이다.-
公开(公告)号：KR100842429B1
公开(公告)日：2008-07-01
申请号：KR1020070035739
申请日：2007-04-11
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q2600/156
Abstract: An SSR(simple sequence repeat) primer pair is provided to be very useful for DNA profiling of lawn grass by detecting DNA polymorphism of the lawn grass effectively, thereby being used to analyze whether a newly introduced genetic resource is duplicated with a conventional resource and securing novelty of the newly introduced genetic resource and identifying species of the lawn grass. An SSR primer pair comprises two sequences selected from the group consisting of SEQ ID : NOs. 1 to 60 and is derived from lawn grass(Zoysia japonica steud). A method for detecting DNA polymorphism of the lawn grass comprises the steps of: (a) extracting genome DNA from the lawn grass; (b) subjecting the extracted genome DNA as a template to PCR using the SSR primer pair; and (c) isolating PCR products obtained from the step(b) by size. A kit for detecting the DNA polymorphism of the lawn grass or identifying species of the lawn grass comprises the SSR primer pair.
Abstract translation: 提供SSR（简单序列重复）引物对通过有效检测草坪草的DNA多态性对草坪草的DNA分析非常有用，从而用于分析新引入的遗传资源是否与常规资源复制并确保 新引入的遗传资源和识别草坪草种类的新颖性。 SSR引物对包含选自SEQ ID NO： 1〜60，来自草坪草（Zoysia japonica steud）。 一种检测草坪草DNA多态性的方法，包括以下步骤：（a）从草坪提取基因组DNA; （b）使用SSR引物对将提取的基因组DNA作为模板进行PCR; 和（c）通过尺寸分离由步骤（b）获得的PCR产物。 用于检测草坪草的DNA多态性或鉴定草坪草的种类的试剂盒包括SSR引物对。
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Patent Agency Ranking
公开(公告)号：KR1020110008913A
公开(公告)日：2011-01-27
申请号：KR1020090066472
申请日：2009-07-21
Applicant: 대한민국(농촌진흥청장) , 대윤계기산업 주식회사
CPC classification number: G01N33/02
Abstract: PURPOSE: A sensor for measuring salty taste is provided to simply determine salty taste in case that the salty taste is less than or more than preset value. CONSTITUTION: A sensor for measuring salty taste of pocket type comprises: a hollow sensor body(10) with a circuit substrate(30) and battery; a cell block(50) wherein one end is inserted into an end of the sensor body and other end has a groove and protrusion with a salty taste sensor hole; a salty taste measurement sensor which is which inserted to the salty taste sensor hole and is connected to the circuit substrate; a temperature sensor which is connected to the circuit substrate; an on/off switch(80) which is mounted at one end of opposite cell block and is connected to the circuit substrate and battery; a light emitting lamp which is connected to the circuit substrate; and a sensor cover(100) having an operation piece(103) for operating the on/off switch.
Abstract translation: 目的：提供用于测量咸味的传感器，以便在咸味小于或超过预设值的情况下简单地确定咸味。 构成：用于测量袋型咸味的传感器包括：具有电路基板（30）和电池的中空传感器体（10）; 细胞块（50），其一端插入到传感器主体的一端，另一端具有带有咸味传感器孔的凹槽和凸起; 咸味测量传感器，其被插入到咸味传感器孔中并连接到电路基板; 连接到电路基板的温度传感器; 一个开/关开关（80），其安装在相对的单元块的一端并连接到电路基板和电池; 连接到电路基板的发光灯; 以及具有用于操作所述开/关开关的操作件（103）的传感器盖（100）。
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公开(公告)号：KR100842432B1
公开(公告)日：2008-07-01
申请号：KR1020070035741
申请日：2007-04-11
Applicant: 대한민국(농촌진흥청장)
Abstract: An SSR(simple sequence repeat) primer pair is provided to be usefully used for DNA profiling of mandarin by detecting DNA polymorphism of the mandarin, thereby evaluating genetic source of the mandarin efficiently. An SSR primer pair is selected from the group consisting of: a primer pair of SEQ ID : NOs. 1 and 2; a primer pair of SEQ ID : NOs. 3 and 4; a primer pair of SEQ ID : NOs. 5 and 6; a primer pair of SEQ ID : NOs. 7 and 8; a primer pair of SEQ ID : NOs. 9 and 10; a primer pair of SEQ ID : NOs. 11 and 12; a primer pair of SEQ ID : NOs. 13 and 14; a primer pair of SEQ ID : NOs. 15 and 16; a primer pair of SEQ ID : NOs. 17 and 18; a primer pair of SEQ ID : NOs. 19 and 20; a primer pair of SEQ ID : NOs. 21 and 22; a primer pair of SEQ ID : NOs. 23 and 24; a primer pair of SEQ ID : NOs. 25 and 26; a primer pair of SEQ ID : NOs. 27 and 28; a primer pair of SEQ ID : NOs. 29 and 30; a primer pair of SEQ ID : NOs. 31 and 32; a primer pair of SEQ ID : NOs. 33 and 34; a primer pair of SEQ ID : NOs. 35 and 36; a primer pair of SEQ ID : NOs. 37 and 38; a primer pair of SEQ ID : NOs. 39 and 40; a primer pair of SEQ ID : NOs. 41 and 42; a primer pair of SEQ ID : NOs. 43 and 44; a primer pair of SEQ ID : NOs. 45 and 46; a primer pair of SEQ ID : NOs. 47 and 48; a primer pair of SEQ ID : NOs. 49 and 50; a primer pair of SEQ ID : NOs. 51 and 52; a primer pair of SEQ ID : NOs. 53 and 54; a primer pair of SEQ ID : NOs. 55 and 56; a primer pair of SEQ ID : NOs. 57 and 58; a primer pair of SEQ ID : NOs. 59 and 60 and is derived from mandarin(Citrus unshiu Markovich). A kit for detecting the DNA polymorphism of the mandarin or identifying species of the mandarin comprises the SSR primer pair.
Abstract translation: 提供SSR（简单序列重复）引物对，通过检测普通话的DNA多态性有效地用于普通话的DNA分析，从而有效地评估汉语的遗传来源。 SSR引物对选自SEQ ID NO：的引物对。 1和2; SEQ ID NO：1的引物对。 3和4; SEQ ID NO：1的引物对。 5和6; SEQ ID NO：1的引物对。 7和8; SEQ ID NO：1的引物对。 9和10; SEQ ID NO：1的引物对。 11和12; SEQ ID NO：1的引物对。 13和14; SEQ ID NO：1的引物对。 15和16; SEQ ID NO：1的引物对。 17和18; SEQ ID NO：1的引物对。 19和20; SEQ ID NO：1的引物对。 21和22; SEQ ID NO：1的引物对。 23和24; SEQ ID NO：1的引物对。 25和26; SEQ ID NO：1的引物对。 27和28; SEQ ID NO：1的引物对。 29和30; SEQ ID NO：1的引物对。 31和32; SEQ ID NO：1的引物对。 33和34; SEQ ID NO：1的引物对。 35和36; SEQ ID NO：1的引物对。 37和38; SEQ ID NO：1的引物对。 39和40; SEQ ID NO：1的引物对。 41和42; SEQ ID NO：1的引物对。 43和44; SEQ ID NO：1的引物对。 45和46; SEQ ID NO：1的引物对。 47和48; SEQ ID NO：1的引物对。 49和50; SEQ ID NO：1的引物对。 51和52; SEQ ID NO：1的引物对。 53和54; SEQ ID NO：1的引物对。 55和56; SEQ ID NO：1的引物对。 57和58; SEQ ID NO：1的引物对。 59和60，来源于柑橘（Citrus unshiu Markovich）。 用于检测普通话的DNA多态性或鉴定柑橘的物种的试剂盒包括SSR引物对。
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公开(公告)号：KR100842430B1
公开(公告)日：2008-07-01
申请号：KR1020070035740
申请日：2007-04-11
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q2600/156
Abstract: An SSR(simple sequence repeat) primer pair is provided to detect DNA polymorphism from Japanese Apricot and being usefully used to analyze genetic diversity, thereby efficiently evaluating and preserving genetic sources of the Japanese Apricot. An SSR primer pair comprises two sequences selected from the group consisting of SEQ ID : NOs. 1 to 60 and is derived from Japanese Apricot(Prunus mume S. et Z.). A method for detecting DNA polymorphism of the Japanese Apricot comprises the steps of: (a) extracting genome DNA from the lawn grass; (b) subjecting the extracted genome DNA as a template to PCR using the SSR primer pair; and (c) isolating PCR products obtained from the step(b) by size. A method for identifying species of the Japanese Apricot comprises the steps of: (a) extracting genome DNA from the Japanese Apricot and a control group Japanese Apricot species, respectively; (b) subjecting each of the extracted genome DNA to PCR using the SSR primer pair; (c) isolating each of PCR products obtained from the step(b) by size; and (d) comparing isolation results by the size of the Japanese Apricot with that of the control group.
Abstract translation: 提供SSR（简单序列重复）引物对以检测日本杏的DNA多态性，并有效地用于分析遗传多样性，从而有效评估和保存日本杏的遗传来源。 SSR引物对包含选自SEQ ID NO： 1至60，并衍生自日本杏（Prunus mume S.et Z.）。 检测日本杏的DNA多态性的方法包括以下步骤：（a）从草坪提取基因组DNA; （b）使用SSR引物对将提取的基因组DNA作为模板进行PCR; 和（c）通过尺寸分离由步骤（b）获得的PCR产物。 用于鉴定日本杏种的方法包括以下步骤：（a）分别从日本杏和对照组日本杏提取基因组DNA; （b）使用SSR引物对对所提取的基因组DNA进行PCR; （c）通过大小分离从步骤（b）获得的每个PCR产物; 和（d）将隔离结果与日本杏的大小与对照组的大小进行比较。
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公开(公告)号：KR100918018B1
公开(公告)日：2009-09-24
申请号：KR1020070035743
申请日：2007-04-11
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 아마란스에서 분리한 SSR 프라이머 및 이의 용도에 관한 것으로서, 보다 상세하게는 서열번호 1 내지 서열번호 24로 이루어진 군에서 선택되는 2개의 염기서열을 갖는 SSR 프라이머쌍 및 이를 이용하여 PCR을 수행하는 것을 포함하는 아마란스의 DNA 다형성 검출 방법에 관한 것이다. 본 발명에서 제공되는 SSR 프라이머쌍은 아마란스의 DNA 다형성을 효과적으로 검출할 수 있으며, 아마란스의 DNA 프로파일을 작성하는데 매우 유용하게 이용될 수 있다. 또한, 이를 통해 아마란스의 유전자원을 효율적으로 평가함으로써 신규 유전자원 도입시 기존 보유자원과의 중복성 분석 및 신규성 확립을 효과적으로 수행할 수 있다.
SSR 프라이머, 아마란스, DNA 다형성
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