公开(公告)号：KR101077208B1

公开(公告)日：2011-10-31

申请号：KR1020080049023

申请日：2008-05-27

Applicant: 대한민국(농촌진흥청장)

Inventor： 이수영 ,  한봉희 ,  우종규 ,  방창석 ,  허건양 ,  최영임

IPC: C12N5/10 ,  C12N5/04 ,  C12N15/09 ,  C12N15/29

Abstract: 본발명에의하면건조, 저온, 습도및 중금속또는대기오염등과같은비 생물적스트레스에저항성을갖는페튜니아(종자)를개발할수 있다.

公开(公告)号：KR1020090123108A

公开(公告)日：2009-12-02

申请号：KR1020080049023

申请日：2008-05-27

Applicant: 대한민국(농촌진흥청장)

Inventor： 이수영 ,  한봉희 ,  우종규 ,  방창석 ,  허건양 ,  최영임

IPC: C12N5/10 ,  C12N5/04 ,  C12N15/09 ,  C12N15/29

Abstract: PURPOSE: A transgenic Petunia hybrid having resistance to non-biological stress is provided to resistance to non-biological stress such as dryness, low temperature, moisture, and heavy metal or air pollution. CONSTITUTION: A seed of Petunia hybrid(KACC 98002P and KACC 98003P) has resistance to non-biological stress by being inserted with gene of the sequence number 1. The gene is Escherichia coli-derived SOD(superoxide dismutase) gene. A method for producing seed of Petunia hybrid comprises: a step of introducing SOD gene to Pbi121 vector; a step of transforming the vector to Agrobacterium tumefaciens; a step of co-culturing the transformed Agrobacterium with leaves fraction of micropropagated shoot; and a step of confirming SOD gene through van der Meer method.

Abstract translation: 目的：提供抗非生物应激的转基因矮牵牛杂种，以抵抗非生物胁迫，如干燥，低温，潮湿，重金属或空气污染。 构成：通过插入序列号1的基因，矮牵牛杂种（KACC 98002P和KACC 98003P）的种子具有对非生物胁迫的抗性。该基因是大肠杆菌来源的SOD（超氧化物歧化酶）基因。 矮牵牛种子的种子生产方法包括：向Pbi121载体引入SOD基因的步骤; 将载体转化到根癌土壤杆菌的步骤; 与转化的土壤杆菌共同培养具有微繁殖芽的叶部分的步骤; 并通过van der Meer方法确认SOD基因的一个步骤。