레스베리윤문바이러스 진단용 프라이머 조합 및 이의 용도
    1.
    发明公开
    레스베리윤문바이러스 진단용 프라이머 조합 및 이의 용도 有权
    用于诊断RASPBERRY RINGSPOT病毒的PRIMER组合及其用途

    公开(公告)号:KR1020100082962A

    公开(公告)日:2010-07-21

    申请号:KR1020090002284

    申请日:2009-01-12

    CPC classification number: C12N15/10 C12N15/11 C12Q1/6851 C12Q1/6853 C12Q1/701

    Abstract: PURPOSE: A primer combination for raspberry ringspot virus(RpRSV) and a method for diagnosing RpRSV are provided to diagnose with high detection limit. CONSTITUTION: A primer combination for diagnosing RpRSV comprises forward primer and reverse primer. The forward primer is selected from base sequences of sequence numbers 1, 2, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, and 19. The reverse primer is selected from base sequences of sequence numbers 23, 24, 25, 26, 28, 31, 32, 33, 34, 35, 36, 39, and 41. The primer combination for diagnosing RpRSV comprises a primer of sequence numbers 12 and 28, sequence number 11 and 28, or sequence numbers 8 and 31. A method for specifically diagnosing RpRSV comprises: a step of isolating total RNA from a plant sample; a step of amplifying a target seqeuence by RT-PCR; and a step of detecting the PCR product.

    Abstract translation: 目的:提供覆盆子环斑病毒(RpRSV)的引物组合和诊断RpRSV的方法,以高检出限进行诊断。 构成:用于诊断RpRSV的引物组合包括正向引物和反向引物。 正向引物选自序列号1,2,4,5,7,8,9,10,11,12,13,14,15,16,17和19的碱基序列。反向引物选自 序列号23,24,25,26,28,31,32,33,34,35,36,39和41的碱基序列。用于诊断RpRSV的引物组合包含序列号12和28的引物,序列号 11和28,或序列号8和31.用于特异性诊断RpRSV的方法包括:从植物样品分离总RNA的步骤; 通过RT-PCR扩增靶序列的步骤; 以及检测PCR产物的步骤。

    담배래틀바이러스 진단용 프라이머 조합 및 이의 용도
    3.
    发明公开
    담배래틀바이러스 진단용 프라이머 조합 및 이의 용도 有权
    用于诊断烟草瘟疫病毒的主要组合及其用途

    公开(公告)号:KR1020100082975A

    公开(公告)日:2010-07-21

    申请号:KR1020090002301

    申请日:2009-01-12

    CPC classification number: C12N15/10 C12N15/11 C12Q1/6851 C12Q1/6853 C12Q1/701

    Abstract: PURPOSE: A primer set for diagnosing tobacco rattle virus(TRV) and a method for diagnosing TRV using the same are provided to accurately diagnose TRV infection from Solanaceae seed or plant. CONSTITUTION: A primer set for diagnosing TRV comprises a forward primer selected from sequence numbers 1, 2, 3, 4, 5 and 6, and reverse primer selected from sequence numbers 9, 10, 11, 12, 13, 14 and 15. The primer set for diagnosing TRV comprises a primer set of sequence number 3 and 1 and primer set of sequence numbers 1 and 14. A method for diagnosing TRV comprises: a step of isolating total RNA from a plant sample; a step of amplifying a target sequence by RT-PCR; and a step of detecting PCR product.

    Abstract translation: 目的:提供用于诊断烟草拨鼠病毒(TRV)的引物组和使用其的诊断TRV的方法,以准确诊断茄科种子或植物的TRV感染。 构成:用于诊断TRV的引物组包含选自序列号1,2,3,4,5和6的正向引物,以及选自序列号9,10,11,12,13,14和15的反向引物。 用于诊断TRV的引物组包含序列号3和1的引物组和序列号1和14的引物组。诊断TRV的方法包括:从植物样品中分离总RNA的步骤; 通过RT-PCR扩增靶序列的步骤; 以及检测PCR产物的步骤。

    RDV를 진단 또는 검출하기 위한 프로브 세트 및 이의 용도
    10.
    发明授权
    RDV를 진단 또는 검출하기 위한 프로브 세트 및 이의 용도 有权
    用于诊断或检测水稻矮秆病毒及其用途的探针

    公开(公告)号:KR101650564B1

    公开(公告)日:2016-08-24

    申请号:KR1020110117795

    申请日:2011-11-11

    Abstract: 본발명은서열번호 1953 내지 1964 및서열번호 1982 내지 1991의올리고뉴클레오티드및 이의상보적올리고뉴클레오티드로구성되는군으로부터선택되는하나이상의올리고뉴클레오티드를포함하는 RDV (Rice dwarf virus) 올리고뉴클레오티드프로브세트, 상기올리고뉴클레오티드프로브세트를포함하는 RDV 진단또는검출용유전자칩, RDV에감염된것으로추정되는시료로부터 RNA를분리하는단계, 상기분리된 RNA를주형으로하여 cDNA를합성하는단계, 상기합성된 cDNA를상기올리고뉴클레오티드프로브세트와접촉시켜상기 cDNA와프로브세트를혼성화시키는단계및 상기프로브와 cDNA 사이의혼성화정도를검출하는단계를포함하는 RDV를진단또는검출하는방법및 상기올리고뉴클레오티드프로브세트및 혼성화용액을포함하는 RDV는검출용키트에관한것이다.

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