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    담배래틀바이러스 진단용 프라이머 조합 및 이의 용도
    1.
    发明公开
    담배래틀바이러스 진단용 프라이머 조합 및 이의 용도 有权
    用于诊断烟草瘟疫病毒的主要组合及其用途

    公开(公告)号：KR1020100082975A

    公开(公告)日：2010-07-21

    申请号：KR1020090002301

    申请日：2009-01-12

    Applicant: 대한민국(농촌진흥청장) ,  대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 이수헌 ,  신용길 ,  한상진 ,  최홍수 ,  김정수 ,  이영규

    IPC: C12N15/10 ,  C12N15/33 ,  C12Q1/68

    CPC classification number: C12N15/10 ,  C12N15/11 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/701

    Abstract: PURPOSE: A primer set for diagnosing tobacco rattle virus(TRV) and a method for diagnosing TRV using the same are provided to accurately diagnose TRV infection from Solanaceae seed or plant. CONSTITUTION: A primer set for diagnosing TRV comprises a forward primer selected from sequence numbers 1, 2, 3, 4, 5 and 6, and reverse primer selected from sequence numbers 9, 10, 11, 12, 13, 14 and 15. The primer set for diagnosing TRV comprises a primer set of sequence number 3 and 1 and primer set of sequence numbers 1 and 14. A method for diagnosing TRV comprises: a step of isolating total RNA from a plant sample; a step of amplifying a target sequence by RT-PCR; and a step of detecting PCR product.

    Abstract translation: 目的：提供用于诊断烟草拨鼠病毒（TRV）的引物组和使用其的诊断TRV的方法，以准确诊断茄科种子或植物的TRV感染。 构成：用于诊断TRV的引物组包含选自序列号1,2,3,4,5和6的正向引物，以及选自序列号9,10,11,12,13,14和15的反向引物。 用于诊断TRV的引物组包含序列号3和1的引物组和序列号1和14的引物组。诊断TRV的方法包括：从植物样品中分离总RNA的步骤; 通过RT-PCR扩增靶序列的步骤; 以及检测PCR产物的步骤。

    레스베리윤문바이러스 진단용 프라이머 조합 및 이의 용도
    3.
    发明公开
    레스베리윤문바이러스 진단용 프라이머 조합 및 이의 용도 有权
    用于诊断RASPBERRY RINGSPOT病毒的PRIMER组合及其用途

    公开(公告)号：KR1020100082962A

    公开(公告)日：2010-07-21

    申请号：KR1020090002284

    申请日：2009-01-12

    Applicant: 대한민국(농촌진흥청장) ,  대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 이수헌 ,  신용길 ,  한상진 ,  최홍수 ,  김정수 ,  이준성

    IPC: C12N15/10 ,  C12N15/33 ,  C12Q1/68

    CPC classification number: C12N15/10 ,  C12N15/11 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/701

    Abstract: PURPOSE: A primer combination for raspberry ringspot virus(RpRSV) and a method for diagnosing RpRSV are provided to diagnose with high detection limit. CONSTITUTION: A primer combination for diagnosing RpRSV comprises forward primer and reverse primer. The forward primer is selected from base sequences of sequence numbers 1, 2, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, and 19. The reverse primer is selected from base sequences of sequence numbers 23, 24, 25, 26, 28, 31, 32, 33, 34, 35, 36, 39, and 41. The primer combination for diagnosing RpRSV comprises a primer of sequence numbers 12 and 28, sequence number 11 and 28, or sequence numbers 8 and 31. A method for specifically diagnosing RpRSV comprises: a step of isolating total RNA from a plant sample; a step of amplifying a target seqeuence by RT-PCR; and a step of detecting the PCR product.

    Abstract translation: 目的：提供覆盆子环斑病毒（RpRSV）的引物组合和诊断RpRSV的方法，以高检出限进行诊断。 构成：用于诊断RpRSV的引物组合包括正向引物和反向引物。 正向引物选自序列号1,2,4,5,7,8,9,10,11,12,13,14,15,16,17和19的碱基序列。反向引物选自 序列号23,24,25,26,28,31,32,33,34,35,36,39和41的碱基序列。用于诊断RpRSV的引物组合包含序列号12和28的引物，序列号 11和28，或序列号8和31.用于特异性诊断RpRSV的方法包括：从植物样品分离总RNA的步骤; 通过RT-PCR扩增靶序列的步骤; 以及检测PCR产物的步骤。

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