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公开(公告)号：KR100899900B1
公开(公告)日：2009-05-27
申请号：KR1020080128491
申请日：2008-12-17
Applicant: 대한민국 (식품의약품안전처장)
CPC classification number: C12P7/64 , C12Y301/01003
Abstract: 본 발명은 유지의 산화안정성을 개선시키는 방법에 관한 것으로서, 보다 상세하게는 1) 고체 유지와 식물성 유래 액체 유지를 혼합하는 단계; 및 2) 리파아제가 존재하는 연속식 효소 반응기(PBR: Packed Bed Reactor)에서 상기 혼합유를 70℃에서 24∼60분 동안 머무르게 하여 반응시키는 효소적 에스테르교환반응(EI: Enzymatic Interesterification)을 수행하는 단계를 거쳐 유지의 산화안정성을 개선시키는 방법에 관한 것이다.
고체 유지, 식물성 유래 액체 유지, 리파아제, 에스테르교환반응, 과산화물가, 아니시딘가, 공액 이중산 함량, 산화안정성-
公开(公告)号：KR100862548B1
公开(公告)日：2008-10-13
申请号：KR1020070132998
申请日：2007-12-18
Applicant: 대한민국 (식품의약품안전처장)
CPC classification number: A23D9/02 , C11C3/10 , C12Y301/01003
Abstract: A method of manufacturing trans fat-free oils is provided to enable the use of enzymes for a long time by increasing residual activity of enzymes and to reduce energy cost, refining loss by reducing by-products such as free fatty acid, monoglyceride and diglyceride and the formation of free radicals. Trans fat-free oils are prepared by the steps of: mixing extremely hydrogenated oil derived from canola oil, and olive oil in a weight ratio of 1:8 to 9:1; adding lipase to the mixed oil and reacting at 65 to 80deg.C for 1 to 4hr in a first step and at 40 to 60deg.C for 44 to 47hr in a second step. The lipase is one or more selected from lipase derived from microorganisms containing Rhizopus delemar, Mucor miehei, Alcaligenes sp., Aspergillus niger, Candida antarctica, Candida cylindracea and Geotrichum candidum; lipase derived from plants containing soybean; and animal pancreatic lipase.
Abstract translation: 提供了制造反式无脂肪油的方法，以通过增加酶的残留活性并降低能量成本，通过减少副产物如游离脂肪酸，甘油单酯和甘油二酯来提炼损失，从而长时间地使用酶，以及 形成自由基。 通过以下步骤制备反式无脂肪油：将来自低芥酸菜子油的非常氢化的油和橄榄油以1:8至9:1的重量比混合; 向混合油中加入脂肪酶，在第一步中在65〜80℃下反应1〜4小时，在第二步中在40〜60℃下反应44〜47小时。 所述脂肪酶是选自源自含有根霉属，Mucor miehei，Alcaligenes sp。，黑曲霉，南极假丝酵母，圆瓶假丝酵母和地衣芽孢杆菌的微生物的脂肪酶中的一种或多种; 源自含大豆植物的脂肪酶; 和动物胰脂肪酶。
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3.发明授权저 포화, 무 트랜스 지방 함유 유지를 개발하기 위한 단계별 온도 시스템에서의 연속식 효소적 에스테르교환반응 有权连续包装床反应器中的酶活性在温度范围内用于开发无脂肪和低饱和脂肪的脂肪和油脂
公开(公告)号：KR100903705B1
公开(公告)日：2009-06-19
申请号：KR1020080128490
申请日：2008-12-17
Applicant: 대한민국 (식품의약품안전처장)
Abstract: Continuous enzymatic interesterification in a stepwise temperature changing system is provided to produce oils and fats containing less than 30% of saturated fat without trans fats by reacting to mixed oil of soybean oil-derived extremely hardened oils and high-oleic acid sunflower oils at higher temperature than a melting point of the mixed oil and re-reacting to the mixed oil at lower temperature than the melting point of the mixed oil. A method for preparing oils and fats containing less than 30% of saturated fat comprises the following steps of: mixing soybean oil-derived extremely hardened oils and high-oleic acid sunflower oils; primarily reacting to the mixed oil in a continuous packed-bed reactor with lipase at 70°C for 3-15 minutes; and secondarily reacting to the mixed in the continuous packed-bed reactor with lipase at 60°C for 3-200 minutes. The mixing ratio of the soybean oil-derived extremely hardened oils and high-oleic acid sunflower oil is 1 to 9 or 9 to 1. The lipase is selected from the group consisting of: lipase derived from microorganism containing Rhizopus delemar, Mucor miehei, Alcaligenes sp., Aspergillus niger, Candida Antarctica, Candida cylindracea and Geotrichum candidum; lipase derived from plant containing seeds from soybean, manuka and castor bean; and animal pancreatic lipase.
Abstract translation: 提供逐步温度变化系统中的连续酶促酯化以通过与大豆油衍生的极硬化油和高油酸葵花籽油在较高温度下的混合油反应而产生含有少于30％的饱和脂肪而不反式脂肪的油和脂肪 比混合油的熔点高，并且在比混合油的熔点低的温度下与混合油重新反应。 制备含有少于30％饱和脂肪的油和脂肪的方法包括以下步骤：混合大豆油衍生的极硬化油和高油酸葵花籽油; 在70℃的脂肪酶连续填充床反应器中主要与混合油反应3-15分钟; 并在连续填充床反应器中使其与60℃的脂肪酶混合反应3-200分钟。 大豆油衍生的极硬化油和高油酸向日葵油的混合比例为1〜9或9比1。脂肪酶选自：来源于含有根霉属的微生物的脂肪酶，Mechor miehei，产碱杆菌 黑曲霉，南极假丝酵母，圆珠假丝酵母和地衣芽孢杆菌; 来自含有大豆，麦芽糖和蓖麻籽的植物的脂肪酶; 和动物胰脂肪酶。
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