Abstract:
Continuous enzymatic interesterification in a stepwise temperature changing system is provided to produce oils and fats containing less than 30% of saturated fat without trans fats by reacting to mixed oil of soybean oil-derived extremely hardened oils and high-oleic acid sunflower oils at higher temperature than a melting point of the mixed oil and re-reacting to the mixed oil at lower temperature than the melting point of the mixed oil. A method for preparing oils and fats containing less than 30% of saturated fat comprises the following steps of: mixing soybean oil-derived extremely hardened oils and high-oleic acid sunflower oils; primarily reacting to the mixed oil in a continuous packed-bed reactor with lipase at 70°C for 3-15 minutes; and secondarily reacting to the mixed in the continuous packed-bed reactor with lipase at 60°C for 3-200 minutes. The mixing ratio of the soybean oil-derived extremely hardened oils and high-oleic acid sunflower oil is 1 to 9 or 9 to 1. The lipase is selected from the group consisting of: lipase derived from microorganism containing Rhizopus delemar, Mucor miehei, Alcaligenes sp., Aspergillus niger, Candida Antarctica, Candida cylindracea and Geotrichum candidum; lipase derived from plant containing seeds from soybean, manuka and castor bean; and animal pancreatic lipase.
Abstract:
본 발명은 유지의 산화안정성을 개선시키는 방법에 관한 것으로서, 보다 상세하게는 1) 고체 유지와 식물성 유래 액체 유지를 혼합하는 단계; 및 2) 리파아제가 존재하는 연속식 효소 반응기(PBR: Packed Bed Reactor)에서 상기 혼합유를 70℃에서 24∼60분 동안 머무르게 하여 반응시키는 효소적 에스테르교환반응(EI: Enzymatic Interesterification)을 수행하는 단계를 거쳐 유지의 산화안정성을 개선시키는 방법에 관한 것이다. 고체 유지, 식물성 유래 액체 유지, 리파아제, 에스테르교환반응, 과산화물가, 아니시딘가, 공액 이중산 함량, 산화안정성