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公开(公告)号：KR100933223B1
公开(公告)日：2009-12-22
申请号：KR1020060112125
申请日：2006-11-14
Applicant: 대한민국 (식품의약품안전처장)
IPC: C12Q1/68
Abstract: 본 발명은 한우 판별법에 관한 것이다. 보다 상세하게는, 본 발명은 한우와 수입육을 판별할 수 있는 SNP 마커 개발에 관한 것이다. 이를 위해 NCBI Bovine SNP DB 및 AFNS Bovine SNP DB에서 SNP를 선발하여 한우 및 수입육에서 지노타이핑 (genotyping)을 실시하였다. 3,072 SNP 가운데 2,663개가 성공적으로 지노타이핑 되었으며 한우와 수입우종간에 MAF (minor allele frequency) 차이가 0.35 이상인 SNP 총 192개를 확보하고, 이로부터 70개 마커 세트 (set)를 최종 구성하였다. 개발된 마커 세트를 1차 실증성 검증하였으며, 2차로 블라인드 테스트 (blind test)를 통해 실증성을 검증한 결과 한우와 수입육을 모두 정확히 판별할 수 있음을 확인하였다. 따라서, 본 발명의 70개 마커 세트를 포함하는 한우 판별법은 국내에서 유통되고 있는 수입육과 한우의 판별용으로 적합하다.
한우, 판별, SNP, 마커Abstract translation: 通过SNP（单核苷酸多态性）标记，准确区分韩牛（韩国牛）与进口牛的差异，改善韩窝，奶牛，家养牛和进口牛的混合循环结构，通过保护基因来维持和促进韩宇的生产基础 的韩宇，并确定韩宇的阶级。 从SEQ ID NO：1到SEQ ID NO：70的核苷酸序列中选择用于区分Hanwoo和进口牛的SNP标记。 将韩宇与进口牛区分的方法包括以下步骤：（i）从待检牛的DNA中提取DNA; （ii）扩增含有SEQ ID NO：1至SEQ ID NO：70的任何一个碱基突变位点或其互补序列的DNA; （iii）合成与SEQ ID NO：1至SEQ ID NO：70的任一个碱基突变位点的核苷酸序列互补的引物或与其互补序列含有与其相邻的一个碱基位点的互补序列; （iv）通过使用测序仪确定步骤（iii）的反应中使用的碱基种类; 和（v）将步骤（iv）中确定的碱基种类与对照进行比较。
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公开(公告)号：KR100382172B1
公开(公告)日：2003-05-01
申请号：KR1020000076487
申请日：2000-12-14
Applicant: 대한민국 (식품의약품안전처장)
IPC: G01N33/12
Abstract: PURPOSE: A method is provided to establish order of commercial dealings by discriminating natural yellow croakers from counterfeit yellow croakers in an easy and convenient manner. CONSTITUTION: A method comprises the steps of dissolving pigment from the colored portion by using water; and judging whether the pigment is decolorized from the colored portion by using CH3CO3H. Alternatively, a method comprises the steps of dissolving pigment from the colored portion by using water; and dissolving pigment from the colored portion by using acetone. Alternatively, a method comprises the steps of dissolving pigment from the colored portion by using water; judging whether the pigment is decolorized from the colored portion by using CH3CO3H; and dissolving pigment from the colored portion by using acetone.
Abstract translation: 目的：提供一种方法，通过简单方便的方式区分天然黄鱼与假黄鱼，从而确定商业交易的顺序。 组成：一种方法包括以下步骤：通过使用水从着色部分溶解颜料; 并判断颜料是否使用CH 3 CO 3 H从着色部分脱色。 或者，一种方法包括以下步骤：通过使用水从颜色部分溶解颜料; 并通过使用丙酮从着色部分溶解颜料。 或者，一种方法包括以下步骤：通过使用水从颜色部分溶解颜料; 判断颜料是否使用CH 3 CO 3 H从着色部分脱色; 并通过使用丙酮从着色部分溶解颜料。
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公开(公告)号：KR1020020046417A
公开(公告)日：2002-06-21
申请号：KR1020000076487
申请日：2000-12-14
Applicant: 대한민국 (식품의약품안전처장)
IPC: G01N33/12
Abstract: PURPOSE: A method is provided to establish order of commercial dealings by discriminating natural yellow croakers from counterfeit yellow croakers in an easy and convenient manner. CONSTITUTION: A method comprises the steps of dissolving pigment from the colored portion by using water; and judging whether the pigment is decolorized from the colored portion by using CH3CO3H. Alternatively, a method comprises the steps of dissolving pigment from the colored portion by using water; and dissolving pigment from the colored portion by using acetone. Alternatively, a method comprises the steps of dissolving pigment from the colored portion by using water; judging whether the pigment is decolorized from the colored portion by using CH3CO3H; and dissolving pigment from the colored portion by using acetone.
Abstract translation: 目的：提供一种方法，通过以简单方便的方式区分天然黄鱼与假冒黄鱼，建立商业交易秩序。 构成：一种方法包括通过使用水从着色部分溶解颜料的步骤; 并使用CH3CO3H判断颜料是否从着色部分脱色。 或者，一种方法包括通过使用水从着色部分溶解颜料的步骤; 并通过使用丙酮从着色部分溶解颜料。 或者，一种方法包括通过使用水从着色部分溶解颜料的步骤; 通过使用CH3CO3H判断颜料是否从着色部分脱色; 并通过使用丙酮从着色部分溶解颜料。
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公开(公告)号：KR100862548B1
公开(公告)日：2008-10-13
申请号：KR1020070132998
申请日：2007-12-18
Applicant: 대한민국 (식품의약품안전처장)
CPC classification number: A23D9/02 , C11C3/10 , C12Y301/01003
Abstract: A method of manufacturing trans fat-free oils is provided to enable the use of enzymes for a long time by increasing residual activity of enzymes and to reduce energy cost, refining loss by reducing by-products such as free fatty acid, monoglyceride and diglyceride and the formation of free radicals. Trans fat-free oils are prepared by the steps of: mixing extremely hydrogenated oil derived from canola oil, and olive oil in a weight ratio of 1:8 to 9:1; adding lipase to the mixed oil and reacting at 65 to 80deg.C for 1 to 4hr in a first step and at 40 to 60deg.C for 44 to 47hr in a second step. The lipase is one or more selected from lipase derived from microorganisms containing Rhizopus delemar, Mucor miehei, Alcaligenes sp., Aspergillus niger, Candida antarctica, Candida cylindracea and Geotrichum candidum; lipase derived from plants containing soybean; and animal pancreatic lipase.
Abstract translation: 提供了制造反式无脂肪油的方法，以通过增加酶的残留活性并降低能量成本，通过减少副产物如游离脂肪酸，甘油单酯和甘油二酯来提炼损失，从而长时间地使用酶，以及 形成自由基。 通过以下步骤制备反式无脂肪油：将来自低芥酸菜子油的非常氢化的油和橄榄油以1:8至9:1的重量比混合; 向混合油中加入脂肪酶，在第一步中在65〜80℃下反应1〜4小时，在第二步中在40〜60℃下反应44〜47小时。 所述脂肪酶是选自源自含有根霉属，Mucor miehei，Alcaligenes sp。，黑曲霉，南极假丝酵母，圆瓶假丝酵母和地衣芽孢杆菌的微生物的脂肪酶中的一种或多种; 源自含大豆植物的脂肪酶; 和动物胰脂肪酶。
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Patent Agency Ranking
公开(公告)号：KR1020100019682A
公开(公告)日：2010-02-19
申请号：KR1020080078369
申请日：2008-08-11
Applicant: 대한민국 (식품의약품안전처장)
IPC: G06Q50/10 , G06F3/0484
CPC classification number: G06Q50/10 , G06F3/04845
Abstract: PURPOSE: A server for food menu preparation and nutrition valuation and a method thereof are provided to make out various menus by preparing a menu for production registered by a user. CONSTITUTION: A detailed item of menu information is inputted(S410), and a menu marking screen is activated(S415). On the menu writing screen, a meal is selected(S420). At least one of a recommended menu and a user menu is displayed(S430). When a specific menu is selected, the selected menu is added to a menu list for the meal(S445,S447). The nutrition for the made menu is evaluated(S455).
Abstract translation: 目的：提供用于食物菜单准备和营养评估的服务器及其方法，以通过准备用户登记的生产菜单来制作各种菜单。 构成：输入菜单信息的详细项目（S410），菜单标记画面被激活（S415）。 在菜单写入屏幕上，选择餐（S420）。 显示推荐菜单和用户菜单中的至少一个（S430）。 当选择特定菜单时，所选择的菜单被添加到菜单菜单中（S445，S447）。 对菜单进行营养评估（S455）。
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公开(公告)号：KR100899900B1
公开(公告)日：2009-05-27
申请号：KR1020080128491
申请日：2008-12-17
Applicant: 대한민국 (식품의약품안전처장)
CPC classification number: C12P7/64 , C12Y301/01003
Abstract: 본 발명은 유지의 산화안정성을 개선시키는 방법에 관한 것으로서, 보다 상세하게는 1) 고체 유지와 식물성 유래 액체 유지를 혼합하는 단계; 및 2) 리파아제가 존재하는 연속식 효소 반응기(PBR: Packed Bed Reactor)에서 상기 혼합유를 70℃에서 24∼60분 동안 머무르게 하여 반응시키는 효소적 에스테르교환반응(EI: Enzymatic Interesterification)을 수행하는 단계를 거쳐 유지의 산화안정성을 개선시키는 방법에 관한 것이다.
고체 유지, 식물성 유래 액체 유지, 리파아제, 에스테르교환반응, 과산화물가, 아니시딘가, 공액 이중산 함량, 산화안정성-
公开(公告)号：KR1020080043494A
公开(公告)日：2008-05-19
申请号：KR1020060112125
申请日：2006-11-14
Applicant: 대한민국 (식품의약품안전처장)
IPC: C12Q1/68
CPC classification number: C12Q1/6827 , C12Q2600/156
Abstract: SNP(single nucleotide polymorphism) markers are provided to discriminate Hanwoo(Korean cattle) from importing cattle accurately, improve the mixed circulation structure of Hanwoo, milk cow, domestic cattle and importing cattle, and maintain and promote production foundation of Hanwoo by protecting gene sources of Hanwoo and identifying classes of Hanwoo. An SNP marker for discriminating Hanwoo from importing cattle is selected from the nucleotide sequences of SEQ ID NO:1 to SEQ ID NO:70. A method for discriminating Hanwoo from importing cattle comprises the steps of: (i) extracting DNA from a cattle to be examined; (ii) amplifying the DNA containing any one base mutation site of SEQ ID NO:1 to SEQ ID NO:70 or a complementary sequence therewith; (iii) synthesizing a primer complementary with the nucleotide sequence of any one base mutation site of SEQ ID NO:1 to SEQ ID NO:70 or with the complementary sequence therewith containing one base site adjacent thereto; (iv) determining the base kind used in the reaction of step (iii) by using a sequencer; and (v) comparing the base kind determined in step (iv) with a control.
Abstract translation: 提供SNP（单核苷酸多态性）标记，以准确鉴别汉诺牛（韩国牛）进口牛，改善Hanwoo，奶牛，家畜牛和进口牛的混合循环结构，保护和促进Hanwoo的生产基础，保护基因来源 的Hanwoo，并确定Hanwoo的课程。 从SEQ ID NO：1至SEQ ID NO：70的核苷酸序列中选择用于鉴别Hanwoo与进口牛的SNP标记。 一种鉴别Hanwoo与进口牛的方法包括以下步骤：（i）从待检牛的DNA中提取DNA; （ii）扩增含有SEQ ID NO：1至SEQ ID NO：70的任一个碱基突变位点的DNA或其互补序列; （iii）合成与SEQ ID NO：1至SEQ ID NO：70的任一个碱基突变位点的核苷酸序列互补的引物或与其相邻的一个碱基位点的互补序列互补的引物; （iv）使用定序器确定步骤（iii）的反应中使用的碱基类型; 和（v）将步骤（iv）中确定的碱基类型与对照相比较。
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8.发明授权저 포화, 무 트랜스 지방 함유 유지를 개발하기 위한 단계별 온도 시스템에서의 연속식 효소적 에스테르교환반응 有权连续包装床反应器中的酶活性在温度范围内用于开发无脂肪和低饱和脂肪的脂肪和油脂
公开(公告)号：KR100903705B1
公开(公告)日：2009-06-19
申请号：KR1020080128490
申请日：2008-12-17
Applicant: 대한민국 (식품의약품안전처장)
Abstract: Continuous enzymatic interesterification in a stepwise temperature changing system is provided to produce oils and fats containing less than 30% of saturated fat without trans fats by reacting to mixed oil of soybean oil-derived extremely hardened oils and high-oleic acid sunflower oils at higher temperature than a melting point of the mixed oil and re-reacting to the mixed oil at lower temperature than the melting point of the mixed oil. A method for preparing oils and fats containing less than 30% of saturated fat comprises the following steps of: mixing soybean oil-derived extremely hardened oils and high-oleic acid sunflower oils; primarily reacting to the mixed oil in a continuous packed-bed reactor with lipase at 70°C for 3-15 minutes; and secondarily reacting to the mixed in the continuous packed-bed reactor with lipase at 60°C for 3-200 minutes. The mixing ratio of the soybean oil-derived extremely hardened oils and high-oleic acid sunflower oil is 1 to 9 or 9 to 1. The lipase is selected from the group consisting of: lipase derived from microorganism containing Rhizopus delemar, Mucor miehei, Alcaligenes sp., Aspergillus niger, Candida Antarctica, Candida cylindracea and Geotrichum candidum; lipase derived from plant containing seeds from soybean, manuka and castor bean; and animal pancreatic lipase.
Abstract translation: 提供逐步温度变化系统中的连续酶促酯化以通过与大豆油衍生的极硬化油和高油酸葵花籽油在较高温度下的混合油反应而产生含有少于30％的饱和脂肪而不反式脂肪的油和脂肪 比混合油的熔点高，并且在比混合油的熔点低的温度下与混合油重新反应。 制备含有少于30％饱和脂肪的油和脂肪的方法包括以下步骤：混合大豆油衍生的极硬化油和高油酸葵花籽油; 在70℃的脂肪酶连续填充床反应器中主要与混合油反应3-15分钟; 并在连续填充床反应器中使其与60℃的脂肪酶混合反应3-200分钟。 大豆油衍生的极硬化油和高油酸向日葵油的混合比例为1〜9或9比1。脂肪酶选自：来源于含有根霉属的微生物的脂肪酶，Mechor miehei，产碱杆菌 黑曲霉，南极假丝酵母，圆珠假丝酵母和地衣芽孢杆菌; 来自含有大豆，麦芽糖和蓖麻籽的植物的脂肪酶; 和动物胰脂肪酶。
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公开(公告)号：KR100816738B1
公开(公告)日：2008-03-25
申请号：KR1020070014617
申请日：2007-02-13
Applicant: 대한민국 (식품의약품안전처장)
IPC: C12Q1/68
CPC classification number: C12Q1/6844 , C12Q1/6853
Abstract: A primer set for detecting an insect-resistant rice is provided to be used for detecting a Cry1Ac including insect-resistant rice(Cry1Ac-Bt rice) simply and rapidly, thereby controlling and managing illegal inflow of the Cry1Ac-Bt rice unauthorized by our country. A primer set for detecting an insect-resistant rice comprises primers described as SEQ ID : NOs. 1 to 4, wherein the insect-resistant rice includes a Cry1Ac gene coding an insect-resistant protein. A method for detecting the insect-resistant rice comprising the Cry1Ac comprises a step of subjecting the rice to a polymerase chain reaction(PCR) using the primers. Further, the insect-resistant rice comprising the Cry1Ac comprises cry1Ac gene coding an insect-resistant protein.
Abstract translation: 提供了一种用于检测抗虫水稻的引物组，用于快速检测包括抗虫稻（Cry1Ac-Bt稻）的Cry1Ac，从而控制和管理我国未经许可的Cry1Ac-Bt大米的非法流入 。 用于检测抗虫水稻的引物组包括如SEQ ID NO：所示的引物。 如图1至图4所示，其中抗虫性水稻包括编码昆虫抗性蛋白质的Cry1Ac基因。 检测包含Cry1Ac的抗虫水稻的方法包括使用引物对稻进行聚合酶链式反应（PCR）的步骤。 此外，包含Cry1Ac的抗虫水稻包含编码昆虫抗性蛋白质的cry1Ac基因。
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