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公开(公告)号：KR100933223B1
公开(公告)日：2009-12-22
申请号：KR1020060112125
申请日：2006-11-14
Applicant: 대한민국 (식품의약품안전처장)
IPC: C12Q1/68
Abstract: 본 발명은 한우 판별법에 관한 것이다. 보다 상세하게는, 본 발명은 한우와 수입육을 판별할 수 있는 SNP 마커 개발에 관한 것이다. 이를 위해 NCBI Bovine SNP DB 및 AFNS Bovine SNP DB에서 SNP를 선발하여 한우 및 수입육에서 지노타이핑 (genotyping)을 실시하였다. 3,072 SNP 가운데 2,663개가 성공적으로 지노타이핑 되었으며 한우와 수입우종간에 MAF (minor allele frequency) 차이가 0.35 이상인 SNP 총 192개를 확보하고, 이로부터 70개 마커 세트 (set)를 최종 구성하였다. 개발된 마커 세트를 1차 실증성 검증하였으며, 2차로 블라인드 테스트 (blind test)를 통해 실증성을 검증한 결과 한우와 수입육을 모두 정확히 판별할 수 있음을 확인하였다. 따라서, 본 발명의 70개 마커 세트를 포함하는 한우 판별법은 국내에서 유통되고 있는 수입육과 한우의 판별용으로 적합하다.
한우, 판별, SNP, 마커Abstract translation: 通过SNP（单核苷酸多态性）标记，准确区分韩牛（韩国牛）与进口牛的差异，改善韩窝，奶牛，家养牛和进口牛的混合循环结构，通过保护基因来维持和促进韩宇的生产基础 的韩宇，并确定韩宇的阶级。 从SEQ ID NO：1到SEQ ID NO：70的核苷酸序列中选择用于区分Hanwoo和进口牛的SNP标记。 将韩宇与进口牛区分的方法包括以下步骤：（i）从待检牛的DNA中提取DNA; （ii）扩增含有SEQ ID NO：1至SEQ ID NO：70的任何一个碱基突变位点或其互补序列的DNA; （iii）合成与SEQ ID NO：1至SEQ ID NO：70的任一个碱基突变位点的核苷酸序列互补的引物或与其互补序列含有与其相邻的一个碱基位点的互补序列; （iv）通过使用测序仪确定步骤（iii）的反应中使用的碱基种类; 和（v）将步骤（iv）中确定的碱基种类与对照进行比较。
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公开(公告)号：KR1020080043494A
公开(公告)日：2008-05-19
申请号：KR1020060112125
申请日：2006-11-14
Applicant: 대한민국 (식품의약품안전처장)
IPC: C12Q1/68
CPC classification number: C12Q1/6827 , C12Q2600/156
Abstract: SNP(single nucleotide polymorphism) markers are provided to discriminate Hanwoo(Korean cattle) from importing cattle accurately, improve the mixed circulation structure of Hanwoo, milk cow, domestic cattle and importing cattle, and maintain and promote production foundation of Hanwoo by protecting gene sources of Hanwoo and identifying classes of Hanwoo. An SNP marker for discriminating Hanwoo from importing cattle is selected from the nucleotide sequences of SEQ ID NO:1 to SEQ ID NO:70. A method for discriminating Hanwoo from importing cattle comprises the steps of: (i) extracting DNA from a cattle to be examined; (ii) amplifying the DNA containing any one base mutation site of SEQ ID NO:1 to SEQ ID NO:70 or a complementary sequence therewith; (iii) synthesizing a primer complementary with the nucleotide sequence of any one base mutation site of SEQ ID NO:1 to SEQ ID NO:70 or with the complementary sequence therewith containing one base site adjacent thereto; (iv) determining the base kind used in the reaction of step (iii) by using a sequencer; and (v) comparing the base kind determined in step (iv) with a control.
Abstract translation: 提供SNP（单核苷酸多态性）标记，以准确鉴别汉诺牛（韩国牛）进口牛，改善Hanwoo，奶牛，家畜牛和进口牛的混合循环结构，保护和促进Hanwoo的生产基础，保护基因来源 的Hanwoo，并确定Hanwoo的课程。 从SEQ ID NO：1至SEQ ID NO：70的核苷酸序列中选择用于鉴别Hanwoo与进口牛的SNP标记。 一种鉴别Hanwoo与进口牛的方法包括以下步骤：（i）从待检牛的DNA中提取DNA; （ii）扩增含有SEQ ID NO：1至SEQ ID NO：70的任一个碱基突变位点的DNA或其互补序列; （iii）合成与SEQ ID NO：1至SEQ ID NO：70的任一个碱基突变位点的核苷酸序列互补的引物或与其相邻的一个碱基位点的互补序列互补的引物; （iv）使用定序器确定步骤（iii）的反应中使用的碱基类型; 和（v）将步骤（iv）中确定的碱基类型与对照相比较。
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公开(公告)号：KR100816738B1
公开(公告)日：2008-03-25
申请号：KR1020070014617
申请日：2007-02-13
Applicant: 대한민국 (식품의약품안전처장)
IPC: C12Q1/68
CPC classification number: C12Q1/6844 , C12Q1/6853
Abstract: A primer set for detecting an insect-resistant rice is provided to be used for detecting a Cry1Ac including insect-resistant rice(Cry1Ac-Bt rice) simply and rapidly, thereby controlling and managing illegal inflow of the Cry1Ac-Bt rice unauthorized by our country. A primer set for detecting an insect-resistant rice comprises primers described as SEQ ID : NOs. 1 to 4, wherein the insect-resistant rice includes a Cry1Ac gene coding an insect-resistant protein. A method for detecting the insect-resistant rice comprising the Cry1Ac comprises a step of subjecting the rice to a polymerase chain reaction(PCR) using the primers. Further, the insect-resistant rice comprising the Cry1Ac comprises cry1Ac gene coding an insect-resistant protein.
Abstract translation: 提供了一种用于检测抗虫水稻的引物组，用于快速检测包括抗虫稻（Cry1Ac-Bt稻）的Cry1Ac，从而控制和管理我国未经许可的Cry1Ac-Bt大米的非法流入 。 用于检测抗虫水稻的引物组包括如SEQ ID NO：所示的引物。 如图1至图4所示，其中抗虫性水稻包括编码昆虫抗性蛋白质的Cry1Ac基因。 检测包含Cry1Ac的抗虫水稻的方法包括使用引物对稻进行聚合酶链式反应（PCR）的步骤。 此外，包含Cry1Ac的抗虫水稻包含编码昆虫抗性蛋白质的cry1Ac基因。
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