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公开(公告)号:KR101277801B1
公开(公告)日:2013-06-27
申请号:KR1020120059418
申请日:2012-06-01
Applicant: 대한민국 (식품의약품안전처장)
Abstract: PURPOSE: A method for determining the mixture of a root vegetable ingredient using a primer set containing a forward primer and a reverse primer is provided to scientifically monitor foods, and to prevent distribution of bad foods. CONSTITUTION: A method for determining the mixture of a root vegetable ingredient comprises: a step of isolating DNA from a food ingredient; a step of performing first, second, third, and fourth PCR using a primer set; and a step of detecting the presence of the root vegetable ingredient including Panax schinseng, Nopsis lanceolata, Platycodon grandiflorum, and Dioscorea batatas. The primer set is selected from the group consisting of: a primer set comprising a forward primer in sequence number 1 and a reverse primer in sequence number 2 for detecting Panax schinseng; a primer set comprising a forward primer in sequence number 3 and a reverse primer in sequence number 4 for detecting Nopsis lanceolata; a primer set comprising a forward primer in sequence number 5 and a reverse primer in sequence number 6 for detecting Platycodon grandiflorum; and a PCR primer comprising a forward primer in sequence number 7 and a reverse primer in sequence number 8 for detecting Dioscorea batatas.
Abstract translation: 目的:提供一种使用含有正向引物和反向引物的引物组确定根植物成分混合物的方法,以科学监测食物,并防止坏食物的分布。 构成:确定根植物成分的混合物的方法包括:从食品成分中分离DNA的步骤; 使用引物组进行第一,第二,第三和第四PCR的步骤; 以及检测包括人参,Nopsis lanceolata,Platycodon grandiflorum和薯a薯等根系植物成分的存在的步骤。 引物组选自:包含序列号1的正向引物的引物组和序列号2的用于检测人参的反向引物; 包含序列号3的正向引物和序列号4的反向引物的引物组,用于检测花椒(Nopsis lanceolata); 一种引物组,其包含序列号5的正向引物和序列号6中用于检测桔梗的反向引物; 和包含序列号7的正向引物和序列号8的反向引物的PCR引物,用于检测薯detecting皂甙。
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公开(公告)号:KR101277800B1
公开(公告)日:2013-06-27
申请号:KR1020120059417
申请日:2012-06-01
Applicant: 대한민국 (식품의약품안전처장)
Abstract: PURPOSE: A technique for determining the mixture of a mollusks ingredient in a food using a primer set containing a forward primer of a certain sequence and a reverse primer of a certain sequence is provided to enable scientific monitoring of a food, and to prevent distribution of bad food. CONSTITUTION: A method for determining the mixture of a mollusks ingredient in a food comprises: a step of isolating DNA from the food; a step of performing first and second PCR using a primer set and DNA as a template; and a step of detecting the presence of the mollusks ingredient in the food. The primer set is selected from the group consisting of: a primer set containing a forward primer in sequence number 1 and a reverse primer in sequence number 2 for detecting Todarodes pacificus; and a primer set containing a forward primer in sequence number 3 and a reverse primer in sequence number 4 for detecting Loligo bleekeri.
Abstract translation: 目的:提供一种用于使用含有某一序列的正向引物和一定序列的反向引物的引物组确定食品中软体动物成分的混合物的技术,以使科学监测食物,并防止 不好的食物。 构成:确定食品中软体动物成分的混合物的方法包括:从食物中分离DNA的步骤; 使用引物组和DNA作为模板进行第一和第二PCR的步骤; 以及检测食品中软体动物成分的存在的步骤。 引物组选自:包含序列号1的正向引物的引物组和序列号为2的反向引物,用于检测太平洋假丝酵母; 和含有序列号3的正向引物和序列号4的反向引物的引物组,用于检测Loligo bleekeri。
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公开(公告)号:KR101277799B1
公开(公告)日:2013-06-27
申请号:KR1020120059415
申请日:2012-06-01
Applicant: 대한민국 (식품의약품안전처장)
Abstract: PURPOSE: A method for determining the mixture of a poultry ingredient in a food using a primer set comprising a forward primer of a certain sequence and a reverse primer of a certain sequence is provided to scientifically monitor foods for prevent distribution of bad foods. CONSTITUTION: A method for determining the mixture of a poultry ingredient in a food comprises: a step of isolating DNA; a step of performing first, second, third, and fourth PCR using a primer set and the DNA as a template; and a step of detecting the poultry ingredient. The primer set is selected from the group consisting of: a primer set comprising a forward primer in sequence number 1 and a reverse primer in sequence number 2 for detecting Gallus gallus; a primer set comprising a forward primer in sequence number 3 and a reverse primer in sequence number 4 for detecting Anas platyrhynchos; a primer set comprising a forward primer in sequence number 5 and a reverse primer in sequence number 6 for detecting Meleagris gallopago; and a primer comprising a forward primer in sequence number 7 and a reverse primer in sequence number 8 for detecting Struthio camelus.
Abstract translation: 目的:提供一种使用包含某一序列的正向引物和一定序列的反向引物的引物组确定食品中家禽成分的混合物的方法,以科学监测食物以防止坏食物的分布。 构成:确定食品中家禽成分混合物的方法包括:分离DNA的步骤; 使用引物组和DNA作为模板进行第一,第二,第三和第四PCR的步骤; 以及检测家禽成分的步骤。 引物组选自:引物组,其包含序列号1的正向引物和序列号为2的反向引物,用于检测Gallus gallus; 包含序列号3的正向引物和序列号4的反向引物的引物组,用于检测Anas platyrhynchos; 序列号5的正向引物和序列号6的反向引物的引物组,用于检测Meleagris gallopago; 和包含序列号7的正向引物和序列号8的反向引物的引物,用于检测骆驼属。
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公开(公告)号:KR101277798B1
公开(公告)日:2013-07-09
申请号:KR1020120059414
申请日:2012-06-01
Applicant: 대한민국 (식품의약품안전처장)
Abstract: PURPOSE: A method for identifying the mixture of meat ingredients in a food ingredient is provided to enable scientific food monitoring and to block distribution of bad foods. CONSTITUTION: A method for identifying the mixture of meat ingredients in a food ingredient comprises: a step of extracting DNA from the food ingredient; a step of performing PCR comprising first, second, third, fourth, fifth, and sixth steps using the DNA and a primer set; and a step of detecting the presence of meats including bos taurus, sus scrofa, ovis aries, capra hircus, cervus elaphus, cervus Nippon, and equus caballus.
Abstract translation: 目的:提供一种鉴定食品中肉类成分混合物的方法,以便科学的食品监测和阻止不良食品的分销。 构成:用于鉴定食品成分中的肉成分混合物的方法包括:从食品成分中提取DNA的步骤; 使用DNA和引物组进行包含第一,第二,第三,第四,第五和第六步骤的PCR的步骤; 检测肉骨的存在的步骤,包括金牛座,苏格兰黑猩猩,奥们白俄罗斯,披肩,鹿耳鹿,日本母鹿和马鞍马。
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公开(公告)号:KR101219763B1
公开(公告)日:2013-01-24
申请号:KR1020120059416
申请日:2012-06-01
Applicant: 대한민국 (식품의약품안전처장)
Abstract: PURPOSE: A method for determining the mixture of a fish ingredient in a food ingredient using a PCR prime set is provided to scientifically monitor foods, and to distribute and manage safety foods. CONSTITUTION: A method for determining the mixture of Oreochromis niloticus in a food ingredient comprises a step of using a PCR primer set containing a forward primer of sequence number 7 and a reverse primer of sequence number 8. The PCR primer set for determining the mixture contains the forward and reverse primers.
Abstract translation: 目的:提供一种使用PCR引物确定食品成分中鱼成分混合物的方法,以科学监测食物,并分发和管理安全食品。 构成:用于测定食品成分中的Oreochromis niloticus的混合物的方法包括使用含有序列号7的正向引物和序列号8的反向引物的PCR引物组的步骤。用于测定混合物的PCR引物组含有 正向和反向引物。
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