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公开(公告)号：WO2014081265A1
公开(公告)日：2014-05-30
申请号：PCT/KR2013/010765
申请日：2013-11-26
Applicant: 포항공과대학교 산학협력단 , 대한민국(식품의약품안전처장)
IPC: C12Q1/04 , C12Q1/24 , G01N33/569
CPC classification number: G01N33/54333 , C12Q1/04 , C12Q1/24 , G01N33/54326 , G01N33/54346 , G01N33/56911 , G01N33/56916 , G01N33/587 , G01N2333/255
Abstract: 본 발명은 식중독균 검출 방법에 관한 것으로서, 보다 상세하게는 음식물 등에 오염된 식중독균의 함량을 정량적으로 신속하게 분리할 수 있는 방법에 관한 것이다. 본 발명에 따른 방법은 세균을 측정하는 시료에 상기 세균에 결합 가능한 자성 나노입자를 투입하여 세균에 상기 자성 나노입자를 결합시키는 단계; 자성 나노입자를 분리하는 단계; 자력을 이용하여 분리된 자성 나노입자를 고점성 액체에 투과시켜 세균이 결합된 자성 나노입자와 세균이 결합되지 않는 자성 나노입자를 분리하는 단계; 및 세균이 결합된 자성 나노입자를 정량하는 단계를 포함하는 것을 특징으로 한다.
Abstract translation: 本发明涉及一种检测食物中毒细菌的方法，更具体地，涉及一种快速，定量分离污染食物等的食物中毒细菌成分的方法。 根据本发明的方法的特征在于包括以下步骤：将可与细菌结合的磁性纳米颗粒引入用于测量细菌的样品中，以将磁性纳米颗粒结合到细菌上; 分离磁性纳米粒子; 将通过使用磁性分离的纳米颗粒通过具有高粘度的溶液通过，以将与细菌结合的磁性纳米颗粒结合的磁性纳米颗粒分离; 并定量细菌所结合的磁性纳米颗粒。
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公开(公告)号：KR102152386B1
公开(公告)日：2020-09-04
申请号：KR1020130082428
申请日：2013-07-12
Applicant: 고려대학교 산학협력단 , 대한민국 (식품의약품안전처장)
IPC: G01N33/483 , C12Q1/02
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公开(公告)号：KR101523822B1
公开(公告)日：2015-05-28
申请号：KR1020120134544
申请日：2012-11-26
Applicant: 포항공과대학교 산학협력단 , 대한민국 (식품의약품안전처장)
IPC: C12Q1/04 , C12Q1/24 , G01N33/569
CPC classification number: G01N33/54333 , C12Q1/04 , C12Q1/24 , G01N33/54326 , G01N33/54346 , G01N33/56911 , G01N33/56916 , G01N33/587 , G01N2333/255
Abstract: 본발명은식중독균검출방법에관한것으로서, 보다상세하게는음식물등에오염된식중독균의함량을정량적으로신속하게분리할수 있는방법에관한것이다. 상기와같은과제를해결하기위해서, 본발명에따른방법은세균을측정하는시료에상기세균에결합가능한자성나노입자를투입하여세균에상기자성나노입자를결합시키는단계; 자성나노입자를분리하는단계; 자력을이용하여분리된자성나노입자를고점성액체에투과시켜세균이결합된자성나노입자와세균이결합되지않는자성나노입자를분리하는단계; 및세균이결합된자성나노입자를정량하는단계를포함하는것을특징으로한다. 본발명에서와같이, 고점성액체에서자력을이용하여자성나노입자를분리하는방식은세균이결합되지않은자성나노입자가고점성액체에서띠 형태를띠면서세균이결합되는자성나노입자로부터명확하게분리된다는것이다. 원심분리기를이용하는경우는나노입자간의분리능이떨어지는문제가있다. 또한, 본발명에따르는방법은세균과결합할수 있는자성나노입자와자석을제외하고는원심분리기와같은별도의장치를필요로하지않는다는점에서특히유용하다.
Abstract translation: 本发明涉及一种检测食物中毒细菌的方法，更具体地说，涉及一种快速，定量分离污染食物等的食物中毒细菌成分的方法。 根据本发明的方法的特征在于包括以下步骤：将可与细菌结合的磁性纳米颗粒引入用于测量细菌的样品中，以将磁性纳米颗粒结合到细菌上; 分离磁性纳米粒子; 将通过使用磁性分离的纳米颗粒通过具有高粘度的溶液通过，以将与细菌结合的磁性纳米颗粒结合的磁性纳米颗粒分离; 并定量细菌所结合的磁性纳米颗粒。
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4.发明授权식품중 식물성 원료의 진위 여부 판별용 프라이머 세트, 이를 이용한 식품중 식물성 원료의 진위 여부를 판별하는 방법 및 이를 포함하는 키트 有权用于确定食品中植物材料的身份的食品确定方法的入门手册，用于确定使用该食品的食品中的植物材料的身份以及包含该植物材料的试
公开(公告)号：KR101675980B1
公开(公告)日：2016-11-15
申请号：KR1020150063273
申请日：2015-05-06
Applicant: 대한민국 (식품의약품안전처장)
IPC: C12Q1/68
Abstract: 본발명은서열번호 1의정방향프라이머와서열번호 2의역방향프라이머로이루어진프라이머쌍, 서열번호 3의정방향프라이머와서열번호 4의역방향프라이머로이루어진프라이머쌍, 서열번호 5의정방향프라이머와서열번호 6의역방향프라이머로이루어진프라이머쌍, 및서열번호 7의정방향프라이머와서열번호 8의역방향프라이머로이루어진프라이머쌍을포함하는, 식품중식물성원료의진위여부판별용프라이머세트, 이를이용한식품중식물성원료의진위여부를판별하는방법및 이를포함하는키트에관한것이다.
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公开(公告)号：KR101277801B1
公开(公告)日：2013-06-27
申请号：KR1020120059418
申请日：2012-06-01
Applicant: 대한민국 (식품의약품안전처장)
Abstract: PURPOSE: A method for determining the mixture of a root vegetable ingredient using a primer set containing a forward primer and a reverse primer is provided to scientifically monitor foods, and to prevent distribution of bad foods. CONSTITUTION: A method for determining the mixture of a root vegetable ingredient comprises: a step of isolating DNA from a food ingredient; a step of performing first, second, third, and fourth PCR using a primer set; and a step of detecting the presence of the root vegetable ingredient including Panax schinseng, Nopsis lanceolata, Platycodon grandiflorum, and Dioscorea batatas. The primer set is selected from the group consisting of: a primer set comprising a forward primer in sequence number 1 and a reverse primer in sequence number 2 for detecting Panax schinseng; a primer set comprising a forward primer in sequence number 3 and a reverse primer in sequence number 4 for detecting Nopsis lanceolata; a primer set comprising a forward primer in sequence number 5 and a reverse primer in sequence number 6 for detecting Platycodon grandiflorum; and a PCR primer comprising a forward primer in sequence number 7 and a reverse primer in sequence number 8 for detecting Dioscorea batatas.
Abstract translation: 目的：提供一种使用含有正向引物和反向引物的引物组确定根植物成分混合物的方法，以科学监测食物，并防止坏食物的分布。 构成：确定根植物成分的混合物的方法包括：从食品成分中分离DNA的步骤; 使用引物组进行第一，第二，第三和第四PCR的步骤; 以及检测包括人参，Nopsis lanceolata，Platycodon grandiflorum和薯a薯等根系植物成分的存在的步骤。 引物组选自：包含序列号1的正向引物的引物组和序列号2的用于检测人参的反向引物; 包含序列号3的正向引物和序列号4的反向引物的引物组，用于检测花椒（Nopsis lanceolata）; 一种引物组，其包含序列号5的正向引物和序列号6中用于检测桔梗的反向引物; 和包含序列号7的正向引物和序列号8的反向引物的PCR引物，用于检测薯detecting皂甙。
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Patent Agency Ranking
公开(公告)号：KR101277800B1
公开(公告)日：2013-06-27
申请号：KR1020120059417
申请日：2012-06-01
Applicant: 대한민국 (식품의약품안전처장)
Abstract: PURPOSE: A technique for determining the mixture of a mollusks ingredient in a food using a primer set containing a forward primer of a certain sequence and a reverse primer of a certain sequence is provided to enable scientific monitoring of a food, and to prevent distribution of bad food. CONSTITUTION: A method for determining the mixture of a mollusks ingredient in a food comprises: a step of isolating DNA from the food; a step of performing first and second PCR using a primer set and DNA as a template; and a step of detecting the presence of the mollusks ingredient in the food. The primer set is selected from the group consisting of: a primer set containing a forward primer in sequence number 1 and a reverse primer in sequence number 2 for detecting Todarodes pacificus; and a primer set containing a forward primer in sequence number 3 and a reverse primer in sequence number 4 for detecting Loligo bleekeri.
Abstract translation: 目的：提供一种用于使用含有某一序列的正向引物和一定序列的反向引物的引物组确定食品中软体动物成分的混合物的技术，以使科学监测食物，并防止 不好的食物。 构成：确定食品中软体动物成分的混合物的方法包括：从食物中分离DNA的步骤; 使用引物组和DNA作为模板进行第一和第二PCR的步骤; 以及检测食品中软体动物成分的存在的步骤。 引物组选自：包含序列号1的正向引物的引物组和序列号为2的反向引物，用于检测太平洋假丝酵母; 和含有序列号3的正向引物和序列号4的反向引物的引物组，用于检测Loligo bleekeri。
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公开(公告)号：KR1020150114037A
公开(公告)日：2015-10-12
申请号：KR1020140037537
申请日：2014-03-31
Applicant: 포항공과대학교 산학협력단 , 대한민국 (식품의약품안전처장)
CPC classification number: Y02A50/451
Abstract: 본발명은병원성단백질및 미생물의검출방법에관한것으로서, 보다상세하게는피펫과고점성용액및 자성나노입자를이용하여음식물등에포함된미생물이나병원성단백질을신속하게검출하고정량화시킬수 있는방법에관한것이다.상기와같은과제를해결하기위해서, 본발명에따른방법은병원성물질을포함하는시료에병원성물질에결합가능한자성나노입자를투입하여결합시키는단계; 자성나노입자를분리하는단계; 분리된자성나노입자를자력을이용하여고점성액체에투과시켜병원성물질이결합된자성나노입자를분리하는단계; 및분리된병원성물질이결합된자성나노입자를분석하는단계를포함하는병원성물질의측정방법을제공한다. 본발명에서는고점성유체에서분리상으로존재하는자성나노입자가결합된병원성물질을피펫을이용해서선택적으로분리할수 있는방법이제시되었으며, 이로인해자성나노입자가결합된병원성물질을고점성유체로부터분리하는과정에서발생하는오차를줄일수 있게된다. 또한, 본발명에서는고점성유체로부터병원성물질이결합된자성나노입자를이용하여신호증폭을통해서정량분석의정확도를향상시킬수 있는방법이제시되었다.
Abstract translation: 本发明涉及一种检测致病性蛋白质和微生物的方法。 更具体地，本发明涉及通过使用移液管，高粘度溶液和磁性纳米颗粒来快速检测和定量食品等中包含的微生物或病原性蛋白质的方法。 为了解决这个问题，本发明的方法包括以下步骤：将包含致病物质的样品中的能与病原物质偶联的磁性纳米颗粒进料和偶联; 分离磁性纳米颗粒; 通过使分离的磁性纳米颗粒通过使用磁性渗透高粘度液体来分离与病原物质结合的磁性纳米颗粒; 并分析与致病物质结合的分离的磁性纳米颗粒。 根据本发明，公开了一种通过使用移液管从高粘性流体中选择性分离与单独存在的磁性纳米颗粒结合的致病物质的方法。 因此，可以减少在与高粘性流体混合磁性纳米粒子的病原物质分离步骤中发生的错误。 此外，在本发明中，公开了通过使用与来自高粘性流体的致病物质结合的磁性纳米颗粒通过信号放大来提高定量分析的准确度的方法。
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公开(公告)号：KR101445596B1
公开(公告)日：2014-10-07
申请号：KR1020120108074
申请日：2012-09-27
Applicant: 단국대학교 산학협력단 , 대한민국 (식품의약품안전처장)
CPC classification number: G01N33/12 , G01N33/533 , G01N33/54326 , G01N33/552 , G01N33/582
Abstract: 항생제 농도의 측정 방법 및 측정 키트가 제공된다. 본 발명의 일 실시예에 따른 항생제 농도의 측정 방법은, 항생제와 결합된 자성 입자를 준비하는 단계, 항생제의 항체가 하나 이상 결합된 실리카 코팅 형광 입자를 준비하는 단계, 자성 입자를 실리카 코팅 형광 입자와 반응시키는 단계, 및 반응된 실리카 코팅 형광 입자에 레이저를 조사하는 단계를 포함한다.
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公开(公告)号：KR1020140042065A
公开(公告)日：2014-04-07
申请号：KR1020120108074
申请日：2012-09-27
Applicant: 단국대학교 산학협력단 , 대한민국 (식품의약품안전처장)
CPC classification number: G01N33/12 , G01N33/533 , G01N33/54326 , G01N33/552 , G01N33/582
Abstract: Provided are a method and a kit for measuring the concentration of antibiotics. The method for measuring the concentration of antibiotics according to one embodiment of the present invention includes a step of preparing magnetic particles combined with antibiotics, a step of preparing silica coated fluorescent particles combined with one or more antibodies of antibiotics, a step of making the magnetic particles react with the fluorescent particles, and a step of making the silica coated fluorescent particles be irradiated with lasers. [Reference numerals] (AA) Start; (BB) End; (S11) Step of pre-processing antibiotics; (S12) Step of coupling an amine group on the surface of magnetic particles; (S13) Step of adding a cross linking agent to the magnetic particles in which the amine group is combined; (S14) Step of combining the magnetic particles in which the amine group is combined with antibiotics; (S15) Step of collecting the magnetic particles combined with the antibiotics by using magnetic force; (S21) Step of forming coated fluorescent particles by coating the fluorescent particles with silica; (S22) Step of combining a carboxyl group on the surface of silica coated fluorescent particles; (S23) Step of adding the cross linking agent to the silica coated fluorescent particles combined with the carboxyl group; (S24) Step of combining antibodies of the antibiotics and the silica coated fluorescent particles combined with the carboxyl group; (S25) Step of measuring the antibodies amount of the antibiotics combined with the silica coated fluorescent particles; (S3) Step of reacting the magnetic particles with the silica coated fluorescent particles; (S4) Step of collecting the magnetic particles reacting to the silica coated fluorescent particles by using a magnet; (S5) Step of measuring the concentration of the antibiotics by irradiating the silica coated fluorescent particles with a laser
Abstract translation: 提供了用于测量抗生素浓度的方法和试剂盒。 根据本发明的一个实施方案的用于测量抗生素浓度的方法包括制备与抗生素组合的磁性颗粒的步骤，制备与一种或多种抗生素抗体结合的二氧化硅涂覆的荧光颗粒的步骤，制备磁性的步骤 颗粒与荧光颗粒反应，并且用激光照射二氧化硅涂覆的荧光颗粒的步骤。 （附图标记）（AA）开始; （BB）结束; （S11）预处理抗生素的步骤; （S12）将磁性粒子表面上的胺基团偶联的工序; （S13）将交联剂加入其中胺基团的磁性颗粒中的步骤; （S14）将胺基与抗生素组合的磁性粒子组合的工序; （S15）使用磁力收集与抗生素结合的磁性粒子的步骤; （S21）通过用二氧化硅涂布荧光体颗粒来形成涂布的荧光颗粒的步骤; （S22）在二氧化硅被覆荧光体颗粒的表面上组合羧基的工序; （S23）将交联剂添加到与羧基组合的二氧化硅被覆荧光粒子上的工序; （S24）将抗生素的抗体与与羧基结合的二氧化硅被覆荧光粒子结合的步骤; （S25）测定与二氧化硅被覆荧光粒子结合的抗生素的抗体量的步骤; （S3）磁性颗粒与二氧化硅涂覆的荧光颗粒反应的步骤; （S4）使用磁铁收集与二氧化硅涂布的荧光体粒子反应的磁性粒子的工序; （S5）通过用激光照射二氧化硅被覆荧光粒子来测定抗生素浓度的步骤
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公开(公告)号：KR1020130078657A
公开(公告)日：2013-07-10
申请号：KR1020110147723
申请日：2011-12-30
Applicant: 켐아이넷(주) , 대한민국 (식품의약품안전처장)
CPC classification number: Y02A90/22 , G06F17/30572 , G06Q50/22
Abstract: PURPOSE: A data mining method for food safety responding to climate change is provided to search data required for safe food management by reflecting climate change factors, thereby accurately managing food in responding to climate change. CONSTITUTION: A region for data mining, the climate factor of the region, and the temporal range of the climate factor are selected (S100). A hazard factor to be used for data mining is selected among hazard factors caused by harmful materials (S110). A search is performed in a database, which has monitoring data and the climate factor data, in connection with the region, climate factor, temporal range, and hazard factor (S120). A linked search calculates the amount of a change in the climate factor in the temporal range and searches the database based on the change. [Reference numerals] (S100) Region for data mining, the climate factor of the region, and the temporal range of the climate factor are selected; (S110) Hazard factor to be used for data mining is selected among hazard factors caused by harmful materials; (S120) Search is performed in a database, which has monitoring data and the climate factor data, in connection with the region, climate factor, temporal range, and hazard factor
Abstract translation: 目的：提供应对气候变化的食品安全数据挖掘方法，通过反映气候变化因素来搜索安全食品管理所需的数据，从而准确地管理食物应对气候变化。 规定：选择数据挖掘区域，该区域的气候因子和气候因素的时间范围（S100）。 选择用于数据挖掘的危险因素是由有害物质引起的危险因素（S110）。 在数据库中执行搜索，该数据库具有与区域，气候因素，时间范围和危险因素有关的监测数据和气候因素数据（S120）。 链接搜索计算时间范围内气候因子变化的量，并根据变化搜索数据库。 （附图标记）（S100）选择用于数据挖掘的区域，区域的气候因子和气候因子的时间范围; （S110）用于数据挖掘的危害因素是由有害物质引起的危险因素中选择的; （S120）在具有监测数据和气候因子数据的数据库中执行搜索，与区域，气候因素，时间范围和危害因素有关
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