식품원료 내 식육원료의 혼입여부를 판별하는 방법
    1.
    发明授权
    식품원료 내 식육원료의 혼입여부를 판별하는 방법 有权
    用于物种鉴定的PCR引物的开发

    公开(公告)号:KR101277798B1

    公开(公告)日:2013-07-09

    申请号:KR1020120059414

    申请日:2012-06-01

    CPC classification number: C12Q1/686 G01N33/02

    Abstract: PURPOSE: A method for identifying the mixture of meat ingredients in a food ingredient is provided to enable scientific food monitoring and to block distribution of bad foods. CONSTITUTION: A method for identifying the mixture of meat ingredients in a food ingredient comprises: a step of extracting DNA from the food ingredient; a step of performing PCR comprising first, second, third, fourth, fifth, and sixth steps using the DNA and a primer set; and a step of detecting the presence of meats including bos taurus, sus scrofa, ovis aries, capra hircus, cervus elaphus, cervus Nippon, and equus caballus.

    Abstract translation: 目的:提供一种鉴定食品中肉类成分混合物的方法,以便科学的食品监测和阻止不良食品的分销。 构成:用于鉴定食品成分中的肉成分混合物的方法包括:从食品成分中提取DNA的步骤; 使用DNA和引物组进行包含第一,第二,第三,第四,第五和第六步骤的PCR的步骤; 检测肉骨的存在的步骤,包括金牛座,苏格兰黑猩猩,奥们白俄罗斯,披肩,鹿耳鹿,日本母鹿和马鞍马。

    식품원료 내 근채류 원료의 혼입여부를 판별하는 방법
    3.
    发明授权
    식품원료 내 근채류 원료의 혼입여부를 판별하는 방법 有权
    开发用于物种鉴定的根系的PCR引物

    公开(公告)号:KR101277801B1

    公开(公告)日:2013-06-27

    申请号:KR1020120059418

    申请日:2012-06-01

    CPC classification number: C12Q1/686 G01N33/02

    Abstract: PURPOSE: A method for determining the mixture of a root vegetable ingredient using a primer set containing a forward primer and a reverse primer is provided to scientifically monitor foods, and to prevent distribution of bad foods. CONSTITUTION: A method for determining the mixture of a root vegetable ingredient comprises: a step of isolating DNA from a food ingredient; a step of performing first, second, third, and fourth PCR using a primer set; and a step of detecting the presence of the root vegetable ingredient including Panax schinseng, Nopsis lanceolata, Platycodon grandiflorum, and Dioscorea batatas. The primer set is selected from the group consisting of: a primer set comprising a forward primer in sequence number 1 and a reverse primer in sequence number 2 for detecting Panax schinseng; a primer set comprising a forward primer in sequence number 3 and a reverse primer in sequence number 4 for detecting Nopsis lanceolata; a primer set comprising a forward primer in sequence number 5 and a reverse primer in sequence number 6 for detecting Platycodon grandiflorum; and a PCR primer comprising a forward primer in sequence number 7 and a reverse primer in sequence number 8 for detecting Dioscorea batatas.

    Abstract translation: 目的:提供一种使用含有正向引物和反向引物的引物组确定根植物成分混合物的方法,以科学监测食物,并防止坏食物的分布。 构成:确定根植物成分的混合物的方法包括:从食品成分中分离DNA的步骤; 使用引物组进行第一,第二,第三和第四PCR的步骤; 以及检测包括人参,Nopsis lanceolata,Platycodon grandiflorum和薯a薯等根系植物成分的存在的步骤。 引物组选自:包含序列号1的正向引物的引物组和序列号2的用于检测人参的反向引物; 包含序列号3的正向引物和序列号4的反向引物的引物组,用于检测花椒(Nopsis lanceolata); 一种引物组,其包含序列号5的正向引物和序列号6中用于检测桔梗的反向引物; 和包含序列号7的正向引物和序列号8的反向引物的PCR引物,用于检测薯detecting皂甙。

    식품원료 내 연체류 원료의 혼입여부를 판별하는 방법
    4.
    发明授权
    식품원료 내 연체류 원료의 혼입여부를 판별하는 방법 有权
    用于物种鉴定的PCR引物的开发

    公开(公告)号:KR101277800B1

    公开(公告)日:2013-06-27

    申请号:KR1020120059417

    申请日:2012-06-01

    CPC classification number: C12Q1/686 G01N33/02

    Abstract: PURPOSE: A technique for determining the mixture of a mollusks ingredient in a food using a primer set containing a forward primer of a certain sequence and a reverse primer of a certain sequence is provided to enable scientific monitoring of a food, and to prevent distribution of bad food. CONSTITUTION: A method for determining the mixture of a mollusks ingredient in a food comprises: a step of isolating DNA from the food; a step of performing first and second PCR using a primer set and DNA as a template; and a step of detecting the presence of the mollusks ingredient in the food. The primer set is selected from the group consisting of: a primer set containing a forward primer in sequence number 1 and a reverse primer in sequence number 2 for detecting Todarodes pacificus; and a primer set containing a forward primer in sequence number 3 and a reverse primer in sequence number 4 for detecting Loligo bleekeri.

    Abstract translation: 目的:提供一种用于使用含有某一序列的正向引物和一定序列的反向引物的引物组确定食品中软体动物成分的混合物的技术,以使科学监测食物,并防止 不好的食物。 构成:确定食品中软体动物成分的混合物的方法包括:从食物中分离DNA的步骤; 使用引物组和DNA作为模板进行第一和第二PCR的步骤; 以及检测食品中软体动物成分的存在的步骤。 引物组选自:包含序列号1的正向引物的引物组和序列号为2的反向引物,用于检测太平洋假丝酵母; 和含有序列号3的正向引物和序列号4的反向引物的引物组,用于检测Loligo bleekeri。

    식품원료 내 어류 원료의 혼입여부를 판별하는 방법
    5.
    发明授权
    식품원료 내 어류 원료의 혼입여부를 판별하는 방법 有权
    用于鉴定鱼类的PCR引物的开发

    公开(公告)号:KR101219763B1

    公开(公告)日:2013-01-24

    申请号:KR1020120059416

    申请日:2012-06-01

    CPC classification number: C12Q1/686 G01N33/02

    Abstract: PURPOSE: A method for determining the mixture of a fish ingredient in a food ingredient using a PCR prime set is provided to scientifically monitor foods, and to distribute and manage safety foods. CONSTITUTION: A method for determining the mixture of Oreochromis niloticus in a food ingredient comprises a step of using a PCR primer set containing a forward primer of sequence number 7 and a reverse primer of sequence number 8. The PCR primer set for determining the mixture contains the forward and reverse primers.

    Abstract translation: 目的:提供一种使用PCR引物确定食品成分中鱼成分混合物的方法,以科学监测食物,并分发和管理安全食品。 构成:用于测定食品成分中的Oreochromis niloticus的混合物的方法包括使用含有序列号7的正向引物和序列号8的反向引物的PCR引物组的步骤。用于测定混合物的PCR引物组含有 正向和反向引物。

    식중독균 검출 키트 및 이를 이용한 식중독균 검출 방법
    7.
    发明公开
    식중독균 검출 키트 및 이를 이용한 식중독균 검출 방법 有权
    用于检测食物杆菌的试剂盒和方法

    公开(公告)号:KR1020140011086A

    公开(公告)日:2014-01-28

    申请号:KR1020120077731

    申请日:2012-07-17

    Abstract: The present invention relates to a food poisoning bacteria detection kit and a method for detecting the three kinds of food poisoning bacteria by using the same. In accordance with an embodiment of the present invention, the food poisoning bacteria detection kit can effectively detect the three kinds of the food poisoning bacteria at the same time. The food poisoning bacteria detection kit includes RNA aptamer including base sequence of sequence number 1 specifically combined with ompC protein of Salmonella; RNA aptamer including the base sequence of sequence number 2 specifically combined with teichoic acid of Staphylococcus; and RNA aptamer including the base sequence of sequence number 3 specifically combined with lipopolysaccharide (LPS) of Escherichia coli O157:H7.

    Abstract translation: 本发明涉及一种食物中毒细菌检测试剂盒和使用该食物中毒细菌的三种食物中毒细菌的检测方法。 根据本发明的实施例,食物中毒细菌检测试剂盒可以同时有效地检测三种食物中毒细菌。 食物中毒细菌检测试剂盒包括RNA适体,其包括序列号1的碱基序列特异性结合沙门氏菌的ompC蛋白; RNA适体包括序列号2的碱基序列与葡萄球菌的磷壁酸特异性结合; 和包含特异性结合大肠杆菌O157:H7的脂多糖(LPS)的序列号3的碱基序列的RNA适体。

    식품원료 내 가금류 원료의 혼입여부를 판별하는 방법
    8.
    发明授权
    식품원료 내 가금류 원료의 혼입여부를 판별하는 방법 有权
    用于物种鉴定的PCR引物的开发

    公开(公告)号:KR101277799B1

    公开(公告)日:2013-06-27

    申请号:KR1020120059415

    申请日:2012-06-01

    CPC classification number: C12Q1/686 G01N33/02

    Abstract: PURPOSE: A method for determining the mixture of a poultry ingredient in a food using a primer set comprising a forward primer of a certain sequence and a reverse primer of a certain sequence is provided to scientifically monitor foods for prevent distribution of bad foods. CONSTITUTION: A method for determining the mixture of a poultry ingredient in a food comprises: a step of isolating DNA; a step of performing first, second, third, and fourth PCR using a primer set and the DNA as a template; and a step of detecting the poultry ingredient. The primer set is selected from the group consisting of: a primer set comprising a forward primer in sequence number 1 and a reverse primer in sequence number 2 for detecting Gallus gallus; a primer set comprising a forward primer in sequence number 3 and a reverse primer in sequence number 4 for detecting Anas platyrhynchos; a primer set comprising a forward primer in sequence number 5 and a reverse primer in sequence number 6 for detecting Meleagris gallopago; and a primer comprising a forward primer in sequence number 7 and a reverse primer in sequence number 8 for detecting Struthio camelus.

    Abstract translation: 目的:提供一种使用包含某一序列的正向引物和一定序列的反向引物的引物组确定食品中家禽成分的混合物的方法,以科学监测食物以防止坏食物的分布。 构成:确定食品中家禽成分混合物的方法包括:分离DNA的步骤; 使用引物组和DNA作为模板进行第一,第二,第三和第四PCR的步骤; 以及检测家禽成分的步骤。 引物组选自:引物组,其包含序列号1的正向引物和序列号为2的反向引物,用于检测Gallus gallus; 包含序列号3的正向引物和序列号4的反向引物的引物组,用于检测Anas platyrhynchos; 序列号5的正向引物和序列号6的反向引物的引物组,用于检测Meleagris gallopago; 和包含序列号7的正向引物和序列号8的反向引物的引物,用于检测骆驼属。

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