公开(公告)号：KR1020040110730A

公开(公告)日：2004-12-31

申请号：KR1020030040171

申请日：2003-06-20

Applicant: 대한민국 (식품의약품안전처장)

Inventor： 조정식 ,  김용규 ,  강태석 ,  황진희 ,  황대연 ,  임채형 ,  백상기

IPC: A01K67/027

CPC classification number: A01K67/0276 ,  A01K2227/105 ,  C12N15/63

Abstract: PURPOSE: A transgenic Alzheimer's mice expressing APPsw gene and a preparation method thereof are provided to show almost perfect Alzheimer symptoms and to be used in developing an Alzheimer treatment and an early diagnosis manner. CONSTITUTION: The preparation method of the transgenic Alzheimer's mice expressing APPsw(Amyloid precursor protein swedish) gene comprises the steps of: preparing a recombinant fusion gene by connecting an APPsw gene to a promoter of a NSE(Neur on-Specific Enolase) gene; microinjecting the recombinant fusion gene into a fertilized egg of a mouse; and breeding by nidating the fertilized egg into an abduct of a surrogate mother mouse.

Abstract translation: 目的：提供表达APPsw基因的转基因阿尔茨海默氏小鼠及其制备方法，以显示几乎完美的阿尔茨海默症状，并用于开发阿尔茨海默病和早期诊断。 构成：表达APPsw（淀粉样前体蛋白质瑞典）基因的转基因阿尔茨海默氏小鼠的制备方法包括以下步骤：通过将APPsw基因连接到NSE（Neur on-Specific Enolase）基因的启动子来制备重组融合基因; 将重组融合基因显微注射入小鼠的受精卵中; 并通过将受精卵覆盖到替代母亲小鼠的绑架中进行繁殖。

公开(公告)号：KR100441202B1

公开(公告)日：2004-07-23

申请号：KR1020010078140

申请日：2001-12-11

Applicant: 대한민국 (식품의약품안전처장)

Inventor： 조정식 ,  채갑용 ,  김용규 ,  임철주 ,  신동환 ,  장인석 ,  황진희 ,  황대연 ,  조용연 ,  김연주 ,  백상기

IPC: A01K67/00

Abstract: PURPOSE: Provided is a transformed mouse expressing the HPV E6 gene which is usefully used in screening tumor gene and tumor treating agent since tumor development occurs at the late stage. CONSTITUTION: An animal excluding human beings is transformed to express HPV(human papilloma virus) E6 gene inducing the mammary gland tumor. In the transformed animal, the HPV E6 gene is inserted into the chromosome of the animal and is operably linked to a beta-casein promotor. The chromosome is able to be body cells or sexual cells. The animal is mainly a mouse.

Abstract translation: 目的：提供一种表达HPV E6基因的转化的小鼠，由于在晚期发生肿瘤基因和肿瘤治疗剂，所以它被有效地用于筛选肿瘤基因和肿瘤治疗剂。 构成：将除人以外的动物转化为表达诱导乳腺肿瘤的HPV（人乳头状瘤病毒）E6基因。 在转化的动物中，将HPV E6基因插入动物的染色体并可操作地连接至β-酪蛋白启动子。 染色体可以是身体细胞或有性细胞。 该动物主要是一只老鼠。

公开(公告)号：KR1020030048253A

公开(公告)日：2003-06-19

申请号：KR1020010078140

申请日：2001-12-11

Applicant: 대한민국 (식품의약품안전처장)

Inventor： 조정식 ,  채갑용 ,  김용규 ,  임철주 ,  신동환 ,  장인석 ,  황진희 ,  황대연 ,  조용연 ,  김연주 ,  백상기

IPC: A01K67/00

CPC classification number: A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/0318 ,  C12N15/8509

Abstract: PURPOSE: Provided is a transformed mouse expressing the HPV E6 gene which is usefully used in screening tumor gene and tumor treating agent since tumor development occurs at the late stage. CONSTITUTION: An animal excluding human beings is transformed to express HPV(human papilloma virus) E6 gene inducing the mammary gland tumor. In the transformed animal, the HPV E6 gene is inserted into the chromosome of the animal and is operably linked to a beta-casein promotor. The chromosome is able to be body cells or sexual cells. The animal is mainly a mouse.

Abstract translation: 目的：提供表达HPV E6基因的转化小鼠，其可用于筛选肿瘤基因和肿瘤治疗剂，因为肿瘤发生在晚期发生。 构成：不包括人类的动物被转化以表达诱导乳腺肿瘤的HPV（人乳头瘤病毒）E6基因。 在转化的动物中，将HPV E6基因插入到动物的染色体中，并可操作地连接到β-酪蛋白启动子。 染色体能够是身体细胞或性细胞。 动物主要是一只老鼠。

公开(公告)号：KR1020040076735A

公开(公告)日：2004-09-03

申请号：KR1020030012079

申请日：2003-02-26

Applicant: 대한민국 (식품의약품안전처장)

Inventor： 조정식 ,  김용규 ,  강태석 ,  황진희 ,  황대연 ,  임채형 ,  백상기

IPC: A01K67/027

CPC classification number: A01K67/0276 ,  A01K2227/105 ,  C12N15/09

Abstract: PURPOSE: A transgenic dementia mouse expressing a mutant PS2 gene and a manufacturing process thereof are provided to be utilized usefully in treating a dementia disease or improving an early diagnosis process of the disease. CONSTITUTION: The manufacturing process of the transgenic dementia mouse expressing a mutant PS2 gene comprises the steps of: preparing a hybrid gene NSE/hPS2m by connecting to a promoter of a neuron-specific enolase gene to a mutant PS2 dementia gene derived from a human; micro-injecting the hybrid gene into a fertilized egg of a mouse; and implanting the fertilized egg into an oviduct of a surrogate mother mouse.

Abstract translation: 目的：提供一种表达突变型PS2基因的转基因痴呆小鼠及其制备方法，可用于治疗痴呆疾病或改善疾病的早期诊断过程。 构成：表达突变型PS2基因的转基因痴呆小鼠的制造方法包括以下步骤：通过将神经元特异性烯醇化酶基因的启动子连接到源自人的突变型PS2痴呆基因来制备杂合基因NSE / hPS2m; 将杂交基因微注射到小鼠的受精卵中; 并将受精卵植入代孕母鼠的输卵管中。