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公开(公告)号：WO2019117427A1
公开(公告)日：2019-06-20
申请号：PCT/KR2018/009757
申请日：2018-08-23
Applicant: 연세대학교 산학협력단 , 동아대학교 산학협력단
Abstract: 본 발명은 서열번호 1의 16S rDNA를 포함하고, 풋마름병을 비롯한 식물병에 대한 방제 효과 또는 식물 생장 촉진 효과를 가지는 플라보박테리아( Flavobacteriaceae ) 과 균주가 제공된다.
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公开(公告)号：KR101347111B1
公开(公告)日：2014-01-03
申请号：KR1020120110485
申请日：2012-10-05
Applicant: 동아대학교 산학협력단
Abstract: The present invention relates to an esterase ELP86 gene derived from soil metagenome and a use thereof, which can be effectively used for various industries by confirming the decomposition of extracellular polysaccharide on plant pathogenic fungi using protein induced from esterase ELP86 genes separated from the present invention and developing a microorganism product for controlling plant pathogenic fungi or plant pathogenic fungus resistant crops using microorganisms secreting the protein.
Abstract translation: 本发明涉及来自土壤微生物群落的酯酶ELP86基因及其用途，其可以通过使用从本发明分离的酯酶ELP86基因诱导的蛋白质确认细胞外多糖对植物致病真菌的分解而有效地用于各种工业，以及 利用分泌蛋白质的微生物开发用于控制植物致病真菌或植物病原真菌抗性作物的微生物产物。
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公开(公告)号：KR1020130095518A
公开(公告)日：2013-08-28
申请号：KR1020120017020
申请日：2012-02-20
Applicant: 동아대학교 산학협력단
IPC: C07K14/195 , C12N15/31 , C12N15/82 , A01H5/00
CPC classification number: C12N9/1029 , C12N15/8271 , C12N15/8273
Abstract: PURPOSE: A transformant containing overexpressed soil metagenome-derived wax ester synthase (WES) gene derived from soil metagenome is provided to produce a large amount of wax ester and to create economic benefit in food and pharmaceutics industries as well as a cosmetics industry. CONSTITUTION: A soil metagenome-derived WES protein related to wax ester production or environmental stress resistance contains an amino acid sequence of sequence number 2. A gene encodes the protein. The gene has a base sequence of sequence number 1. A recombinant vector contains the gene. A method for enhancing the production of wax ester comprises the steps of transforming plant cells with the recombinant vector and overexpressing WES gene. [Reference numerals] (AA) MS+mannitol 200 mM; (BB) MS medium (control)
Abstract translation: 目的：提供一种含有源自土壤元素的过表达土壤巨蛋白衍生的蜡酯合成酶（WES）基因的转化体，以生产大量的蜡酯，并为食品和药物行业以及化妆品行业创造经济效益。 构成：与蜡酯生产或环境胁迫相关的土壤巨型突变体衍生的WES蛋白质含有序列号2的氨基酸序列。基因编码蛋白质。 该基因具有序列号1的碱基序列。重组载体含有该基因。 提高蜡酯生产的方法包括用重组载体转化植物细胞并过量表达WES基因的步骤。 （AA）MS +甘露糖醇200mM; （BB）MS培养基（对照）
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公开(公告)号：KR1020120055801A
公开(公告)日：2012-06-01
申请号：KR1020100117196
申请日：2010-11-24
Applicant: 동아대학교 산학협력단
Abstract: PURPOSE: A bacteriophage isolated from soil is provided to ensure specific lysis activity toward Ralstonia solanacearum and to develop an agent for preventing bacterial wilt in various plants. CONSTITUTION: Bacteriophage PE226(deposit number: KACC 97007P) has a lysis activity specific to Ralstonia solanacearum. The bacteriophage has a genome having a base of sequence number 1. The bacteriophage is active at 25°C-60°C and a pH of 4-11. A composition for preventing plant diseases caused by the Ralstonia solanacearum contains the bacteriophage as an active ingredient. The plant diseases include plant bacterial wilt. A method for preventing plant diseases comprises a step of applying the composition onto plants.
Abstract translation: 目的：提供从土壤中分离出的噬菌体，以确保针对罗勒氏菌（Ralstonia solanacearum）的特异性裂解活性，并开发用于预防各种植物中的细菌性枯萎病的药剂。 构成：噬菌体PE226（保藏号：KACC 97007P）具有针对Ralstonia solanacearum的裂解活性。 噬菌体具有序列号1的碱基的基因组。噬菌体在25℃-60℃和4-11的pH下是有活性的。 用于防止由雷氏弧菌引起的植物病害的组合物含有噬菌体作为活性成分。 植物病害包括植物细菌枯萎病。 防止植物病害的方法包括将组合物施用于植物的步骤。
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公开(公告)号：KR101077913B1
公开(公告)日：2011-10-31
申请号：KR1020090011087
申请日：2009-02-11
Applicant: 한국화학연구원 , 동아대학교 산학협력단
IPC: C12N9/16 , C07K14/195 , C12N15/09 , A01H1/00
Abstract: 본발명은토양메타게놈유래신규에스터라제(esterase) 유전자및 이로부터암호화되는에스터라제단백질에관한것으로, 보다상세하게는토양미생물의총 유전체인메타게놈으로부터분리한신규에스터라제가우수한에스터라제활성을나타내고, 온도, pH 및유기용매에대한효소활성안정성을나타내며, 짧은사슬(short chain) 지방산을가지는지질및 3-하이드록시팔미트산메틸에스테르(3-OH palmitic acid methyl ester; 3-OH PAME)를분해하여식물병원성균에대한바이오필름저해활성을나타냄으로써식물세균병저항성작물또는식물세균병제어제개발에유용하게사용될수 있다.
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Patent Agency Ranking
公开(公告)号：KR1020100098964A
公开(公告)日：2010-09-10
申请号：KR1020090017701
申请日：2009-03-02
Applicant: 동아대학교 산학협력단
Abstract: PURPOSE: A novel ubiquitin-conjugated enzyme E2 is provided to have a pivotal role in a plant stress resistance reaction and to be used in molecular genetic research. CONSTITUTION: A ubiquitin-conjugated enzyme E2 comprises an amino acid sequence of sequence number 2. The ubiquitin-conjugated enzyme E2 is isolated from mung beans. The expression of ubiquitin-conjugated enzyme E2 is induced by stress. The stress is ABA(abscisic acid) or bittern. A gene encoding the ubiquitin-conjugated enzyme E2 comprises a base sequence of sequence number 1. A recombinant plasmid pBAD-MLT13 contains the gene encoding the ubiquitin-conjugated enzyme.
Abstract translation: 目的：提供一种新的泛素结合酶E2，以在植物胁迫反应中具有关键作用，并用于分子遗传研究。 构成：泛素结合酶E2包含序列号2的氨基酸序列。泛绿蛋白结合酶E2从绿豆中分离。 泛素结合酶E2的表达是由应激引起的。 应激是ABA（脱落酸）或卤水。 编码泛素结合酶E2的基因包含序列号1的碱基序列。重组质粒pBAD-MLT13含有编码泛素缀合的酶的基因。
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公开(公告)号：KR1020100091749A
公开(公告)日：2010-08-19
申请号：KR1020090011087
申请日：2009-02-11
Applicant: 한국화학연구원 , 동아대학교 산학협력단
IPC: C12N9/16 , C07K14/195 , C12N15/09 , A01H1/00
Abstract: PURPOSE: A soil metagenome-derived novel esterase gene is provided to ensure enzyme activity stability and to use in developing an agent which controls plant bacterial diseases. CONSTITUTION: A polypeptide having esterase activity comprises: a polypeptide encoded by polynucleotide of sequence number 1; a polypeptide encoded by a polynucleotide which is hybridized with the polynucleotide of sequence number 1; and a polypeptide with an amino acid sequence of sequence number 2. A method for preparing a recombinant esterase comprises: a step of preparing a recombinant expression vector containing the polynueotide; a step of preparing a transformant by introducing a recombinant expression vector to host cells; and a step of culturing the transformant to induce recombinant protein expression.
Abstract translation: 目的：提供一种土壤巨蛋白衍生新型酯酶基因，以确保酶活性的稳定性，并用于开发控制植物细菌性疾病的药剂。 构成：具有酯酶活性的多肽包括：由序列号1的多核苷酸编码的多肽; 由与序列号1的多核苷酸杂交的多核苷酸编码的多肽; 和具有序列号2的氨基酸序列的多肽。制备重组酯酶的方法包括：制备含有多核苷酸的重组表达载体的步骤; 通过将重组表达载体引入宿主细胞来制备转化体的步骤; 以及培养转化体诱导重组蛋白表达的步骤。
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公开(公告)号：KR1020100022371A
公开(公告)日：2010-03-02
申请号：KR1020080081013
申请日：2008-08-19
Applicant: 동아대학교 산학협력단
Abstract: PURPOSE: An RNA helicase gene and environment stress tolerance plant cell or plant body are provided to overexpressed in a plant cell by environmental stress and ensure tolerance to the environmental stress. CONSTITUTION: An RNA helicase is denoted by an amino acid sequence of sequence number 2. A gene encoding the RNA helicase is denoted by sequence number 1. The RNA helicase gene is isolated thorugh RACE PCR(Rapid amplification of cDNA ends polymerase chain reaction) method based on cDNA gene library of a fragment of which expression is induced by low temperature in a soybean. A recombinant expression vector contains RNA helicase gene of sequence number 1. A transformed microorganism is prepared by the recombinant expression vector. The environmental stress is low temperature, moisture, bittern or abscisic acid(ABA).
Abstract translation: 目的：提供RNA解旋酶基因和环境胁迫耐受植物细胞或植物体，通过环境胁迫在植物细胞中过表达，并确保对环境胁迫的耐受性。 构成：RNA解旋酶由序列号2的氨基酸序列表示。编码RNA解旋酶的基因由序列号1表示。RNA解旋酶基因是分离的RACE PCR（cDNA末端聚合酶链式反应的快速扩增）方法 基于大豆中由低温诱导表达的片段的cDNA基因文库。 重组表达载体含有序列号1的RNA解旋酶基因。通过重组表达载体制备转化的微生物。 环境胁迫是低温，潮湿，盐渍或脱落酸（ABA）。
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9.发明公开녹두로부터 분리한 2-CYS PEROXIREDOXIN 유전자(Vrmlt63)의 염기서열, 아미노산 서열 및 유전자 기능 无效神经元序列，氨基酸序列和2-CYS过氧化物酶基因（VRMLT63）在昆明的功能
公开(公告)号：KR1020090033749A
公开(公告)日：2009-04-06
申请号：KR1020070098932
申请日：2007-10-01
Applicant: 동아대학교 산학협력단
Abstract: Provided is an apoptosis-related 2-cysteine peroxiredoxin gene(Vrmlt63), which is separated from mung beans and expressed by stresses. A 2-cysteine peroxiredoxin gene(Vrmlt63) has base sequence of SEQ ID NO:1. An amino acid sequence encoding low-temperature resistant proteins is indicated as SEQ ID NO:2. The 2-cysteine peroxiredoxin gene is induced by wound stresses, low temperature and oxidation. The 2-cysteine peroxiredoxin gene includes amino acid sequence of SEQ ID NO:2. The proteins induced by the gene have a function of antioxidant enzyme. The 2-cysteine peroxiredoxin is well-known for removing active oxygen.
Abstract translation: 提供了一种凋亡相关的2-半胱氨酸过氧氧化还原酶基因（Vrmlt63），其从绿豆分离并由应激表达。 2-半胱氨酸过氧化物毒素基因（Vrmlt63）具有SEQ ID NO：1的碱基序列。 编码耐低温蛋白质的氨基酸序列如SEQ ID NO：2所示。 2-半胱氨酸过氧氧化还原酶基因由伤口应激，低温和氧化诱导。 2-半胱氨酸过氧化氧化还原酶基因包括SEQ ID NO：2的氨基酸序列。 由该基因诱导的蛋白质具有抗氧化酶的功能。 2-半胱氨酸过氧氧化还原酶是用于去除活性氧的方法。
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公开(公告)号：KR1020090032676A
公开(公告)日：2009-04-01
申请号：KR1020070098109
申请日：2007-09-28
Applicant: 동아대학교 산학협력단
IPC: C07K14/195 , C12N15/31
Abstract: A gene cluster encoding antibiotic activity from metagenome of soil microbes is provided to produce antibiotic by transforming Escherichia coli, which has antifungal activity and antibacterial activity. A gene cluster encoding antibiotic activity includes orf20 to orf39 obtained from metagenome. A gene cluster from orf29 to orf36 directly involves expression of antibiotic activity. A gene cluster from orf20 to orf28 controls the expression of antibiotic activity genes. Proteins encoded by antibiotic activity genes from orf29 to orf36 have amino acid sequence of sequence number 4 to the sequence number 11. Proteins encoded by antibiotic activity genes from orf20 to orf28 have amino acid sequence of sequence number 2 and the sequence number 3.
Abstract translation: 通过转化具有抗真菌活性和抗菌活性的大肠杆菌，提供编码抗菌素活性的基因簇，通过土壤微生物巨噬细胞生成抗生素。 编码抗生素活性的基因簇包括从metagenome获得的orf20至orf39。 来自orf29至orf36的基因簇直接涉及抗生素活性的表达。 来自orf20至orf28的基因簇控制抗生素活性基因的表达。 由orf29至orf36的抗生素活性基因编码的蛋白质具有序列号4至序列号11的氨基酸序列。由orf20至orf28的抗生素活性基因编码的蛋白质具有序列号2和序列号3的氨基酸序列。
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