公开(公告)号：KR1020020068148A

公开(公告)日：2002-08-27

申请号：KR1020010008424

申请日：2001-02-20

Applicant: 동화약품주식회사

Inventor： 황순봉 ,  정용호 ,  유제만 ,  이경재 ,  정숙희 ,  성승규

IPC: C12N15/866

CPC classification number: C12N15/866 ,  C07K1/22 ,  C07K14/005 ,  C07K2319/00 ,  C12N5/0601 ,  C12N2730/10134 ,  C12N2760/10134

Abstract: PURPOSE: Provided is a divalent vaccine against hepatitis B and hepatitis D and a method for preparing it in high yield, thereby preventing hepatitis B and hepatitis D simultaneously. CONSTITUTION: Baculovirus transition vector, pVLHBs(WT)-HDs, contains HBV surface antigen coding gene having 158-836 nt region of SEQ ID NO:1 and HDV delta antigen coding gene having 1601-1016 nt region of SEQ ID NO:7. Recombinant baculovirus is prepared by transfection with the baculovirus transition vector. The divalent vaccine is expressed in a transfected insect cells by recombinant baculovirus.

Abstract translation: 目的：提供一种针对乙型肝炎和丙型肝炎的二价疫苗及其高产率制备方法，从而同时预防乙肝和丙型肝炎。 构成：杆状病毒转移载体pVLHB（WT）-HDs含有具有SEQ ID NO：1具有158-836nt区域的HBV表面抗原编码基因和具有SEQ ID NO：7的1601-1016nt区域的HDVδ抗原编码基因。 通过用杆状病毒转染载体转染制备重组杆状病毒。 二价疫苗通过重组杆状病毒在转染的昆虫细胞中表达。

公开(公告)号：KR100454867B1

公开(公告)日：2004-11-03

申请号：KR1020010008424

申请日：2001-02-20

Applicant: 동화약품주식회사

Inventor： 황순봉 ,  정용호 ,  유제만 ,  이경재 ,  정숙희 ,  성승규

IPC: C12N15/866

Abstract: PURPOSE: Provided is a divalent vaccine against hepatitis B and hepatitis D and a method for preparing it in high yield, thereby preventing hepatitis B and hepatitis D simultaneously. CONSTITUTION: Baculovirus transition vector, pVLHBs(WT)-HDs, contains HBV surface antigen coding gene having 158-836 nt region of SEQ ID NO:1 and HDV delta antigen coding gene having 1601-1016 nt region of SEQ ID NO:7. Recombinant baculovirus is prepared by transfection with the baculovirus transition vector. The divalent vaccine is expressed in a transfected insect cells by recombinant baculovirus.

Abstract translation: 目的：提供一种抗乙型肝炎和丁型肝炎的二价疫苗及其高产率制备方法，由此同时预防乙型肝炎和丁型肝炎。 构成：杆状病毒转移载体pVLHBs（WT）-HDs包含具有SEQ ID NO：1的158-836nt区域的HBV表面抗原编码基因和具有SEQ ID NO：7的1601-1016nt区域的HDVδ抗原编码基因。 通过用杆状病毒转移载体转染来制备重组杆状病毒。 二价疫苗通过重组杆状病毒在转染的昆虫细胞中表达。