公开(公告)号：KR100434117B1

公开(公告)日：2004-06-04

申请号：KR1020000065270

申请日：2000-11-03

Applicant: 동화약품주식회사

Inventor： 황순봉 ,  윤성준 ,  김종우 ,  이경재 ,  황연하 ,  윤승원

IPC: C12N7/01

Abstract: PURPOSE: Provided are a detecting method of hepatitis C virus(HCV) using recombinant mixed antigens derived from HCV and a diagnostic kit thereof, particularly by immuno chromatography using recombinant mixed antigens including core, NS3 and NS5A proteins of HCV gene as main components. CONSTITUTION: A recombinant Baculovirus(KCCM 10218) is manufactured by cotransfection of Baculovirus transfer vector including HCV derived core protein gene. HCV derived core protein is expressed from the recombinant Baculovirus(KCCM 10218) and has amino acid sequence of SEQ ID NO: 8. A recombinant Baculovirus(KCCM 10219) is manufactured by cotransfection of Baculovirus transfer vector including HCV derived NS3 protein gene. HCV derived NS3 proteins expressed from the recombinant Baculovirus(KCCM 10219) and has amino acid sequence of SEQ ID NO:9. An E.coli transformant(KCCM 10217) is transformed with a recombinant expression vector including HCV derived NS5A protein gene. HCV derived NS5A protein is expressed from the transformant and has amino acid sequence of SEQ ID NO: 10. The HCV diagnostic kit contains a mixed antigen consisting of core, NS3 and NS5A proteins derived from HCV.

Abstract translation: 目的：提供一种使用来源于HCV的重组混合抗原及其诊断试剂盒，特别是通过使用重组混合抗原（包括HCV基因的核心，NS3和NS5A蛋白）作为主要成分的免疫层析的丙型肝炎病毒（HCV）的检测方法。 构成：重组杆状病毒（KCCM10218）通过共转染包括HCV衍生的核心蛋白基因的杆状病毒转移载体来制造。 HCV衍生的核心蛋白由重组杆状病毒（KCCM 10218）表达并具有SEQ ID NO：8的氨基酸序列。通过共转染包括HCV衍生的NS3蛋白基因的杆状病毒转移载体来生产重组杆状病毒（KCCM 10219）。 由重组杆状病毒（KCCM 10219）表达的HCV衍生的NS3蛋白，具有SEQ ID NO：9的氨基酸序列。 用包含HCV衍生的NS5A蛋白质基因的重组表达载体转化大肠杆菌转化体（KCCM10217）。 HCV衍生的NS5A蛋白由转化体表达并具有SEQ ID NO：10的氨基酸序列。HCV诊断试剂盒含有由HCV衍生的核心，NS3和NS5A蛋白组成的混合抗原。

公开(公告)号：KR100454867B1

公开(公告)日：2004-11-03

申请号：KR1020010008424

申请日：2001-02-20

Applicant: 동화약품주식회사

Inventor： 황순봉 ,  정용호 ,  유제만 ,  이경재 ,  정숙희 ,  성승규

IPC: C12N15/866

Abstract: PURPOSE: Provided is a divalent vaccine against hepatitis B and hepatitis D and a method for preparing it in high yield, thereby preventing hepatitis B and hepatitis D simultaneously. CONSTITUTION: Baculovirus transition vector, pVLHBs(WT)-HDs, contains HBV surface antigen coding gene having 158-836 nt region of SEQ ID NO:1 and HDV delta antigen coding gene having 1601-1016 nt region of SEQ ID NO:7. Recombinant baculovirus is prepared by transfection with the baculovirus transition vector. The divalent vaccine is expressed in a transfected insect cells by recombinant baculovirus.

Abstract translation: 目的：提供一种抗乙型肝炎和丁型肝炎的二价疫苗及其高产率制备方法，由此同时预防乙型肝炎和丁型肝炎。 构成：杆状病毒转移载体pVLHBs（WT）-HDs包含具有SEQ ID NO：1的158-836nt区域的HBV表面抗原编码基因和具有SEQ ID NO：7的1601-1016nt区域的HDVδ抗原编码基因。 通过用杆状病毒转移载体转染来制备重组杆状病毒。 二价疫苗通过重组杆状病毒在转染的昆虫细胞中表达。

公开(公告)号：KR1020020034759A

公开(公告)日：2002-05-09

申请号：KR1020000065270

申请日：2000-11-03

Applicant: 동화약품주식회사

Inventor： 황순봉 ,  윤성준 ,  김종우 ,  이경재 ,  황연하 ,  윤승원

IPC: C12N7/01

CPC classification number: C12N7/00 ,  C12N2710/14011 ,  C12Q1/68

Abstract: PURPOSE: Provided are a detecting method of hepatitis C virus(HCV) using recombinant mixed antigens derived from HCV and a diagnostic kit thereof, particularly by immuno chromatography using recombinant mixed antigens including core, NS3 and NS5A proteins of HCV gene as main components. CONSTITUTION: A recombinant Baculovirus(KCCM 10218) is manufactured by cotransfection of Baculovirus transfer vector including HCV derived core protein gene. HCV derived core protein is expressed from the recombinant Baculovirus(KCCM 10218) and has amino acid sequence of SEQ ID NO: 8. A recombinant Baculovirus(KCCM 10219) is manufactured by cotransfection of Baculovirus transfer vector including HCV derived NS3 protein gene. HCV derived NS3 proteins expressed from the recombinant Baculovirus(KCCM 10219) and has amino acid sequence of SEQ ID NO:9. An E.coli transformant(KCCM 10217) is transformed with a recombinant expression vector including HCV derived NS5A protein gene. HCV derived NS5A protein is expressed from the transformant and has amino acid sequence of SEQ ID NO: 10. The HCV diagnostic kit contains a mixed antigen consisting of core, NS3 and NS5A proteins derived from HCV.

Abstract translation: 目的：提供使用HCV衍生的重组混合抗原的丙型肝炎病毒（HCV）的检测方法及其诊断试剂盒，特别是使用包含HCV基因的核心，NS3和NS5A蛋白的重组混合抗原的免疫色谱作为主要成分。 构成：通过共转染包括HCV衍生的核心蛋白基因的杆状病毒转移载体来制备重组杆状病毒（KCCM 10218）。 HCV衍生的核心蛋白由重组杆状病毒（KCCM 10218）表达并具有SEQ ID NO：8的氨基酸序列。通过共转染包含HCV衍生的NS3蛋白基因的杆状病毒转移载体来制备重组杆状病毒（KCCM 10219）。 由重组杆状病毒（KCCM 10219）表达的HCV衍生的NS3蛋白，具有SEQ ID NO：9的氨基酸序列。 用包含HCV衍生的NS5A蛋白基因的重组表达载体转化大肠杆菌转化体（KCCM 10217）。 HCV衍生的NS5A蛋白由转化体表达，并具有SEQ ID NO：10的氨基酸序列。HCV诊断试剂盒含有由核心，NS3和源自HCV的NS5A蛋白组成的混合抗原。

公开(公告)号：KR1020020068148A

公开(公告)日：2002-08-27

申请号：KR1020010008424

申请日：2001-02-20

Applicant: 동화약품주식회사

Inventor： 황순봉 ,  정용호 ,  유제만 ,  이경재 ,  정숙희 ,  성승규

IPC: C12N15/866

CPC classification number: C12N15/866 ,  C07K1/22 ,  C07K14/005 ,  C07K2319/00 ,  C12N5/0601 ,  C12N2730/10134 ,  C12N2760/10134

Abstract: PURPOSE: Provided is a divalent vaccine against hepatitis B and hepatitis D and a method for preparing it in high yield, thereby preventing hepatitis B and hepatitis D simultaneously. CONSTITUTION: Baculovirus transition vector, pVLHBs(WT)-HDs, contains HBV surface antigen coding gene having 158-836 nt region of SEQ ID NO:1 and HDV delta antigen coding gene having 1601-1016 nt region of SEQ ID NO:7. Recombinant baculovirus is prepared by transfection with the baculovirus transition vector. The divalent vaccine is expressed in a transfected insect cells by recombinant baculovirus.

Abstract translation: 目的：提供一种针对乙型肝炎和丙型肝炎的二价疫苗及其高产率制备方法，从而同时预防乙肝和丙型肝炎。 构成：杆状病毒转移载体pVLHB（WT）-HDs含有具有SEQ ID NO：1具有158-836nt区域的HBV表面抗原编码基因和具有SEQ ID NO：7的1601-1016nt区域的HDVδ抗原编码基因。 通过用杆状病毒转染载体转染制备重组杆状病毒。 二价疫苗通过重组杆状病毒在转染的昆虫细胞中表达。