Abstract:
An example of the present invention relates to a microorganism with improved lactic acid productivity, which improves the lactic acid productivity when overexpressing a lactic acid transporter which is a protein transporting a lactic acid to the inside or the outside of the cell. Moreover, the lactic acid productivity is improved when the activity of a lactic acid oxidase is inhibited and the activity of pyruvate deoxidant enzyme. A lactic acid production yield can be improved by using the transformed microorganism manufactured by the same, thereby being capable of useful industrial application.
Abstract:
The present invention relates to: a promoter specific homologous part which is homologous with a part of the promoter of a target gene; a marker gene which is connected with the promoter specific homologous part in order to be able to operate; a cassette, for causing a defect of the target gene, which is connected to the downstream of the marker gene and includes a gene specific homologous part which is homologous with at least one target gene; and a gene manipulation method which uses the cassette. The efficient gene manipulation and selection of a cell are possible by using the cassette and the gene manipulation method using the cassette.