Abstract:
일 양상에 따른 숙시닐-코에이:아세틸 코에이 트랜스퍼라제 또는 이의 변이체를 코딩하는 폴리뉴클레오티드, 3-옥소아디필-코에이 트랜스퍼라제 또는 이의 변이체를 코딩하는 폴리뉴클레오티드, ADH6 단백질 또는 이의 변이체를 코딩하는 폴리뉴클레오티드, BDHA 단백질 또는 이의 변이체를 코딩하는 폴리뉴클레오티드, 4HBD 단백질 또는 이의 변이체를 코딩하는 폴리뉴클레오티드 및 ALD5 단백질 또는 이의 변이체를 코딩하는 폴리뉴클레오티드를 포함하는 미생물은 외래 유전자의 도입으로 새로운 대사 경로를 갖게 되어 아디페이트를 효과적으로 생산할 수 있게 되었다. 이러한 형질전환된 미생물은 화학합성에 비하여 효율적으로 아디페이트를 생산할 수 있으므로 산업적으로 유용하게 이용할 수 있다.
Abstract:
Provided are a novel promoter of Corynebacterium glutamicum; a vector including the promoter; a host cell having the vector; and a method to express gene using the host cell. When comparing and analyzing the expression of the promoter with a conventional gapA promoter, high expression level is maintained not only under an aerobic condition but an anaerobic condition. Construction of a system which can overexpress desired genes in the Corynebacterium glutamicum using the promoter can be possible.
Abstract:
PURPOSE: A deformed microorganism for production of 1,4-butanediol is provided to produce 1,4-BDO by a biological producing process. CONSTITUTION: A deformed microorganism for production of 1.4-butanediol comprises: an activation converting alpha-ketoglutarate or succinate into 4-hydroxybutyril-CoA ("4-HB-CoA"); and an activation converting the 4-HB-CoA into 1,4-butanediols ("1,4-BDO"). The activation converting the 4-HB-CoA into the 1,4-BDO is coded by more than one kind of genes selected from a group comprising adh1, yiaY, adh4, adhB, mdh, eutG, fucO, dhaT, aldA, eutE, adhE1, adhE2 and adh2.
Abstract:
PURPOSE: A solid lipid nanoparticle is provided to offer an effective method for transferring an active ingredient to the target site of body. CONSTITUTION: A solid lipid nanoparticle comprises an elastin-like polypeptide in which the moiety having more than one hydrophobic groups are bound and lipid molecule. The moiety having hydrophobic group is the saturated or unsaturated hydrocarbon group, the saturated or unsaturated acyl group, the substitute amide group having a chemical formula -C(CO)N(R1)(R2), which means the R1 and R2 are the respectively independent saturated or unsaturated hydrocarbon, or the saturated or unsaturated alkoxy group. The lipid molecule is neutral lipid molecule or amphiphilic lipid molecule. The elastic-like polypeptide includes more than one repeat unit selected from among VPGXG, PGXGV, GXGVP, XGVPG, GVPGX, and a group comprised of combination thoseof more than once. The V, P, G, and X is respectively means valine, proline, glycine, and an amino acid which is not proline. [Reference numerals] (AA) Coefficient
Abstract:
PURPOSE: A high throughput screening system and a method of lactic acid-producing strains using two or more pH indicators are provided to quickly and accurately screen strains. CONSTITUTION: A high throughput screening system of lactic acid-producing strains contains the strain, a medium, and two or more pH indicators. The strains are a wild type strain, a mutant strain, and a recombinant strain. The pH indicators contain bromocresol green and methyl red in a ratio of 2:1-10:1. A method for screening the lactic acid-producing strains comprises: a step of adding the pH indicators to a medium; a step of culturing the strains; and a step of observing color change of the pH indicators, and measuring lactic acid production quantity.
Abstract:
PURPOSE: A method for preparing 3-HP(3-hydroxypropionic acid) and 1,3-PDO(1,3-propandiol) using microorganisms is provided to convert a glycerol substrate into 3-HP and 1,3-PDO. CONSTITUTION: A recombinant microorganism for preparing 3-HP and 1,3-PDO contains genes producing the compounds. The genes are dhaB(glycerol dehydratease), alhH(aldehyde dehydrogenase), and dhaT(1,3-propandiol oxidoreductase). dhaB gene is derived from K. pneumoniae or C. butyricum. alhH gene is derived from E.coli. The recombinant microorganism is E.coli. A method for producing 3-HP and 1,3-PDO comprises a step of culturing the recombinant microorganism in a medium containing a carbon substrate. The carbon substrate is glucose, sucrose, cellulose, or glycerol.
Abstract:
PURPOSE: A method for producing bio alcohol using a polynucleotide which enhances alcohol resistance of host cells is provided to enhance productivity in alcohol fermentation. CONSTITUTION: An isolated polynucleotide comprises: a polynucleotide comprising nucleotide having 90% or more identity with a nucleotide selected from sequence numbers 1-8; a polynucleotide encoding a polypeptide comprising amino acids having 90% or more identity with amino acids selected from sequence numbers 14-20; a polynucleotide comprising nucleotide sequence hybridizing with the selected nucleotide sequence under severe condition; and a polypeptide encoding polypeptide.
Abstract:
PURPOSE: A sugar-derived cyclic acetal monomer from sorbitol or gluconic acid is provided to produce a raw material having biodegradable characteristic and to apply to electricity/electronic housing. CONSTITUTION: A sugar-derived cylcic acetal monomer is denoted by chemical formula 1 or 2. A method for preparing the sugar-derived cylcic acetal monomer comprises: a step of substituting hydroxy group of D-sorbitol terminal with ester; a step of step of converting secondary alcohol group of the resultant to cyclic acetal structure using aldehyde or ketone; and a step of removing ester group from ester-substituted terminal group to reduce to free alcohol.