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公开(公告)号：KR1020130000688A
公开(公告)日：2013-01-03
申请号：KR1020110061366
申请日：2011-06-23
Applicant: 삼성전자주식회사
Abstract: PURPOSE: A high throughput screening system and a method of lactic acid-producing strains using two or more pH indicators are provided to quickly and accurately screen strains. CONSTITUTION: A high throughput screening system of lactic acid-producing strains contains the strain, a medium, and two or more pH indicators. The strains are a wild type strain, a mutant strain, and a recombinant strain. The pH indicators contain bromocresol green and methyl red in a ratio of 2:1-10:1. A method for screening the lactic acid-producing strains comprises: a step of adding the pH indicators to a medium; a step of culturing the strains; and a step of observing color change of the pH indicators, and measuring lactic acid production quantity.
Abstract translation: 目的：提供高通量筛选系统和使用两种或多种pH指示剂的乳酸生产菌株的方法，以快速准确筛选菌株。 构成：乳酸生产菌株的高通量筛选系统含有菌株，培养基和两种或多种pH指示剂。 菌株是野生型菌株，突变菌株和重组菌株。 pH指标含有溴甲酚绿和甲基红，比例为2：1-10：1。 筛选乳酸生产菌株的方法包括：将pH指示剂加入到培养基中的步骤; 培养菌株的一个步骤; 以及观察pH指示剂的变色，测定乳酸生成量的工序。
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公开(公告)号：KR1020120099315A
公开(公告)日：2012-09-10
申请号：KR1020110007877
申请日：2011-01-26
Applicant: 삼성전자주식회사
CPC classification number: C12Y402/0103 , C12N9/0006 , C12N9/0008 , C12N9/88 , C12P7/18 , C12P7/42 , C12Y101/01202 , C12Y102/01
Abstract: PURPOSE: A method for preparing 3-HP(3-hydroxypropionic acid) and 1,3-PDO(1,3-propandiol) using microorganisms is provided to convert a glycerol substrate into 3-HP and 1,3-PDO. CONSTITUTION: A recombinant microorganism for preparing 3-HP and 1,3-PDO contains genes producing the compounds. The genes are dhaB(glycerol dehydratease), alhH(aldehyde dehydrogenase), and dhaT(1,3-propandiol oxidoreductase). dhaB gene is derived from K. pneumoniae or C. butyricum. alhH gene is derived from E.coli. The recombinant microorganism is E.coli. A method for producing 3-HP and 1,3-PDO comprises a step of culturing the recombinant microorganism in a medium containing a carbon substrate. The carbon substrate is glucose, sucrose, cellulose, or glycerol.
Abstract translation: 目的：提供使用微生物制备3-HP（3-羟基丙酸）和1,3-PDO（1,3-丙二醇）的方法，以将甘油底物转化为3-HP和1,3-PDO。 构成：用于制备3-HP和1,3-PDO的重组微生物含有产生该化合物的基因。 基因是dhaB（甘油脱水酶），alhH（醛脱氢酶）和dhaT（1,3-丙二醇氧化还原酶）。 dhaB基因衍生自肺炎克雷伯杆菌或C.丁酸菌。 alhH基因来自大肠杆菌。 重组微生物是大肠杆菌。 制备3-HP和1,3-PDO的方法包括在含有碳底物的培养基中培养重组微生物的步骤。 碳底物是葡萄糖，蔗糖，纤维素或甘油。
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3.发明公开호스트셀의 알코올 내성을 증대시키는 단리된 폴리뉴클레오티드, 이를 포함하는 벡터, 호스트셀 및 이를 이용한 알코올의 생산방법 无效分离多元醇增加酒精耐受性，载体和含有它的宿主细胞，以及使用其制造酒精的方法
公开(公告)号：KR1020100117465A
公开(公告)日：2010-11-03
申请号：KR1020090036253
申请日：2009-04-24
Applicant: 삼성전자주식회사 , 성균관대학교산학협력단
CPC classification number: C07K14/395
Abstract: PURPOSE: A method for producing bio alcohol using a polynucleotide which enhances alcohol resistance of host cells is provided to enhance productivity in alcohol fermentation. CONSTITUTION: An isolated polynucleotide comprises: a polynucleotide comprising nucleotide having 90% or more identity with a nucleotide selected from sequence numbers 1-8; a polynucleotide encoding a polypeptide comprising amino acids having 90% or more identity with amino acids selected from sequence numbers 14-20; a polynucleotide comprising nucleotide sequence hybridizing with the selected nucleotide sequence under severe condition; and a polypeptide encoding polypeptide.
Abstract translation: 目的：提供使用增强宿主细胞的耐酒精性的多核苷酸生产生物醇的方法，以提高酒精发酵的生产率。 构成：分离的多核苷酸包含：包含与选自序列号1-8的核苷酸具有90％或更高同一性的核苷酸的多核苷酸; 编码多肽的多核苷酸，其包含与选自序列号14-20的氨基酸具有90％以上同一性的氨基酸; 包含在恶劣条件下与选择的核苷酸序列杂交的核苷酸序列的多核苷酸; 和编码多肽的多肽。
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公开(公告)号：KR1020110110999A
公开(公告)日：2011-10-10
申请号：KR1020100030357
申请日：2010-04-02
Applicant: 삼성전자주식회사
Abstract: 바이오매스 또는 다당류로부터 직접 알코올을 생산하는 균주로서 사카로파구스 데그라단스를 이용하는 기술을 제공한다. 이와 같은 균주를 이용하는 경우 알코올의 제조 공정 비용 및 시간을 크게 단축시킬 수 있어서 산업적으로 유용하다.
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公开(公告)号：KR1020110010346A
公开(公告)日：2011-02-01
申请号：KR1020090067863
申请日：2009-07-24
Applicant: 삼성전자주식회사
CPC classification number: C12P19/14 , C12N1/20 , C12N9/88 , C12P19/02 , C12Y402/02011 , Y02E50/343
Abstract: PURPOSE: A method for preparing bio-fuel using brown algae is provided to effectively saccharify brown algae and use in producing industrial bio-fuel. CONSTITUTION: A bio-fuel is prepared by adding hydrolyze catalyst of Bacterium Antarctica, culture liquid of Bacterium Antarctica, supernatant of the culture liquid, or pulverized liquid of the culture liquid to brown algae through sacchificiation. The brown algae is laminaria japonica, gulfweed, ecklonia cava, rhei Rhizoma, or sargassum thunbergii. The Bacterium Antarctica contains 16S rRNA having 95% of homology with a sequence corresponding to Bacterium antarctica AL-1(deposit number KCTC 11531 BP). A base sequence of 16S rRNA has sequence number 1.
Abstract translation: 目的：提供使用棕色藻类制备生物燃料的方法，以有效地糖化褐藻，并用于生产工业生物燃料。 构成：通过将南极洲的水解催化剂，南极洲细菌的培养液，培养液的上清液或培养液的粉碎液通过糖化将棕色藻类加入生物燃料中制备生物燃料。 棕色藻类是lamin粳，草甘膦，ecklonia cava，rhei Rhizoma或sargassum thunbergii。 南极洲细菌含有与对应于南极洲AL-1（保藏号KCTC 11531BP）的序列具有95％同源性的16S rRNA。 16S rRNA的碱基序列具有序列号1。
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Patent Agency Ranking
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公开(公告)号：KR101690284B1
公开(公告)日：2016-12-28
申请号：KR1020100041570
申请日：2010-05-03
Applicant: 삼성전자주식회사 , 성균관대학교산학협력단
CPC classification number: Y02E50/17
Abstract: 알코올발효균주로서갈락토오스대사능을증대시키는폴리펩티드및 알코올내성폴리펩티드가동시에과발현되거나, 호흡결손변이된재조합균주를제공한다. 이와같은균주를이용하는경우갈락토오스대사능및 알코올내성이향상되어알코올생산성이우수하므로산업적으로유용하다.
Abstract translation: 目的：提供含有截短的Tup1多肽的重组菌株和含有其的用于生产醇的组合物以改善耐酒精性并确保酒精生产率。 构成：重组菌株含有具有表达抑制结构域的功能的第一多肽从Tup1截短，其中表达有Ino1的第二多肽。 第一多肽含有氨基酸序列，其中288-389位氨基酸中的一个或多个被取代，插入，添加或截短。 第二多肽具有与序列号2的氨基酸具有70％以上序列同源性的氨基酸序列。羧基末端结构域是截短的Tup1多肽。 用于生产醇的组合物含有重组链。
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公开(公告)号：KR1020130001509A
公开(公告)日：2013-01-04
申请号：KR1020110062312
申请日：2011-06-27
Applicant: 삼성전자주식회사
CPC classification number: C12P7/42 , C12N9/0006 , C12N9/1029 , C12Y101/01001 , C12Y101/01027 , C12Y203/01008
Abstract: PURPOSE: A recombinant microorganism which produces 3-hydroxypropionic acid(3-HP) using a malonic semialdehyde reduction pathway is provided to remarkably enhance 3-HP productivity by gene manipulation. CONSTITUTION: A recombinant microorganism for producing 3-hydroxypropionic acid has a pathway for sequentially producing pyruvate, acetyl Co, malonyl-CoA, and malonic semialdehyde. The microorganism includes Escherichia sp., Saccharomyces sp., or Kluyveromyces sp. A gene encoding lactate dehydrogenase is IdhA or a homolog or a variant thereof. A gene encoding phosphotransacetylase is pta or a homolog or a variant thereof.
Abstract translation: 目的：提供使用丙二醛还原途径产生3-羟基丙酸（3-HP）的重组微生物，通过基因操作显着提高3-HP生产力。 构成：用于产生3-羟基丙酸的重组微生物具有顺序产生丙酮酸，乙酰辅酶A，丙二酰辅酶A和丙二醛半醛的途径。 微生物包括大肠杆菌，酵母属或克鲁维酵母属。 编码乳酸脱氢酶的基因是IdhA或其同源物或其变体。 编码磷酸转乙酰酶的基因是pta或其同系物或其变体。
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公开(公告)号：KR1020130000883A
公开(公告)日：2013-01-03
申请号：KR1020110061677
申请日：2011-06-24
Applicant: 삼성전자주식회사 , 성균관대학교산학협력단
CPC classification number: C12N15/815 , C12P21/00 , C12P21/02
Abstract: PURPOSE: An expression vector which overexpresses target proteins, and a method for producing the target protein are provided to obtain the proteins in K. marxianus. CONSTITUTION: An expression vector contains: a replication origin; a CYC promoter, a TEF promoter, a GPD promoter, or an ADH promoter; and a terminator. The CYC promoter contains a sequence of sequence number 1 or a sequence having 70% or more sequence homology with the sequence of sequence number 1. The TEF promoter has a sequence of sequence number 2 or a sequence with 70% or more sequence homology with the sequence of sequence number 2. GPD promoter contains a sequence of sequence number 3 or a sequence having 70% or more homology with the sequence of sequence number 3. The ADH promoter has a sequence of sequence number 4 or a sequence having 70% or more sequence homology with the sequence of sequence number 4.
Abstract translation: 目的：提供过表达靶蛋白的表达载体和产生靶蛋白的方法，以获得马克斯马氏霉属中的蛋白质。 构成：表达载体包含：复制起点; CYC启动子，TEF启动子，GPD启动子或ADH启动子; 和终结者。 CYC启动子含有序列号1的序列或与序列号1的序列具有70％以上序列同源性的序列.TEF启动子具有序列号2的序列或具有70％以上序列同源性的序列 序列号2的序列.GPD启动子含有序列号3的序列或与序列号3的序列具有70％以上同源性的序列.ADH启动子具有序列号4的序列或70％以上的序列 与序列号4的序列同源性。
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公开(公告)号：KR1020130000640A
公开(公告)日：2013-01-03
申请号：KR1020110061290
申请日：2011-06-23
Applicant: 삼성전자주식회사
CPC classification number: G01N21/80 , C12Q1/045 , Y02A50/451
Abstract: PURPOSE: A high throughput screening system and method using a pH indicator are provided to accurately, quickly, and easily screen strains which highly produce acids. CONSTITUTION: A system for screening acid-producing stains contains the acid-producing strains, a medium, two or more pH indicators. The acid includes citric acid, itaconic acid, succinic acid, fumaric acid, glycolic acid, pyruvic acid, acetic acid, glutamic acid, malic acid, maleic acid, 3-hydroxypropionic acid(3-HP), butyric acid, or gluconic acid. The strains are Bacillus sp., Streptococcus sp., Streptomyces sp., Staphylococcus sp., Enterococcus sp., Lactobacillus sp., Lactococcus sp., Clostridium sp., Geobacillus sp., Escherichia. Coli sp., Pseudomonas sp., Salmonella sp., Campylobacter sp., Helicobacter sp., Flavobactenum sp., Fusobacterium sp., llyobacter sp., Neisseria sp., Candida sp., Hansenula sp., Kluyveromyces sp., Pichia sp., Saccharomyces sp., Schizosaccharomyces sp., or Yarrowta sp.
Abstract translation: 目的：提供高通量筛选系统和使用pH指示剂的方法来准确，快速，容易地筛选高度产生酸的菌株。 构成：用于筛选产酸污渍的系统包含酸生产菌株，中等，两种或更多种pH指示剂。 酸包括柠檬酸，衣康酸，琥珀酸，富马酸，乙醇酸，丙酮酸，乙酸，谷氨酸，苹果酸，马来酸，3-羟基丙酸（3-HP），丁酸或葡萄糖酸。 菌株是芽孢杆菌属，链球菌属，链霉菌属，葡萄球菌属，肠球菌属，乳杆菌属，乳球菌属，梭菌属，地芽孢杆菌属，埃希氏菌属。 大肠杆菌，假单胞菌属，沙门氏菌属，弯曲菌属，幽门螺杆菌属，黄杆菌属，福氏杆菌属，丝杆菌属，奈瑟氏球菌属，假丝酵母属，汉逊酵母属，克鲁维酵母菌属，毕赤酵母属 ，酵母属（Saccharomyces sp。），粟酒裂殖酵母属（Schizosaccharomyces sp。）或Yarrowta sp。
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