公开(公告)号：KR1020100117465A

公开(公告)日：2010-11-03

申请号：KR1020090036253

申请日：2009-04-24

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 유병조 ,  박재찬 ,  박성민 ,  권대혁 ,  홍민의

IPC: C12N15/31 ,  C12N15/63 ,  C12P7/04 ,  C12R1/865

CPC classification number: C07K14/395

Abstract: PURPOSE: A method for producing bio alcohol using a polynucleotide which enhances alcohol resistance of host cells is provided to enhance productivity in alcohol fermentation. CONSTITUTION: An isolated polynucleotide comprises: a polynucleotide comprising nucleotide having 90% or more identity with a nucleotide selected from sequence numbers 1-8; a polynucleotide encoding a polypeptide comprising amino acids having 90% or more identity with amino acids selected from sequence numbers 14-20; a polynucleotide comprising nucleotide sequence hybridizing with the selected nucleotide sequence under severe condition; and a polypeptide encoding polypeptide.

Abstract translation: 目的：提供使用增强宿主细胞的耐酒精性的多核苷酸生产生物醇的方法，以提高酒精发酵的生产率。 构成：分离的多核苷酸包含：包含与选自序列号1-8的核苷酸具有90％或更高同一性的核苷酸的多核苷酸; 编码多肽的多核苷酸，其包含与选自序列号14-20的氨基酸具有90％以上同一性的氨基酸; 包含在恶劣条件下与选择的核苷酸序列杂交的核苷酸序列的多核苷酸; 和编码多肽的多肽。

公开(公告)号：KR101690284B1

公开(公告)日：2016-12-28

申请号：KR1020100041570

申请日：2010-05-03

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 유병조 ,  박성민 ,  권대혁 ,  이기성

IPC: C12P7/06 ,  C12R1/865 ,  C12N15/81

CPC classification number: Y02E50/17

Abstract: 알코올발효균주로서갈락토오스대사능을증대시키는폴리펩티드및 알코올내성폴리펩티드가동시에과발현되거나, 호흡결손변이된재조합균주를제공한다. 이와같은균주를이용하는경우갈락토오스대사능및 알코올내성이향상되어알코올생산성이우수하므로산업적으로유용하다.

Abstract translation: 目的：提供含有截短的Tup1多肽的重组菌株和含有其的用于生产醇的组合物以改善耐酒精性并确保酒精生产率。 构成：重组菌株含有具有表达抑制结构域的功能的第一多肽从Tup1截短，其中表达有Ino1的第二多肽。 第一多肽含有氨基酸序列，其中288-389位氨基酸中的一个或多个被取代，插入，添加或截短。 第二多肽具有与序列号2的氨基酸具有70％以上序列同源性的氨基酸序列。羧基末端结构域是截短的Tup1多肽。 用于生产醇的组合物含有重组链。

公开(公告)号：KR1020130000883A

公开(公告)日：2013-01-03

申请号：KR1020110061677

申请日：2011-06-24

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 구현민 ,  박성민 ,  유병조 ,  이기성 ,  박재찬 ,  권대혁

IPC: C12N15/70 ,  C12N15/81 ,  C12N15/67

CPC classification number: C12N15/815 ,  C12P21/00 ,  C12P21/02

Abstract: PURPOSE: An expression vector which overexpresses target proteins, and a method for producing the target protein are provided to obtain the proteins in K. marxianus. CONSTITUTION: An expression vector contains: a replication origin; a CYC promoter, a TEF promoter, a GPD promoter, or an ADH promoter; and a terminator. The CYC promoter contains a sequence of sequence number 1 or a sequence having 70% or more sequence homology with the sequence of sequence number 1. The TEF promoter has a sequence of sequence number 2 or a sequence with 70% or more sequence homology with the sequence of sequence number 2. GPD promoter contains a sequence of sequence number 3 or a sequence having 70% or more homology with the sequence of sequence number 3. The ADH promoter has a sequence of sequence number 4 or a sequence having 70% or more sequence homology with the sequence of sequence number 4.

Abstract translation: 目的：提供过表达靶蛋白的表达载体和产生靶蛋白的方法，以获得马克斯马氏霉属中的蛋白质。 构成：表达载体包含：复制起点; CYC启动子，TEF启动子，GPD启动子或ADH启动子; 和终结者。 CYC启动子含有序列号1的序列或与序列号1的序列具有70％以上序列同源性的序列.TEF启动子具有序列号2的序列或具有70％以上序列同源性的序列 序列号2的序列.GPD启动子含有序列号3的序列或与序列号3的序列具有70％以上同源性的序列.ADH启动子具有序列号4的序列或70％以上的序列 与序列号4的序列同源性。

公开(公告)号：KR1020100048224A

公开(公告)日：2010-05-11

申请号：KR1020080107277

申请日：2008-10-30

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 유병조 ,  박재찬 ,  구현민 ,  진용수 ,  이기성

IPC: C12N15/12 ,  C12N1/16 ,  C12N15/66

CPC classification number: C12P7/08 ,  C07K14/395 ,  Y02E50/17

Abstract: PURPOSE: A gene which enhances metabolic rate of galactose is provided to enhance productivity of bioalcohol from biomass. CONSTITUTION: A gene which enhances metabolic rate of galactose is formed by losing whole or partial expression suppressing domain in a regulatory gene which suppresses expression of galactose matobolic gene. The regulatory gene is a gene encoding a TUP1 protein. A domain suppressing expression of the gene is a C-terminal repressor domain. The regulatory gene has a polynucleotide sequence of sequence number 1. The TUP1 protein has an amino acid sequence of sequence number 2. A pRS424 recombinant vector contains the of sequence number 1. A transformed recombinant microorganism is prepared using the recombinant vector. The microorganism is yeast. The recombinant microorganism is Saccharomyces cerevisiae CEN.PK2-1D/pRS424-truncated TUP1 of deposit number KCTC 11387 BP.

Abstract translation: 目的：提高提高半乳糖代谢率的基因，以提高生物质中生物醇的生产力。 构成：通过在抑制半乳糖基质的表达的调节基因中失去全部或部分表达抑制结构域，形成提高半乳糖代谢率的基因。 调节基因是编码TUP1蛋白的基因。 抑制基因表达的结构域是C末端阻遏酶结构域。 调节基因具有序列号1的多核苷酸序列.TUP1蛋白具有序列号2的氨基酸序列.PRS424重组载体含有序列号1.使用重组载体制备转化的重组微生物。 微生物是酵母。 重组微生物是保藏号为KCTC 11387BP的酿酒酵母CEN.PK2-1D / pRS424-截短的TUP1。

公开(公告)号：KR101569210B1

公开(公告)日：2015-11-23

申请号：KR1020080106278

申请日：2008-10-29

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 유병조 ,  박재찬 ,  구현민 ,  진용수 ,  이기성

IPC: C12N15/52 ,  C12N15/56 ,  C12N15/09

CPC classification number: C12N15/1034 ,  C07K14/395 ,  C12P7/04 ,  C12P7/06 ,  C12P7/16 ,  C12P7/28 ,  Y02E50/10 ,  Y02E50/17

Abstract: 과발현시갈락토오스의대사이용성을증대시킬수 있는유전자에관한기술을제공한다. 구체적인예에서, 과발현시갈락토오스의대사이용성을증가시키는유전자로서, SNR84 유전자를제공한다. SNR84 유전자는과발현됨으로써갈락토오스의대사율을증가시킬수 있으므로, 갈락토오스를함유하는탄소원으로부터바이오알코올의생산성을크게증가시킬수 있다.

公开(公告)号：KR1020130124065A

公开(公告)日：2013-11-13

申请号：KR1020120047683

申请日：2012-05-04

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 유병조 ,  구현민 ,  박성민 ,  권대혁 ,  김재영 ,  박재찬

IPC: C12N1/19 ,  C12N15/55 ,  C12P7/06 ,  C12N15/81

CPC classification number: Y02E50/17 ,  C12N15/815 ,  C12N9/2402 ,  C12P7/10 ,  C12P7/40 ,  C12P13/04 ,  C12P19/02 ,  C12P19/04

Abstract: Disclosed is modified microorganisms for simultaneous saccharification and fermentation, an expression vector for preparing the modified microorganisms, and a method for producing chemical materials using the modified microorganisms. According to one side, disclosed is modified Kluyveromyces marxianus which produces chemical materials by simultaneous saccharification and fermentation and contains a replication origin; a promoter; a gene encodes one or more cellulose decomposing enzymes selected among beta-glucosidase, endoglucanase, exoglucanase, and cellobiohydrolase; and a terminator.

Abstract translation: 公开了用于同时糖化和发酵的改性微生物，用于制备改性微生物的表达载体，以及使用改性微生物生产化学物质的方法。 一方面，公开了通过同时进行糖化和发酵生产化学物质的马克斯克鲁维酵母（Kluyveromyces marxianus），并含有复制起点; 启动子 一种基因编码一种或多种选自β-葡糖苷酶，内切葡聚糖酶，外切葡聚糖酶和纤维二糖水解酶的纤维素分解酶; 和终结者。

公开(公告)号：KR1020110122012A

公开(公告)日：2011-11-09

申请号：KR1020100041570

申请日：2010-05-03

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 유병조 ,  박성민 ,  권대혁 ,  이기성

IPC: C12P7/06 ,  C12R1/865 ,  C12N15/81

CPC classification number: Y02E50/17 ,  C12P7/06 ,  C12N15/81 ,  C12R1/865 ,  Y10S435/942

Abstract: PURPOSE: A recombinant strain containing truncated Tup1 polypeptide and a composition containing the same for producing alcohol are provided to improve alcohol resistance and to ensure alcohol productivity. CONSTITUTION: A recombinant strain contains first polypeptides having the function of an expression inhibition domain is truncated from Tup1 and a second polypeptide in which Ino1 is expressed. The first polypeptide contains amino acid sequence in which one or more among 288-389th amino acids are substituted, inserted, added or truncated. The second polypeptide has an amino acid sequence having 70% or more sequence homology with an amino acid of sequence number 2. The carboxyl terminal end domain is a truncated Tup1 polypeptide. A composition for producing alcohol contains the recombinant strand.

Abstract translation: 目的：提供含有截短的Tup1多肽的重组菌株和含有其的用于生产醇的组合物以改善耐酒精性并确保酒精生产率。 构成：重组菌株含有具有表达抑制结构域的功能的第一多肽从Tup1截短，其中表达有Ino1的第二多肽。 第一多肽含有氨基酸序列，其中288-389位氨基酸中的一个或多个被取代，插入，添加或截短。 第二多肽具有与序列号2的氨基酸具有70％以上序列同源性的氨基酸序列。羧基末端结构域是截短的Tup1多肽。 用于生产醇的组合物含有重组链。

公开(公告)号：KR1020100047407A

公开(公告)日：2010-05-10

申请号：KR1020080106278

申请日：2008-10-29

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 유병조 ,  박재찬 ,  구현민 ,  진용수 ,  이기성

IPC: C12N15/52 ,  C12N15/56 ,  C12N15/09

CPC classification number: C12N15/1034 ,  C07K14/395 ,  C12P7/04 ,  C12P7/06 ,  C12P7/16 ,  C12P7/28 ,  Y02E50/10 ,  Y02E50/17 ,  C12N15/52 ,  C12N9/24 ,  C12N15/09

Abstract: PURPOSE: An SNR84 gene enhancing usability of galactose metabolism is provided to enhance metabolic rate of galactose and enhance productivity of bioalcohol from carbon source. CONSTITUTION: An SNR84 gene enhances galactose metabolism rate. The gene is denoted by sequence number 1. A pRS424 recombinant vector contains the SNR84 gene of sequence number 1. A transformed recombinant microorganism is obtained using the recombinant vector. The recombinant microorganism overexpresses the SNR84 gene. The microorganism is yeast. The yeast is Saccharomyces sp., Pachysolen sp., Clavispora sp., Kluyveromyces sp., Debaryomyces sp., Schwanniomyces sp., Candida sp., Pichia sp., or Dekkera sp. The deposit number KCTC 11388 BP of the recombinant microorganism is CEN.PK2-1D/pRS424-SNR84.

Abstract translation: 目的：提供增强半乳糖代谢可用性的SNR84基因，以提高半乳糖的代谢率，并提高生物醇从碳源的生产力。 构成：SNR84基因增强半乳糖代谢率。 该基因由序列号1表示.PRS424重组载体含有序列号1的SNR84基因。使用重组载体获得转化的重组微生物。 重组微生物过表达SNR84基因。 微生物是酵母。 酵母是酵母属（Saccharomyces sp。），Pachysolen sp。，Clavispora sp。，Kluyveromyces sp。，Debaryomyces sp。，Schwanniomyces sp。，Candida sp。，Pichia sp。或Dekkera sp。 重组微生物的保藏号KCTC 11388 BP为CEN.PK2-1D / pRS424-SNR84。

公开(公告)号：KR1020130000688A

公开(公告)日：2013-01-03

申请号：KR1020110061366

申请日：2011-06-23

Applicant: 삼성전자주식회사

Inventor： 조화영 ,  유병조 ,  박재찬 ,  박성민 ,  구현민 ,  이주영

IPC: C12Q1/04 ,  C12N1/20 ,  C12N1/14 ,  C12P7/56

CPC classification number: G01N21/80 ,  C12Q1/025

Abstract: PURPOSE: A high throughput screening system and a method of lactic acid-producing strains using two or more pH indicators are provided to quickly and accurately screen strains. CONSTITUTION: A high throughput screening system of lactic acid-producing strains contains the strain, a medium, and two or more pH indicators. The strains are a wild type strain, a mutant strain, and a recombinant strain. The pH indicators contain bromocresol green and methyl red in a ratio of 2:1-10:1. A method for screening the lactic acid-producing strains comprises: a step of adding the pH indicators to a medium; a step of culturing the strains; and a step of observing color change of the pH indicators, and measuring lactic acid production quantity.

Abstract translation: 目的：提供高通量筛选系统和使用两种或多种pH指示剂的乳酸生产菌株的方法，以快速准确筛选菌株。 构成：乳酸生产菌株的高通量筛选系统含有菌株，培养基和两种或多种pH指示剂。 菌株是野生型菌株，突变菌株和重组菌株。 pH指标含有溴甲酚绿和甲基红，比例为2：1-10：1。 筛选乳酸生产菌株的方法包括：将pH指示剂加入到培养基中的步骤; 培养菌株的一个步骤; 以及观察pH指示剂的变色，测定乳酸生成量的工序。

公开(公告)号：KR1020120099315A

公开(公告)日：2012-09-10

申请号：KR1020110007877

申请日：2011-01-26

Applicant: 삼성전자주식회사

Inventor： 박성민 ,  유병조 ,  김재영 ,  박영경 ,  박재찬 ,  조화영

IPC: C12N1/21 ,  C12N15/53 ,  C12N15/70 ,  C12P7/18

CPC classification number: C12Y402/0103 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/88 ,  C12P7/18 ,  C12P7/42 ,  C12Y101/01202 ,  C12Y102/01

Abstract: PURPOSE: A method for preparing 3-HP(3-hydroxypropionic acid) and 1,3-PDO(1,3-propandiol) using microorganisms is provided to convert a glycerol substrate into 3-HP and 1,3-PDO. CONSTITUTION: A recombinant microorganism for preparing 3-HP and 1,3-PDO contains genes producing the compounds. The genes are dhaB(glycerol dehydratease), alhH(aldehyde dehydrogenase), and dhaT(1,3-propandiol oxidoreductase). dhaB gene is derived from K. pneumoniae or C. butyricum. alhH gene is derived from E.coli. The recombinant microorganism is E.coli. A method for producing 3-HP and 1,3-PDO comprises a step of culturing the recombinant microorganism in a medium containing a carbon substrate. The carbon substrate is glucose, sucrose, cellulose, or glycerol.

Abstract translation: 目的：提供使用微生物制备3-HP（3-羟基丙酸）和1,3-PDO（1,3-丙二醇）的方法，以将甘油底物转化为3-HP和1,3-PDO。 构成：用于制备3-HP和1,3-PDO的重组微生物含有产生该化合物的基因。 基因是dhaB（甘油脱水酶），alhH（醛脱氢酶）和dhaT（1,3-丙二醇氧化还原酶）。 dhaB基因衍生自肺炎克雷伯杆菌或C.丁酸菌。 alhH基因来自大肠杆菌。 重组微生物是大肠杆菌。 制备3-HP和1,3-PDO的方法包括在含有碳底物的培养基中培养重组微生物的步骤。 碳底物是葡萄糖，蔗糖，纤维素或甘油。