公开(公告)号：KR1020120114468A

公开(公告)日：2012-10-17

申请号：KR1020110031979

申请日：2011-04-07

Applicant: 서울대학교산학협력단

Inventor： 최윤재 ,  김형기 ,  윤금룡 ,  김훈 ,  김준겸

IPC: C12N5/0775 ,  A61K35/28

CPC classification number: C12N5/0668 ,  A61K35/28

Abstract: PURPOSE: A mesenchymal stem cell in which endostatin and carboxyl esterase are concurrently over expressed and preventing, treating or recurrence suppression method of glioma by using the same are provided to drastically prevent carcinogenesis and recurrence. CONSTITUTION: In a mesenchymal stem cell, endostatin and carboxyl esterase are concurrently over expressed. A preventing, treating or recurrence suppression method of glioma includes a step of injecting the mesenchymal stem cell to all mammals except for human. A manufacturing method of human amniotic fluid-derived mesenchymal stem cell(AF-MSC) comprises the following steps: establishing positive number of primary cell cultivation in a minimum essential medium in which fetal bovine serum(ES-FBS) having 15% of embryonic stem cell, 2mM L-glutamine and 1% of penicillin streptomycin are contained, and 18% Chang culture medium B and 2% Chang culture medium C are supplemented; collecting cells by trypsinization for selective cultivation of AF-MSC; plating in DMEM low dextrose broth supplemented with 10% of FBS, 1% of penicillin streptomycin, 2mM L-glutamine and 4ng/mL basic fibroblast growth factor (bFGF: R&D); and obtaining morphologic interspecies population of the AF-MSC after second round of the subculture.

Abstract translation: 目的：提供内皮抑素和羧基酯酶同时过表达的间充质干细胞，通过使用胶质瘤预防，治疗或复发抑制方法，大幅度预防癌变和复发。 构成：在间充质干细胞中，内皮抑素和羧基酯酶同时过度表达。 胶质瘤的预防，治疗或复发抑制方法包括将间充质干细胞注射到除人之外的所有哺乳动物的步骤。 人类羊水间充质干细胞（AF-MSC）的制备方法包括以下步骤：在具有15％的胚胎干细胞的胎牛血清（ES-FBS）的最小必需培养基中建立阳性数量的原代细胞培养 细胞，2mM L-谷氨酰胺和1％青霉素链霉素，并补充18％Chang培养基B和2％Chang培养基C; 通过胰蛋白酶消化来收集细胞以选择性培养AF-MSC; 在补充有10％FBS，1％青霉素链霉素，2mM L-谷氨酰胺和4ng / mL碱性成纤维细胞生长因子（bFGF：R＆D）的DMEM低葡萄糖肉汤中电镀; 并在第二轮传代培养后获得AF-MSC的形态种间群体。