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公开(公告)号：KR101232850B1
公开(公告)日：2013-02-22
申请号：KR1020100115395
申请日：2010-11-19
Applicant: 서울대학교산학협력단 , 주식회사 옵티팜
Abstract: 본 발명은 서열번호 제1서열 내지 제5서열로 구성된 군으로부터 선택되는 뉴클레오타이드 서열에 특이적으로 결합하는 결합제를 포함하는 생식관의 발생 또는 분화 분석용 키트 및 이를 이용한 스크리닝 방법에 관한 것이다. 본 발명자들은 닭의 난관에 합성 에스트로겐, 바람직하게는 디에틸스틸베스트롤(diethylstilbestrol, DES)을 처리하여 실시한 전사체 프로파일(transcriptional profiling) 분석을 통해 생식관(바람직하게는, 난관) 발생 또는 분화-특이적 신규한 바이오마커인
CCRN4L (carbon catabolite repression 4-like),
NTM (neurotrimin),
HAS2 (hyaluronan synthase 2),
NELF (nasal embryonic LHRH factor) 및
FAM26F (family with sequence similarity 26, member F) 유전자를 동정하였다. 따라서, 본 발명의 마커 및 방법은 닭의 난관 발생에 필요한 유전자 분화 및 발생을 동정하기 위한 난관의 유전적 조절에 대한 새로운 시각을 제공한다.-
Patent Agency Ranking
公开(公告)号：KR1020120054151A
公开(公告)日：2012-05-30
申请号：KR1020100115395
申请日：2010-11-19
Applicant: 서울대학교산학협력단 , 주식회사 옵티팜
CPC classification number: G01N33/5302 , C12Q1/6837 , C12Q1/6844 , G01N33/74
Abstract: PURPOSE: A kit for analyzing development or differentiation of gonaduct is provided to identify gene differentiation and development. CONSTITUTION: A kit for analyzing development or differentiation of gonaduct contains a binder which specifically binds to a nucleotide sequence selected from sequence numbers 1-5. The expression of the nucleotide sequence is increases in the gonaduct by a material with estrogen activity. The material is synthetic estrogen or biogenic estrogen. The synthetic estrogen is diethyl stilbestrol. The binder is a probe, primer, antibody, or aptamer.
Abstract translation: 目的：提供分析发育或分化导管的试剂盒，以鉴别基因分化和发育。 构成：用于分析导管的发育或分化的试剂盒含有特异性结合选自序列号1-5的核苷酸序列的粘合剂。 核苷酸序列的表达是雌激素活性物质在导管中增加。 该物质是合成雌激素或生物雌激素。 合成雌激素是二苯乙烯雌酚。 粘合剂是探针，引物，抗体或适体。
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公开(公告)号：KR1020120057707A
公开(公告)日：2012-06-07
申请号：KR1020100080213
申请日：2010-08-19
Applicant: 서울대학교산학협력단
IPC: G01N33/574 , C12Q1/68 , C12N15/12 , G01N33/15
CPC classification number: G01N33/57449 , C12Q1/6886 , C12Q2600/158 , G01N33/15
Abstract: PURPOSE: A biomarker for diagnosing ovarian cancer and a cancer diagnosis kit are provided to simply and cheaply diagnose human ovarian cancer. CONSTITUTION: A kit for diagnosing cancer contains a binder which specifically binds to a nucleotide sequence encoding CLDN10(claudin 10) or MMP3(matix metalloproteinase 3) or a binder which is specific to CLDN10 or MMP3. The binder includes a probe, primer, antibody or aptamer. A pharmaceutical composition for preventing or treating cancer contains a material which suppresses CLDN10 or MMP3 expression as an active ingredient.
Abstract translation: 目的：诊断卵巢癌和癌症诊断试剂盒的生物标志物被简单且廉价地诊断人卵巢癌。 构成：用于诊断癌症的试剂盒含有特异性结合编码CLDN10（克隆蛋白10）或MMP3（matix金属蛋白酶3）的核苷酸序列或对CLDN10或MMP3特异的粘合剂的粘合剂。 粘合剂包括探针，引物，抗体或适体。 用于预防或治疗癌症的药物组合物含有抑制CLDN10或MMP3表达作为活性成分的材料。
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公开(公告)号：KR1020120054119A
公开(公告)日：2012-05-30
申请号：KR1020100115328
申请日：2010-11-19
Applicant: 서울대학교산학협력단 , 주식회사 옵티팜
IPC: A01K67/027 , C12N15/867 , C12N15/65
CPC classification number: A01K67/027 , C12N15/65 , C12N15/867
Abstract: PURPOSE: The production of transgenic aves using sequences for germ cell-specific gene expression is provided to analyze the differentiation or development of the priordial germ cells of aves into germ cells. CONSTITUTION: A method for producing transgenic aves includes the following: the priordial germ cells of aves are transformed based on a genetic vector containing a DAZL promoter as a germ cell-specific promoter, a NANOG promoter as a stem cell-specific promoter, and nucleotide sequence which encodes protein of revelation purpose; the transformed priordial germ cells of the aves are injected into accept media; and the transformed product of the aves is obtained. The protein is operatively bonded to the promoters.
Abstract translation: 目的：提供使用生殖细胞特异性基因表达序列的转基因抗生素的生产，以分析生物细胞中二次生殖细胞的分化或发育。 构成：用于生产转基因鸟类的方法包括以下：基于含有作为生殖细胞特异性启动子的DAZL启动子，作为干细胞特异性启动子的NANOG启动子和核苷酸的核苷酸的遗传载体转化aves的无性繁殖细胞 编码启示目的蛋白的序列; 将转化的雄性生殖细胞注入接受培养基; 并获得aves的转化产物。 蛋白质可操作地结合到启动子上。
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公开(公告)号：KR1020110052390A
公开(公告)日：2011-05-18
申请号：KR1020090109390
申请日：2009-11-12
Applicant: 서울대학교산학협력단
IPC: C12N15/63 , C12N15/12 , C12N5/0735 , A01K67/027
Abstract: PURPOSE: A recombinant expression vector and a method for screening a material which promotes germ cell development are provided to study of animal germ cell development. CONSTITUTION: A gene expression regulatory sequence contains a DAZL(deleted in AZoospermia-like) promoter containing a nucleotide sequence of sequence number 1 or and 3'UTR(3'untranslated region) of sequence number 2. A germ cell-specific recombinant vector contains DAZL promoter and expression target-coding nucleotide sequence which is operatively linked to the promoter. The germ cells are primordial germ cells or gonocyte or prespermatogonia. A method for screening a material which promotes germ cell development comprises: a step of transforming an animal cell by a vector containing DAZL-encoding nucleotide sequence; a step of treating test material to the animal cells; and a step of analyzing DAZL gene expression in the animal cells.
Abstract translation: 目的：提供重组表达载体和筛选促进生殖细胞发育的物质的方法，用于研究动物生殖细胞发育。 构成：基因表达调控序列含有含有序列号1的核苷酸序列和序列号2的3'UTR（3'非翻译区）的核苷酸序列的DAZL（缺失精子样）启动子。生殖细胞特异性重组载体含有 DAZL启动子和与启动子有效连接的表达靶标编码核苷酸序列。 生殖细胞是原始生殖细胞或者是gonocyte或prepermatogonia。 用于筛选促进生殖细胞发育的材料的方法包括：通过含有编码DAZL的核苷酸序列的载体转化动物细胞的步骤; 向动物细胞处理试验材料的步骤; 以及在动物细胞中分析DAZL基因表达的步骤。
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公开(公告)号：KR101423971B1
公开(公告)日：2014-08-04
申请号：KR1020100115328
申请日：2010-11-19
Applicant: 서울대학교산학협력단 , 주식회사 옵티팜
IPC: A01K67/027 , C12N15/867 , C12N15/65
Abstract: 본 발명은 NANOG 프로모터 또는 DAZL 프로모터-목적 단백질 인코딩 서열의 컨스트럭트를 이용하여 형질전환 조류를 최초로 구축한 것이다. 본 발명은 조류 원시생식세포로부터 생식세포로의 분화 또는 발달 연구에 매우 유용한 툴을 제공한다. 본 발명의 형질전환 조류를 이용하면, 생식세포 발달과정 동안 전능성의 조절 기전을 규명할 수 있고, 또한 생식세포 특정화(germ cell specification)가 조류서 어느 시기에 이루어지는지 규명할 수 있다.
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