펩타이드 라이브러리를 이용한 세린/트레오닌 인산화효소의 기질특이성 분석 방법
    2.
    发明公开
    펩타이드 라이브러리를 이용한 세린/트레오닌 인산화효소의 기질특이성 분석 방법 有权
    使用OBOC图书馆鉴定蛋白激酶底物特异性的方法

    公开(公告)号:KR1020100051758A

    公开(公告)日:2010-05-18

    申请号:KR1020080110718

    申请日:2008-11-08

    CPC classification number: C12Q1/485 G01N2333/9121

    Abstract: PURPOSE: A method for identifying protein kinase substrate specificity using a peptide library is provided to rapidly determining the substrate specificity of a protein phosphatase, and to examine the role of the protein phosphatase. CONSTITUTION: A method for identifying protein kinase substrate specificity comprises the following steps: forming a peptide library with a constant amino acid sequence on the surface of a solid support resin; forming a hybrid with randomized peptide libraries by repeating the previous step; reacting the peptide libraries with a phosphatase for forming the peptide library with phosphorylated threonine or serine; reacting an antibody combined with a biotin with the phosphorylated peptide library; reacting the obtained product with neutravidin or streptavidin including phosphatase or peroxidase; detecting the change of properties by the reaction of the phosphatase or the peroxidase for selecting the solid support resin with transformed peptide libraries; and an analyzing the amino acid sequence of the peptide after separating the peptide from the resin.

    Abstract translation: 目的:提供使用肽文库鉴定蛋白激酶底物特异性的方法,以快速测定蛋白磷酸酶的底物特异性,并检查蛋白磷酸酶的作用。 构成:鉴定蛋白激酶底物特异性的方法包括以下步骤:在固体支持树脂的表面上形成具有恒定氨基酸序列的肽文库; 通过重复前述步骤与随机肽文库形成杂交体; 使肽文库与磷酸酶反应,用磷酸化苏氨酸或丝氨酸形成肽文库; 将与生物素结合的抗体与磷酸化肽文库反应; 使所得产物与中性链亲和素或链霉亲和素(包括磷酸酶或过氧化物酶)反应; 通过磷酸酶或过氧化物酶的反应检测特性的变化,用于用转化的肽文库选择固体支持树脂; 并分析肽与树脂分离后的氨基酸序列。

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