Abstract:
본 발명은 신규 리조비움 sp. GIN611( Rhizobium sp.GIN611)KCTC11708BP또는 그의 세포 추출물, 당 분해 활성을 보이는 신규 산화환원 효소, 이를 암호화하는 유전자 및 재조합 벡터 단백질, 또는 재조합 단백질을 암호화하는 발현벡터를 포함하는 재조합 균주 등을 제공하고, 이들을 생촉매로 사용하여 천연물을 당 분해하는 방법을 제공한다. 또한, 이들을 이용하여 다양한 천연물의 비당체를 생산하는 방법을 제공한다. 본 발명의 신규 미생물에서 분리한 신규한 효소는 글루코시데이즈(glucosidase)군에 속하지 않고 산화환원효소 군에 속하는 효소로서 천연물의 당 분해 활성을 갖는다. 이 신규한 산화환원효소는 천연물 배당체에서 당을 산화시킴으로서 다양한 비당체를 생산할 수 있게 한다.
Abstract:
본 명세서는 테아시넨신 A, 이의 이성질체, 이의 약학적으로 허용가능한 염, 이의 수화물 또는 이의 용매화물을 유효성분으로 포함하는 조성물에 관한 것이다. 상기 조성물은 우수한 안정성을 가지며, 테아시넨신 A, 이의 이성질체, 이의 약학적으로 허용가능한 염, 이의 수화물 또는 이의 용매화물을 유효성분으로 포함함으로써 피부 주름 또는 탄력 개선효능, 미백효능을 제공한다.
Abstract:
PURPOSE: A method for preparing ginsenoside using a novel oxidoreductase or novel Rhizobium sp. GIN611 is provided to ensure produce various glycons. CONSTITUTION: A novel Rhizobium sp. GIN611(KCTC 11708P) is isolated from soil. A method for glycolysis of a natural product comprises a step of decomposing glucose of ginsenoside. A substrate is ginsenoside compound K and the product is ginsenoside PPD(S). An oxidoreductase contains an amino acid sequence of sequence number 3. A DNA encoding the oxidoreductase contains a sequence of sequence number 2. The oxidoreductase is derived from Agrobacterium sp., Sphingobacterium sp. or Stenotrophomonase sp.
Abstract:
PURPOSE: A Staphylococcus hominis MBBL 2-9 strain is provided to ensure antibacterial activity to bacteria and to prevent gram positive/antibiotics resistant pathogen. CONSTITUTION: An antibacterial peptide hominicin which is isolated from Staphylococcus hominis MBBL 2-9 has an amino acid of sequence number 1. The antibacterial peptide is themal resistant, acid resistant, basic resistant, and non-cytotoxicity. The antibacterial peptide contains DmIle(N1, N1-dimethyl isoleucine), Dhb(dehydrobutyrine), and Dmp(N2, N2-dimethyl-1,2-propanediamine). A medicinal composition for preventing gram positive pathogen or antibiotics resistant pathogen contains the antibacterial peptide as an active ingredient.
Abstract:
PURPOSE: A novel soil microorganism and oxidoreductase isolated from the microorganism are provided to ensure saccharide decomposition of natural products and to obtain various flavonoid aglycons. CONSTITUTION: A method for preparing flavonoid aglycons comprises a step of using Rhizobium sp. GIN611(KCTC11708BP) or cell extract thereof. The strain is isolated from soil. The flavonoid aglycon is prepared using Rhizobium sp. GIN611-derived oxidoreductase as a biocatalyst. The flavonoid aglycons are prepared by decomposing saccharides from flavonoid glycoside.
Abstract:
PURPOSE: A method for identifying protein kinase substrate specificity using a peptide library is provided to rapidly determining the substrate specificity of a protein phosphatase, and to examine the role of the protein phosphatase. CONSTITUTION: A method for identifying protein kinase substrate specificity comprises the following steps: forming a peptide library with a constant amino acid sequence on the surface of a solid support resin; forming a hybrid with randomized peptide libraries by repeating the previous step; reacting the peptide libraries with a phosphatase for forming the peptide library with phosphorylated threonine or serine; reacting an antibody combined with a biotin with the phosphorylated peptide library; reacting the obtained product with neutravidin or streptavidin including phosphatase or peroxidase; detecting the change of properties by the reaction of the phosphatase or the peroxidase for selecting the solid support resin with transformed peptide libraries; and an analyzing the amino acid sequence of the peptide after separating the peptide from the resin.