Abstract:
코드화된 고분자 미세입자 코어; 및 상기 미세입자 코어를 둘러싼 실리카 쉘을 포함하는 코드화된 고분자 미세입자 및 이를 이용한 다중 바이오 분석방법이 제공된다. 또한 광경화성 물질, 및 상기 광경화성 물질과 중합가능한 작용기와 알콕시실릴기를 함께 구비한 링커의 혼함물을 제공하는 단계; 패턴화된 에너지를 인가하여 상기 혼합물을 경화하고, 코드화함으로써 코드화된 고분자 미세입자 코어를 얻는 단계; 및 상기 코드화된 고분자 미세입자 코어에 실리카 전구체를 처리하여 상기 코드화된 고분자 미세입자 코어 위에 실리카 쉘을 형성하는 단계를 포함하는 코드화된 고분자 미세입자의 제조방법이 제공된다.
Abstract:
PURPOSE: A method for identifying protein kinase substrate specificity using a peptide library is provided to rapidly determining the substrate specificity of a protein phosphatase, and to examine the role of the protein phosphatase. CONSTITUTION: A method for identifying protein kinase substrate specificity comprises the following steps: forming a peptide library with a constant amino acid sequence on the surface of a solid support resin; forming a hybrid with randomized peptide libraries by repeating the previous step; reacting the peptide libraries with a phosphatase for forming the peptide library with phosphorylated threonine or serine; reacting an antibody combined with a biotin with the phosphorylated peptide library; reacting the obtained product with neutravidin or streptavidin including phosphatase or peroxidase; detecting the change of properties by the reaction of the phosphatase or the peroxidase for selecting the solid support resin with transformed peptide libraries; and an analyzing the amino acid sequence of the peptide after separating the peptide from the resin.
Abstract:
Provided are an encoded polymer microparticles comprising an encoded polymer microparticle core and a silica shell encompassing the microparticle core; and a multiplexed bioanalysis method using the same. In addition, provided is a method for preparing an encoded polymer microparticles, comprising the steps of: providing a mixture of a radiation curable material, and a linker comprising a functional group capable of being polymerized with the radiation curable material, and an alkoxysilyl group; obtaining an encoded polymer microparticle core by applying a patterned energy to cure the mixture and encoding the same; and forming a silica shell on the encoded polymer microparticle core by treating the encoded polymer microparticle core with a silica precursor.