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公开(公告)号：KR101849409B1
公开(公告)日：2018-06-01
申请号：KR1020110148029
申请日：2011-12-31
Applicant: 서울대학교산학협력단
CPC classification number: C12Q1/6886 , C12Q2600/136 , C12Q2600/154 , G01N33/5011
Abstract: 본발명은폰틴의메틸화를이용한항암제스크리닝방법에관한것으로서,폰틴(pontin)의메틸화을억제하는것이암 발생및 전이를억제하는항암물질인것으로판단하는항암제를스크리닝하는방법을제공한다. 보다상세하게는세포를배양하는단계; 상기세포에잠재적인물질을접촉시키는단계; 상기세포내 폰틴의메틸화를대조구(상기세포에상기잠재적인물질을접촉시키지않은시험구) 대비감소하는것을확인하는단계; 및상기폰틴의메틸화를감소시키는상기잠재성물질을항암제로선택하는단계를포함하는것을특징으로하는항암제스크리닝방법을제공한다.
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公开(公告)号：KR1020100127403A
公开(公告)日：2010-12-06
申请号：KR1020090045833
申请日：2009-05-26
Applicant: 서울대학교산학협력단
Abstract: PURPOSE: A method for identifying a material which negatively controls hypoxic target gene and a composition for treating cancer using the identified test material are provided. CONSTITUTION: A composition for treating cancer contains a material which negatively controls hypoxic target gene. The material is a methyltansferase which methylates intracellular leptin. The methyltransferase is G9a. A method for identifying the material comprises: a step of treating a test material to cells under the hypoxic environment; a step of detecting methylation of intracellular leptin; and a step of determining the material which negatively controls hypoxic target gene which methylates reptin.
Abstract translation: 目的：提供一种用于鉴定使用所鉴定的测试材料负面控制缺氧靶基因的材料和用于治疗癌症的组合物的方法。 构成：用于治疗癌症的组合物含有负调控缺氧靶基因的物质。 该物质是甲基化酶细胞内瘦素的甲基化酶。 甲基转移酶是G9a。 用于鉴定材料的方法包括：将测试材料处理在缺氧环境下的细胞的步骤; 检测细胞内瘦素甲基化的步骤; 以及确定负调控甲酸化赖氨酸的缺氧靶基因的材料的步骤。
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公开(公告)号：KR1020130078865A
公开(公告)日：2013-07-10
申请号：KR1020110148029
申请日：2011-12-31
Applicant: 서울대학교산학협력단
CPC classification number: C12Q1/6886 , C12Q2600/136 , C12Q2600/154 , G01N33/5011
Abstract: PURPOSE: A method for screening anticancer agents using methylation level of pontin is provided to screen anticancer agents and to develop novel anticancer drugs through the effect on cancer cell proliferation and growth by controlling the bond of a methyl group to the pontin protein. CONSTITUTION: A method for screening anticancer agents comprises the steps of culturing cells; contacting the cells with potential materials; confirming reduction of methylation of intracellular pontin compared with a control group (uncontacted group); and selecting a potential material which reduces methylation of pontin as an anticancer agent. The cell is an animal cell.
Abstract translation: 目的：提供使用甲基化水平的聚合酶链反应筛选抗癌药物的方法，通过控制甲基与蛋白连接蛋白的结合，通过对癌细胞增殖和生长的影响来筛选抗癌剂和开发新的抗癌药物。 构成：筛选抗癌剂的方法包括培养细胞的步骤; 使细胞与潜在的材料接触; 确认与对照组（未接触组）相比，细胞内pontin的甲基化减少; 并选择减少作为抗癌剂的聚乙烯甲基化的潜在材料。 细胞是动物细胞。
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公开(公告)号：KR101142588B1
公开(公告)日：2012-05-10
申请号：KR1020090045833
申请日：2009-05-26
Applicant: 서울대학교산학협력단
Abstract: PURPOSE: A method for identifying a material which negatively controls hypoxic target gene and a composition for treating cancer using the identified test material are provided. CONSTITUTION: A composition for treating cancer contains a material which negatively controls hypoxic target gene. The material is a methyltansferase which methylates intracellular leptin. The methyltransferase is G9a. A method for identifying the material comprises: a step of treating a test material to cells under the hypoxic environment; a step of detecting methylation of intracellular leptin; and a step of determining the material which negatively controls hypoxic target gene which methylates reptin.
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