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公开(公告)号：KR100980444B1
公开(公告)日：2010-09-07
申请号：KR1020080018561
申请日：2008-02-28
Applicant: 성균관대학교산학협력단
Abstract: 본 발명은 활성을 유지한 목표 단백질의 발현을 측정할 수 있는 재조합 유전자 발현 벡터 및 이의 이용에 관한 것으로, 구체적으로 재조합 형질전환체를 이용한 목표 단백질의 수득 및 발현 측정 방법에 있어서 목표 단백질 유전자의 하류에 프레임쉬프팅 서열과 검출표지를 가진 파트너 단백질 유전자를 결합시킨 재조합 발현 벡터를 제공하고 이를 이용하여 활성을 유지한 목표 단백질을 수득하면서 이의 발현을 측정하는 방법에 관한 것이다.
재조합 발현 벡터, 활성유지, 파트너 단백질, 발현, 정량적 측정-
公开(公告)号：KR1020100119018A
公开(公告)日：2010-11-09
申请号：KR1020090037915
申请日：2009-04-30
Applicant: 삼성전자주식회사 , 성균관대학교산학협력단
CPC classification number: C12P7/10 , C08H8/00 , C12P2201/00 , C13K1/02 , Y02E50/16 , Y02E50/343 , Y02P30/20 , C08B1/08 , C10G3/00 , C12P7/04 , C12R1/865
Abstract: PURPOSE: A pretreatment method of a lignocellulose-based biomass is provided to obtain cellulose with high purity and hemicelluloses with the high yield, by extracting lignin first. CONSTITUTION: A pretreatment method of a lignocellulose-based biomass comprises the following steps: adding a solvent dissolving lignin to the lignocellulose-based biomass containing the lignin, hemicelluloses, and cellulose, to extract the lignin from the biomass(S1,S2); and adding an ionic solution to the biomass without the lignin, to extract the hemicelluloses and the cellulose(S3). The solvent dissolving the lignin is an alkaline solvent.
Abstract translation: 目的：提供木质纤维素基生物质的预处理方法，首先通过提取木质素，以高产率获得高纯度的纤维素和半纤维素。 构成：木质纤维素基生物质的预处理方法包括以下步骤：向含木质素，半纤维素和纤维素的木质纤维素基生物质中加入溶解木质素的溶剂，从生物质提取木质素（S1，S2）; 并将离子溶液加入到没有木质素的生物质中，以提取半纤维素和纤维素（S3）。 溶解木质素的溶剂是碱性溶剂。
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公开(公告)号：KR1020090093186A
公开(公告)日：2009-09-02
申请号：KR1020080018561
申请日：2008-02-28
Applicant: 성균관대학교산학협력단
Abstract: A recombinant gene expression vector which is able to measure the expression of target protein is provided to measure the amount of protein expression and predict the amount of expression and activation of target protein. A recombinant expression vector comprises a frame shifting sequence selected among a frame shifting sequence having the sequence number 1; a frame shifting sequence having a sequence number 2; a frame shifting sequence having a sequence number 3 and a frame shifting sequence having a sequence number 4 and partner protein gene having detection marker.
Abstract translation: 提供能够测量靶蛋白表达的重组基因表达载体，以测量蛋白质表达量并预测靶蛋白的表达和活化量。 重组表达载体包括从具有序列号1的帧移位序列中选择的帧移位序列; 具有序列号2的帧移位序列; 具有序列号3的帧移位序列和具有序列号4的帧移位序列和具有检测标记的伴侣蛋白基因。
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