公开(公告)号：KR1020030067871A

公开(公告)日：2003-08-19

申请号：KR1020020007374

申请日：2002-02-08

Applicant: 안국바이오진단(주)

Inventor： 송희경 ,  김정미 ,  조장은 ,  박신이 ,  윤성욱 ,  한상엽

IPC: C12Q1/68

Abstract: PURPOSE: A lineage specific DNA microarray and a method for monitoring cell differentiation using the sam are provided, thereby easily and simply monitoring cell differentiation. CONSTITUTION: A lineage specific DNA microarray for monitoring cell differentiation comprises a marker gene inducing the cell differentiation or a probe capable of detecting the tissue specifically expressed marker gene and a substrate fixing the probe, wherein the probe is cDNA or oligonucleotide having 10 to 1000 bases to the marker gene; the marker gene is one or more gene selected from undifferentiated marker gene, endoderm marker gene, mesoderm marker gene, ectoderm marker gene and tissue marker gene derived from the germinal layer; the undifferentiated marker gene is OCT-4(octamer binding protein-4) or alkaline phosphorase; and the substrate is selected from silicone wafer, glass, polycarbonate, membrane and polymer film.

Abstract translation: 目的：提供谱系特异性DNA微阵列和使用sam监测细胞分化的方法，从而容易且简单地监测细胞分化。 构成：用于监测细胞分化的谱系特异性DNA微阵列包括诱导细胞分化的标记基因或能够检测组织特异性标记基因的探针和固定探针的底物，其中所述探针是具有10至1000个碱基的cDNA或寡核苷酸 标记基因; 标记基因是选自未分化标记基因，内胚层标记基因，中胚层标记基因，外胚层标记基因和源自生发层的组织标记基因的一种或多种基因; 未分化标记基因是OCT-4（八聚体结合蛋白-4）或碱性磷酸酶; 基板选自硅胶片，玻璃，聚碳酸酯，膜和聚合物膜。