公开(公告)号：KR100464259B1

公开(公告)日：2005-01-03

申请号：KR1020020039464

申请日：2002-07-08

Applicant: 안국바이오진단(주) ,  재단법인한국간연구재단

Inventor： 김범준 ,  조문제 ,  국윤호 ,  김정미 ,  박정민

IPC: C12Q1/68

Abstract: PURPOSE: An identification method of Streptomyces having rifampin resistance or sensitivity using rpoB gene, and a primer specific to Streptomyces having rifampin resistance or sensitivity are provided, thereby simplifying the method, decreasing the costs therefor and improving the accuracy thereof. CONSTITUTION: An identification method of Streptomyces having rifampin resistance or sensitivity is characterized by using the nucleotide sequence difference encoding an amino acid of a rpoB gene of Streptomyces sp. corresponding to the 352th amino acid in RNA polymerase subunit B of Streptomyces coelicolor, wherein the nucleotide sequence encoding the amino acid is 258-bp to 260-bp of nucleotide sequence from the 5-terminal of a 352-bp fragment obtained by amplifying the rpoB gene of Streptomyces sp. using a pair of primers having the nucleotide sequences of SEQ ID NO: 1 and SEQ ID NO: 2.

Abstract translation: 目的：提供使用rpoB基因的具有利福平抗性或灵敏度的链霉菌的鉴定方法，以及对具有利福平耐受性或灵敏度的链霉菌特异性的引物，从而简化了方法，降低了成本并提高了其准确度。 构成：具有利福平抗性或敏感性的链霉菌的鉴定方法的特征在于使用编码链霉菌属的rpoB基因的氨基酸的核苷酸序列差异。 其对应于天蓝色链霉菌的RNA聚合酶亚基B中的第352位氨基酸，其中编码该氨基酸的核苷酸序列是从扩增rpoB获得的352-bp片段的5'端的核苷酸序列的258-bp至260-bp 链霉菌属的基因。 使用具有SEQ ID NO：1和SEQ ID NO：2的核苷酸序列的一对引物。

公开(公告)号：KR100460747B1

公开(公告)日：2004-12-08

申请号：KR1020020054330

申请日：2002-09-09

Applicant: 안국바이오진단(주)

Inventor： 김정미 ,  이지영 ,  윤성욱

IPC: C12Q1/68

Abstract: PURPOSE: A probe for detecting human herpesvirus(HHV), a genotyping kit and a genotyping method for human herpesvirus using the same are provided, thereby rapidly, simply and accurately detecting HHV which consists of Herpes Simplex virus type 1(HSV-1), HSV-2 and human herpesvirus-8(HHV-8) and causes STD(sexual transmitted disease). CONSTITUTION: A probe for detecting human herpesvirus(HHV) is selected from oligonucleotides having the nucleotide sequences of SEQ ID NO: 1 to SEQ ID NO: 9 and complementary oligonucleotides thereof and complementarily binds to the nucleic acid of human herpesvirus. A DNA chip for detecting human herpesvirus(HHV) or genotyping of HHV contains at least one probes selected from oligonucleotides having the nucleotide sequences of SEQ ID NO: 1 to SEQ ID NO: 9 and complementary oligonucleotides thereof. A genotyping kit for human herpesvirus comprises (a) the DNA chip for detecting human herpesvirus, (b) a PCR primer and (c) a marker means for detecting amplified DNA hybridized with the DNA chip. A genotyping method for human herpesvirus comprises the steps of: (a) amplifying a sample DNA using the PCR primer; (b) hybridizing the amplified DNA on the DNA chip containing one probe or two or more of probe sets; and (c) detecting the product hybridized with the probe.

Abstract translation: 目的：提供一种用于检测人类疱疹病毒（HHV）的探针，基因分型试剂盒和使用其的人类疱疹病毒基因分型方法，从而快速，简单且准确地检测由单纯疱疹病毒1型（HSV-1）， HSV-2和人类疱疹病毒-8（HHV-8）并导致STD（性传播疾病）。 构成：用于检测人疱疹病毒（HHV）的探针选自具有SEQ ID NO：1至SEQ ID NO：9的核苷酸序列的寡核苷酸及其互补寡核苷酸，并与人类疱疹病毒的核酸互补结合。 用于检测人疱疹病毒（HHV）或HHV基因分型的DNA芯片含有至少一种选自具有SEQ ID NO：1至SEQ ID NO：9的核苷酸序列的寡核苷酸及其互补寡核苷酸的探针。 （a）用于检测人类疱疹病毒的DNA芯片，（b）PCR引物和（c）用于检测与DNA芯片杂交的扩增DNA的标记手段。 用于人疱疹病毒的基因分型方法包括以下步骤：（a）使用PCR引物扩增样品DNA; （b）在含有一个探针或两个或多个探针组的DNA芯片上杂交扩增的DNA; 和（c）检测与探针杂交的产物。

公开(公告)号：KR1020030067871A

公开(公告)日：2003-08-19

申请号：KR1020020007374

申请日：2002-02-08

Applicant: 안국바이오진단(주)

Inventor： 송희경 ,  김정미 ,  조장은 ,  박신이 ,  윤성욱 ,  한상엽

IPC: C12Q1/68

Abstract: PURPOSE: A lineage specific DNA microarray and a method for monitoring cell differentiation using the sam are provided, thereby easily and simply monitoring cell differentiation. CONSTITUTION: A lineage specific DNA microarray for monitoring cell differentiation comprises a marker gene inducing the cell differentiation or a probe capable of detecting the tissue specifically expressed marker gene and a substrate fixing the probe, wherein the probe is cDNA or oligonucleotide having 10 to 1000 bases to the marker gene; the marker gene is one or more gene selected from undifferentiated marker gene, endoderm marker gene, mesoderm marker gene, ectoderm marker gene and tissue marker gene derived from the germinal layer; the undifferentiated marker gene is OCT-4(octamer binding protein-4) or alkaline phosphorase; and the substrate is selected from silicone wafer, glass, polycarbonate, membrane and polymer film.

Abstract translation: 目的：提供谱系特异性DNA微阵列和使用sam监测细胞分化的方法，从而容易且简单地监测细胞分化。 构成：用于监测细胞分化的谱系特异性DNA微阵列包括诱导细胞分化的标记基因或能够检测组织特异性标记基因的探针和固定探针的底物，其中所述探针是具有10至1000个碱基的cDNA或寡核苷酸 标记基因; 标记基因是选自未分化标记基因，内胚层标记基因，中胚层标记基因，外胚层标记基因和源自生发层的组织标记基因的一种或多种基因; 未分化标记基因是OCT-4（八聚体结合蛋白-4）或碱性磷酸酶; 基板选自硅胶片，玻璃，聚碳酸酯，膜和聚合物膜。

公开(公告)号：KR1020030025787A

公开(公告)日：2003-03-29

申请号：KR1020020030285

申请日：2002-05-30

Applicant: 안국바이오진단(주)

Inventor： 조용구 ,  김현정 ,  김정미

IPC: G01N33/53

Abstract: PURPOSE: A quantitative method of protein using surface plasmon resonance(SPR) is provided, thereby specified disease and down syndrome can be diagnosed. CONSTITUTION: The quantitative method of a down syndrome-mark protein comprises the steps of: (a) reacting a self assembled monolayer compound with a surface plasmon layer to prepare the self assemble monolayer; (b) fixing an antibody of a target protein to the self assembled monolayer; (c) reacting a sample containing the target protein with the fixed antibody; (d) irradiating light to the surface plasmon layer to measure a change of refraction value; and (e) conversing the amount of the target protein using the change of refraction value and standardized amount of the target protein, wherein the compound of the step (a) has a function group being capable of the self assembled monolayer and regions recognizing positive ion; the function group is thiol, and the positive ion recognizing region is a crown ring; the light is 800 to 850 nm of light from light emitting diode(LED) and is detected by using photodiode array; the protein is alpha-fetoprotein, human chorionic gonadotropin or unconjugated estriol; and the antibody is monoclonal antibody or polyclonal antibody.

Abstract translation: 目的：提供使用表面等离子体共振（SPR）的蛋白质的定量方法，从而可以诊断出疾病和衰退综合征。 构成：下调综合征标记蛋白的定量方法包括以下步骤：（a）使自组装单层化合物与表面等离子体层反应，制备自组装单层; （b）将靶蛋白的抗体固定在自组装单层上; （c）使含有靶蛋白的样品与固定抗体反应; （d）向表面等离子体膜照射光以测量折射值的变化; （e）使用目标蛋白质的折射值和标准化量的变化对目标蛋白质的量进行转化，其中步骤（a）的化合物具有能够自组装单层的功能基团和​​识别阳离子的区域 ; 功能组为硫醇，正离子识别区为冠环; 光是发光二极管（LED）的800至850nm的光，并通过使用光电二极管阵列进行检测; 蛋白质是甲胎蛋白，人绒毛膜促性腺激素或非共轭雌三醇; 抗体是单克隆抗体或多克隆抗体。

公开(公告)号：KR1020030015124A

公开(公告)日：2003-02-20

申请号：KR1020020036731

申请日：2002-06-28

Applicant: 안국바이오진단(주) ,  김범준 ,  국윤호

Inventor： 김범준 ,  김정미 ,  국윤호 ,  이근화 ,  천종식 ,  배경숙

IPC: C12Q1/68

Abstract: PURPOSE: A primer set specific to genus Streptomyces, rpoB gene fractions, and an identification method of genus Streptomyces by using the same are provided, thereby identifying the genus Streptomyces easily and cheaply. CONSTITUTION: The primer set specific to genus Streptomyces is selected from the group consisting of primers having the nucleotide sequences of SEQ ID NOs: 1 and 2, specifically amplifying RNA polymerase B(rpoB) subunit gene. The polynucleotide is selected from the nucleotide sequences of SEQ ID NOs: 3 to 164 and comprises 306 bp of the rpoB gene fraction of the genus Streptomyces. The identification method of genus Streptomyces comprises the steps of: (i) amplifying the rpoB gene fraction of a strain using the primer set specific to the genus Streptomyces; (ii) sequencing the amplified ropB gene fractions; and (iii) comparing the nucleotide sequences analyzed in the step (ii) and the rpoB gene sequence.

Abstract translation: 目的：提供特异性链霉菌属的引物组，rpoB基因级分，以及通过使用该引物对链霉菌属的鉴定方法，从而易于且便宜地鉴定链霉菌属。 构成：特异于链霉菌属的引物组选自具有SEQ ID NO：1和2的核苷酸序列的引物组，特异性扩增RNA聚合酶B（rpoB）亚基基因。 多核苷酸选自SEQ ID NO：3至164的核苷酸序列，并且包含306bp的链霉菌属的rpoB基因级分。 链霉菌属的鉴定方法包括以下步骤：（i）使用特异于链霉菌属的引物组扩增菌株的rpoB基因级分; （ii）测序扩增的ropB基因级分; 和（iii）比较步骤（ii）中分析的核苷酸序列和rpoB基因序列。

公开(公告)号：KR100532665B1

公开(公告)日：2005-12-02

申请号：KR1020020074644

申请日：2002-11-28

Applicant: 안국바이오진단(주)

Inventor： 김정미 ,  김지호 ,  윤성욱

IPC: C12Q1/68

Abstract: 본 발명은 나이세리아속 균, 바람직하게는 임균(
Neisseria gonorrhoeae ) 또는 수막염균 (
Neisseria meningitidis )의 핵산과 상보적으로 결합하는 프로브, 이를 포함하는 DNA 칩을 사용하여 임균 및/또는 수막염균의 감염 및 유전형을 진단하는 임균 및/또는 수막염균의 유전형 분석키트, 및 상기 분석키트를 사용하여 임균 및/또는 수막염균의 감염여부를 탐지하는 방법에 관한 것이다. 본 발명에 따른 유전형 분석방법은 종래에 도말검사나 배양 검사에 비해 더 빠르고, 정확하게 실험 결과를 도출해 낼 수 있는 장점을 가지고 있다. 뿐만 아니라, 한번의 실험으로 임균 및/또는 수막염균의 혈청형까지 구분해 낼 수 있다.

公开(公告)号：KR100517275B1

公开(公告)日：2005-09-27

申请号：KR1020030013464

申请日：2003-03-04

Applicant: 안국바이오진단(주)

Inventor： 윤성욱 ,  김정미

IPC: C12Q1/68

Abstract: 본 발명은 인유두종 바이러스의 DNA 또는 RNA에 결합하는 프로브, 이를 포함하는 DNA 칩을 사용하여 인유두종 바이러스의 감염 및 유전형을 진단하는 인유두종 바이러스의 감염여부를 진단할 수 있는 DNA 칩 및 유전형 분석키트에 관한 것이다.

公开(公告)号：KR1020040024704A

公开(公告)日：2004-03-22

申请号：KR1020020056131

申请日：2002-09-16

Applicant: 안국바이오진단(주)

Inventor： 조용구 ,  김현정 ,  김정미

IPC: G01N21/00

Abstract: PURPOSE: An integrated flow cell for a surface plasmon resonance sensor is provided to detect a biological defect and to rapidly perform a somatological analysis by forming various micro fluidic elements in plural layers. CONSTITUTION: A flow cell has a layer structure including a fluid inlet layer(1), a sample injection layer(2), a reaction space layer(3), a first fluid distributing layer(4), a second fluid distributing layer(5), and a bonding layer(6). The layers included in the layer structure are made from a polymeric substrate. A fluid element, such as a channel, is formed at a lower surface of each layer of the flow cell. Each layer of the layer structure is connected to a channel or an end of a chamber through a port. A pair of fluid injection ports is formed in the fluid injection layer(1) so as to inject fluid into the flow cell.

Abstract translation: 目的：提供一种用于表面等离子体共振传感器的集成流通池，用于检测生物缺陷，并通过在多层中形成各种微流体元素来快速进行体细胞学分析。 构成：流动池具有层结构，其包括流体入口层（1），样品注入层（2），反应空间层（3），第一流体分配层（4），第二流体分配层（5） ）和结合层（6）。 层结构中包含的层由聚合物基材制成。 诸如通道的流体元件形成在流动池的每个层的下表面处。 层的每个层通过端口连接到腔室的通道或端部。 在流体注入层（1）中形成一对流体注入口，以将流体注入到流动池中。

公开(公告)号：KR1020040047170A

公开(公告)日：2004-06-05

申请号：KR1020020075284

申请日：2002-11-29

Applicant: 안국바이오진단(주)

Inventor： 김정미 ,  이지영 ,  한상엽

IPC: C12Q1/68

Abstract: PURPOSE: A probe for detecting Treponema pallidum, and a kit and method for detecting Treponema pallidum using the same are provided, thereby specifically and sensitively detecting Treponema pallidum and Treponema tpr(Treponema pallidum repeat) subfamily at one time. CONSTITUTION: The probe for detecting Treponema pallidum is selected from oligonucleotides having the nucleotide sequences set forth in SEQ ID NO: 1 to SEQ ID NO: 5, and complementary oligonucleotides thereof, and complementarily binds with the nucleic acid of Treponema pallidum. A DNA chip for detecting Treponema pallidum comprises the probe for detecting Treponema pallidum, consisting of oligonucleotides containing a portion of the tpr gene of Treponema pallidum. The kit for detecting Treponema pallidum comprises (a) the DNA chip for detecting Treponema pallidum; (b) a primer for PCR amplification of the sample DNA of Treponema pallidum; and (c) a labeling marker for detecting DNA hybridized with the DNA chip, wherein the labeling marker is selected from Cy5, Cy3, biotin-binding material, EDANS (5-(2'-aminoethyl)amino-1-naphthalene sulfuric acid), tetramethyl-rhodamine(TMR), tetramethyl-rhodamine isocyanate(TMRITC), x-rhodamine and texas red. The method for detecting Treponema pallidum comprises the steps of: (a) PCR amplifying the sample DNA of Treponema pallidum using the primer; (b) hybridizing the amplified DNA with the DNA chip; and detecting products hybridized with the probe.

Abstract translation: 目的：提供用于检测梅毒螺旋体的探针，以及使用该探针来检测苍白密螺旋体的试剂盒和方法，从而特异性地并且一次灵敏地检测苍白密螺旋体梅毒螺旋体（Treponema pallidum repeat）亚科。 构成：用于检测苍白密螺旋体的探针选自具有SEQ ID NO：1至SEQ ID NO：5所示核苷酸序列的寡核苷酸及其互补寡核苷酸，并与苍白密螺旋体的核酸互补结合。 用于检测苍白密螺旋体的DNA芯片包括由梅毒螺旋体的一部分tpr基因的寡核苷酸组成的用于检测苍白密螺旋体的探针。 用于检测苍白密螺旋体的试剂盒包括（a）用于检测苍白密螺旋体的DNA芯片; （b）用于PCR扩增苍白密螺旋体样品DNA的引物; （c）用于检测与DNA芯片杂交的DNA的标记标记，其中所述标记标记选自Cy5，Cy3，生物素结合材料，EDANS（5-（2'-氨基乙基）氨基-1-萘硫酸） ，四甲基罗丹明（TMR），四甲基 - 若丹明异氰酸酯（TMRITC），x-罗丹明和德克萨斯红。 用于检测苍白密螺旋体的方法包括以下步骤：（a）使用引物PCR扩增苍白密螺旋体样品DNA; （b）将扩增的DNA与DNA芯片杂交; 并检测与探针杂交的产物。

公开(公告)号：KR1020040022800A

公开(公告)日：2004-03-18

申请号：KR1020020054330

申请日：2002-09-09

Applicant: 안국바이오진단(주)

Inventor： 김정미 ,  이지영 ,  윤성욱

IPC: C12Q1/68

Abstract: PURPOSE: A probe for detecting human herpesvirus(HHV), a genotyping kit and a genotyping method for human herpesvirus using the same are provided, thereby rapidly, simply and accurately detecting HHV which consists of Herpes Simplex virus type 1(HSV-1), HSV-2 and human herpesvirus-8(HHV-8) and causes STD(sexual transmitted disease). CONSTITUTION: A probe for detecting human herpesvirus(HHV) is selected from oligonucleotides having the nucleotide sequences of SEQ ID NO: 1 to SEQ ID NO: 9 and complementary oligonucleotides thereof and complementarily binds to the nucleic acid of human herpesvirus. A DNA chip for detecting human herpesvirus(HHV) or genotyping of HHV contains at least one probes selected from oligonucleotides having the nucleotide sequences of SEQ ID NO: 1 to SEQ ID NO: 9 and complementary oligonucleotides thereof. A genotyping kit for human herpesvirus comprises (a) the DNA chip for detecting human herpesvirus, (b) a PCR primer and (c) a marker means for detecting amplified DNA hybridized with the DNA chip. A genotyping method for human herpesvirus comprises the steps of: (a) amplifying a sample DNA using the PCR primer; (b) hybridizing the amplified DNA on the DNA chip containing one probe or two or more of probe sets; and (c) detecting the product hybridized with the probe.

Abstract translation: 目的：提供用于检测人疱疹病毒（HHV）的探针，基因分型试剂盒和使用其的人疱疹病毒的基因分型方法，从而快速，简单且准确地检测由单纯疱疹病毒1型（HSV-1）组成的HHV， HSV-2和人疱疹病毒-8（HHV-8），并导致STD（性传播疾病）。 构成：用于检测人疱疹病毒（HHV）的探针选自具有SEQ ID NO：1至SEQ ID NO：9的核苷酸序列的寡核苷酸及其互补寡核苷酸，并与人疱疹病毒的核酸互补结合。 用于检测人疱疹病毒（HHV）或HHV基因分型的DNA芯片含有选自具有SEQ ID NO：1至SEQ ID NO：9的核苷酸序列的寡核苷酸及其互补寡核苷酸的至少一种探针。 用于人疱疹病毒的基因分型试剂盒包括（a）用于检测人疱疹病毒的DNA芯片，（b）PCR引物和（c）用于检测与DNA芯片杂交的扩增DNA的标记方法。 人疱疹病毒的基因分型方法包括以下步骤：（a）使用PCR引物扩增样品DNA; （b）将包含一个探针或两个或更多个探针组的DNA芯片上的扩增DNA杂交; 和（c）检测与探针杂交的产物。