公开(公告)号：KR1020010091450A

公开(公告)日：2001-10-23

申请号：KR1020000013161

申请日：2000-03-15

Applicant: 안국바이오진단(주)

Inventor： 김성근 ,  김진희 ,  박태신 ,  박미선

IPC: C12Q1/68

CPC classification number: C12Q1/708 ,  C12Q1/6886

Abstract: PURPOSE: Provided is a diagnosis kit article for the genotype of human papillomavirus(HPV) which is a main cause of cervical dysplasia. And its manufacturing method is also provided. CONSTITUTION: The kit article consists of an oligonucleotide microarray manufactured by fixing HPV type specific oligonucleotide probes onto a glass, silylated slide, by Schiff base reaction, and streptavidine-R-phycoerythrin containing biotin-binding proteins to detect the hybridization reaction of biotin labeled DNA ampliers and the probe oligomers fixed on the glass. Its manufacturing method comprises the steps of: modifying HPV type specific DNA probes with an amine group; inducing an aldehyde group on a glass; fixing the modified DNA probes to the glass by Schiff base reaction.

Abstract translation: 目的：提供人乳头状瘤病毒（HPV）基因型的诊断试剂盒文章，这是宫颈发育不良的主要原因。 并提供其制造方法。 构成：试剂盒文章由通过将HPV型特异性寡核苷酸探针固定在玻璃，甲硅烷基化载玻片上，通过席夫碱反应制造的寡核苷酸微阵列和含有生物素结合蛋白的链霉亲和素-R-藻红蛋白来检测生物素标记的DNA的杂交反应 放大器和固定在玻璃上的探针低聚物。 其制备方法包括：用胺基改性HPV型特异性DNA探针; 在玻璃上诱导醛基; 通过席夫碱反应将修饰的DNA探针固定在玻璃上。

公开(公告)号：KR100452163B1

公开(公告)日：2004-10-12

申请号：KR1020010056827

申请日：2001-09-14

Applicant: 안국바이오진단(주)

Inventor： 윤성욱 ,  박태신 ,  김정미 ,  박미선

IPC: C12Q1/68

CPC classification number: C12Q1/708 ,  C12Q1/6837

Abstract: The present invention relates to a genotyping kit for diagnosis of detecting the human papillomavirus (HPV) infection, probes for genotyping the HPV, and DNA chips including the probes. Also, the present invention relates to a method for diagnosis of HPV infection.

Abstract translation: 本发明涉及用于诊断检测人乳头瘤病毒（HPV）感染的基因分型试剂盒，用于对HPV进行基因分型的探针，以及包含所述探针的DNA芯片。 而且，本发明涉及用于诊断HPV感染的方法。

公开(公告)号：KR1020030027178A

公开(公告)日：2003-04-07

申请号：KR1020010056827

申请日：2001-09-14

Applicant: 안국바이오진단(주)

Inventor： 윤성욱 ,  박태신 ,  김정미 ,  박미선

IPC: C12Q1/68

CPC classification number: C12Q1/708 ,  C12Q1/6837

Abstract: PURPOSE: Provided is a genotyping kit for diagnosis of human papilloma virus(HPV) infection by using probes which bind to human papilloma virus DNA and a DNA chip containing the probes, thereby diagnosing human papilloma virus infection rapidly and simply with high specificity and sensitivity and consequently prognosing, preventing and treating cervix cancer. CONSTITUTION: The genotyping kit for diagnosis of human papilloma virus(HPV) infection contains probes, which are selected from oligonucleotides having nucleotide sequences of SEQ ID NO:1 to 30 and complementary oligonucleotides, and bind to HPV DNA complementarily, and a DNA chip which contains at least one prove selected from the HPV probes.

Abstract translation: 目的：提供用于通过使用结合人乳头状瘤病毒DNA的探针和含有探针的DNA芯片来诊断人乳头状瘤病毒（HPV）感染的基因分型试剂盒，从而快速且简单地以高特异性和灵敏度诊断人乳头状瘤病毒感染， 因此预测，预防和治疗子宫颈癌。 构成：用于诊断人乳头瘤病毒（HPV）感染的基因分型试剂盒含有探针，其选自具有SEQ ID NO：1至30的核苷酸序列的寡核苷酸和互补寡核苷酸，并互补结合HPV DNA的DNA芯片， 包含从HPV探针中选出的至少一种证据。

公开(公告)号：KR3003024970000S

公开(公告)日：2002-07-18

申请号：KR3020010019059

申请日：2001-07-11

Applicant: 안국바이오진단(주)

Designer： 박태신

公开(公告)号：KR100382703B1

公开(公告)日：2003-05-09

申请号：KR1020000013161

申请日：2000-03-15

Applicant: 안국바이오진단(주)

Inventor： 김성근 ,  김진희 ,  박태신 ,  박미선

IPC: C12Q1/68

CPC classification number: C12Q1/708 ,  C12Q1/6886

Abstract: The present invention relates to a genotyping kit for diagnosing patients infected with human papillomavirus (HPV), and a method for diagnosis of HPV infection by genotyping specimen DNA isolated from the patients using the said kit. The genotyping kit of the invention comprises a DNA chip with probes that have nucleotide sequences complementary to DNA of HPV, primers for amplifying the DNA of a sample by PCR, and means for labeling hybridized sample DNA with the DNA chip. The method for diagnosis of HPV infection comprises the steps of amplifying DNA obtained from a sample using the primers of the kit, applying the amplified DNA to the DNA chip and hybridizing the amplified DNA and the probes of the DNA chip, and detecting DNA bound on the surface of the DNA chip by labeling hybridized DNA. In accordance with the invention, the genotyping kit may be practically applied to the early diagnosis, prevention and treatment of cervical cancer, since the kit can easily diagnose HPV infection, and can exactly determine the genotype of the HPV.

Abstract translation: 本发明涉及用于诊断感染人乳头瘤病毒（HPV）的患者的基因分型试剂盒以及通过使用所述试剂盒从患者分离的样本DNA的基因型分型来诊断HPV感染的方法。 本发明的基因分型试剂盒包括具有与HPV的DNA互补的核苷酸序列的探针的DNA芯片，通过PCR扩增样品的DNA的引物，以及用DNA芯片标记杂交的样品DNA的装置。 诊断HPV感染的方法包括以下步骤：使用试剂盒的引物扩增从样品获得的DNA，将扩增的DNA应用于DNA芯片，并将扩增的DNA与DNA芯片的探针杂交，并检测结合于 DNA芯片的表面通过标记杂交的DNA。 根据本发明，基因分型试剂盒可以实际应用于宫颈癌的早期诊断，预防和治疗，因为该试剂盒可以容易地诊断HPV感染，并且可以准确地确定HPV的基因型。