公开(公告)号：KR100464259B1

公开(公告)日：2005-01-03

申请号：KR1020020039464

申请日：2002-07-08

Applicant: 안국바이오진단(주) ,  재단법인한국간연구재단

Inventor： 김범준 ,  조문제 ,  국윤호 ,  김정미 ,  박정민

IPC: C12Q1/68

Abstract: PURPOSE: An identification method of Streptomyces having rifampin resistance or sensitivity using rpoB gene, and a primer specific to Streptomyces having rifampin resistance or sensitivity are provided, thereby simplifying the method, decreasing the costs therefor and improving the accuracy thereof. CONSTITUTION: An identification method of Streptomyces having rifampin resistance or sensitivity is characterized by using the nucleotide sequence difference encoding an amino acid of a rpoB gene of Streptomyces sp. corresponding to the 352th amino acid in RNA polymerase subunit B of Streptomyces coelicolor, wherein the nucleotide sequence encoding the amino acid is 258-bp to 260-bp of nucleotide sequence from the 5-terminal of a 352-bp fragment obtained by amplifying the rpoB gene of Streptomyces sp. using a pair of primers having the nucleotide sequences of SEQ ID NO: 1 and SEQ ID NO: 2.

Abstract translation: 目的：提供使用rpoB基因的具有利福平抗性或灵敏度的链霉菌的鉴定方法，以及对具有利福平耐受性或灵敏度的链霉菌特异性的引物，从而简化了方法，降低了成本并提高了其准确度。 构成：具有利福平抗性或敏感性的链霉菌的鉴定方法的特征在于使用编码链霉菌属的rpoB基因的氨基酸的核苷酸序列差异。 其对应于天蓝色链霉菌的RNA聚合酶亚基B中的第352位氨基酸，其中编码该氨基酸的核苷酸序列是从扩增rpoB获得的352-bp片段的5'端的核苷酸序列的258-bp至260-bp 链霉菌属的基因。 使用具有SEQ ID NO：1和SEQ ID NO：2的核苷酸序列的一对引物。

公开(公告)号：KR1020040005159A

公开(公告)日：2004-01-16

申请号：KR1020020039464

申请日：2002-07-08

Applicant: 안국바이오진단(주) ,  재단법인한국간연구재단

Inventor： 김범준 ,  조문제 ,  국윤호 ,  김정미 ,  박정민

IPC: C12Q1/68

Abstract: PURPOSE: An identification method of Streptomyces having rifampin resistance or sensitivity using rpoB gene, and a primer specific to Streptomyces having rifampin resistance or sensitivity are provided, thereby simplifying the method, decreasing the costs therefor and improving the accuracy thereof. CONSTITUTION: An identification method of Streptomyces having rifampin resistance or sensitivity is characterized by using the nucleotide sequence difference encoding an amino acid of a rpoB gene of Streptomyces sp. corresponding to the 352th amino acid in RNA polymerase subunit B of Streptomyces coelicolor, wherein the nucleotide sequence encoding the amino acid is 258-bp to 260-bp of nucleotide sequence from the 5-terminal of a 352-bp fragment obtained by amplifying the rpoB gene of Streptomyces sp. using a pair of primers having the nucleotide sequences of SEQ ID NO: 1 and SEQ ID NO: 2.

Abstract translation: 目的：提供使用rpoB基因具有利福平抗性或灵敏度的链霉菌的鉴定方法，以及具有利福平抗性或灵敏度的链霉菌特异性引物，从而简化方法，降低其成本并提高其准确度。 构成：具有利福平抗性或灵敏度的链霉菌的鉴定方法的特征在于使用编码链霉菌rpoB基因的氨基酸的核苷酸序列差异。 对应于天蓝色链霉菌的RNA聚合酶亚基B中的第352位氨基酸，其中编码氨基酸的核苷酸序列是从通过扩增rpoB获得的352bp片段的5个末端的碱基序列的258bp至260bp 链霉菌属的基因 使用具有SEQ ID NO：1和SEQ ID NO：2的核苷酸序列的一对引物。

公开(公告)号：KR1020030015124A

公开(公告)日：2003-02-20

申请号：KR1020020036731

申请日：2002-06-28

Applicant: 안국바이오진단(주) ,  김범준 ,  국윤호

Inventor： 김범준 ,  김정미 ,  국윤호 ,  이근화 ,  천종식 ,  배경숙

IPC: C12Q1/68

Abstract: PURPOSE: A primer set specific to genus Streptomyces, rpoB gene fractions, and an identification method of genus Streptomyces by using the same are provided, thereby identifying the genus Streptomyces easily and cheaply. CONSTITUTION: The primer set specific to genus Streptomyces is selected from the group consisting of primers having the nucleotide sequences of SEQ ID NOs: 1 and 2, specifically amplifying RNA polymerase B(rpoB) subunit gene. The polynucleotide is selected from the nucleotide sequences of SEQ ID NOs: 3 to 164 and comprises 306 bp of the rpoB gene fraction of the genus Streptomyces. The identification method of genus Streptomyces comprises the steps of: (i) amplifying the rpoB gene fraction of a strain using the primer set specific to the genus Streptomyces; (ii) sequencing the amplified ropB gene fractions; and (iii) comparing the nucleotide sequences analyzed in the step (ii) and the rpoB gene sequence.

Abstract translation: 目的：提供特异性链霉菌属的引物组，rpoB基因级分，以及通过使用该引物对链霉菌属的鉴定方法，从而易于且便宜地鉴定链霉菌属。 构成：特异于链霉菌属的引物组选自具有SEQ ID NO：1和2的核苷酸序列的引物组，特异性扩增RNA聚合酶B（rpoB）亚基基因。 多核苷酸选自SEQ ID NO：3至164的核苷酸序列，并且包含306bp的链霉菌属的rpoB基因级分。 链霉菌属的鉴定方法包括以下步骤：（i）使用特异于链霉菌属的引物组扩增菌株的rpoB基因级分; （ii）测序扩增的ropB基因级分; 和（iii）比较步骤（ii）中分析的核苷酸序列和rpoB基因序列。

公开(公告)号：KR100464260B1

公开(公告)日：2005-01-03

申请号：KR1020020036731

申请日：2002-06-28

Applicant: 안국바이오진단(주) ,  김범준 ,  국윤호

Inventor： 김범준 ,  김정미 ,  국윤호 ,  이근화 ,  천종식 ,  배경숙

IPC: C12Q1/68

Abstract: PURPOSE: A primer set specific to genus Streptomyces, rpoB gene fractions, and an identification method of genus Streptomyces by using the same are provided, thereby identifying the genus Streptomyces easily and cheaply. CONSTITUTION: The primer set specific to genus Streptomyces is selected from the group consisting of primers having the nucleotide sequences of SEQ ID NOs: 1 and 2, specifically amplifying RNA polymerase B(rpoB) subunit gene. The polynucleotide is selected from the nucleotide sequences of SEQ ID NOs: 3 to 164 and comprises 306 bp of the rpoB gene fraction of the genus Streptomyces. The identification method of genus Streptomyces comprises the steps of: (i) amplifying the rpoB gene fraction of a strain using the primer set specific to the genus Streptomyces; (ii) sequencing the amplified ropB gene fractions; and (iii) comparing the nucleotide sequences analyzed in the step (ii) and the rpoB gene sequence.

Abstract translation: 目的：提供特异于链霉菌属，rpoB基因部分的引物组，以及通过使用它们的链霉菌属的鉴定方法，从而容易且廉价地鉴定链霉菌属。 构成：对链霉菌属特异性的引物组选自具有SEQ ID NO：1和2的核苷酸序列的引物，特别是扩增RNA聚合酶B（rpoB）亚基基因。 多核苷酸选自SEQ ID NO：3至164的核苷酸序列，并且包含链霉菌属的rpoB基因部分的306bp。 链霉菌属的鉴定方法包括以下步骤：（i）使用对链霉菌属特异性的引物组扩增菌株的rpoB基因组分; （ii）对扩增的ropB基因部分进行测序; 和（iii）比较步骤（ii）中分析的核苷酸序列和rpoB基因序列。