公开(公告)号：KR100464259B1

公开(公告)日：2005-01-03

申请号：KR1020020039464

申请日：2002-07-08

Applicant: 안국바이오진단(주) ,  재단법인한국간연구재단

Inventor： 김범준 ,  조문제 ,  국윤호 ,  김정미 ,  박정민

IPC: C12Q1/68

Abstract: PURPOSE: An identification method of Streptomyces having rifampin resistance or sensitivity using rpoB gene, and a primer specific to Streptomyces having rifampin resistance or sensitivity are provided, thereby simplifying the method, decreasing the costs therefor and improving the accuracy thereof. CONSTITUTION: An identification method of Streptomyces having rifampin resistance or sensitivity is characterized by using the nucleotide sequence difference encoding an amino acid of a rpoB gene of Streptomyces sp. corresponding to the 352th amino acid in RNA polymerase subunit B of Streptomyces coelicolor, wherein the nucleotide sequence encoding the amino acid is 258-bp to 260-bp of nucleotide sequence from the 5-terminal of a 352-bp fragment obtained by amplifying the rpoB gene of Streptomyces sp. using a pair of primers having the nucleotide sequences of SEQ ID NO: 1 and SEQ ID NO: 2.

Abstract translation: 目的：提供使用rpoB基因的具有利福平抗性或灵敏度的链霉菌的鉴定方法，以及对具有利福平耐受性或灵敏度的链霉菌特异性的引物，从而简化了方法，降低了成本并提高了其准确度。 构成：具有利福平抗性或敏感性的链霉菌的鉴定方法的特征在于使用编码链霉菌属的rpoB基因的氨基酸的核苷酸序列差异。 其对应于天蓝色链霉菌的RNA聚合酶亚基B中的第352位氨基酸，其中编码该氨基酸的核苷酸序列是从扩增rpoB获得的352-bp片段的5'端的核苷酸序列的258-bp至260-bp 链霉菌属的基因。 使用具有SEQ ID NO：1和SEQ ID NO：2的核苷酸序列的一对引物。

公开(公告)号：KR1020040005159A

公开(公告)日：2004-01-16

申请号：KR1020020039464

申请日：2002-07-08

Applicant: 안국바이오진단(주) ,  재단법인한국간연구재단

Inventor： 김범준 ,  조문제 ,  국윤호 ,  김정미 ,  박정민

IPC: C12Q1/68

Abstract: PURPOSE: An identification method of Streptomyces having rifampin resistance or sensitivity using rpoB gene, and a primer specific to Streptomyces having rifampin resistance or sensitivity are provided, thereby simplifying the method, decreasing the costs therefor and improving the accuracy thereof. CONSTITUTION: An identification method of Streptomyces having rifampin resistance or sensitivity is characterized by using the nucleotide sequence difference encoding an amino acid of a rpoB gene of Streptomyces sp. corresponding to the 352th amino acid in RNA polymerase subunit B of Streptomyces coelicolor, wherein the nucleotide sequence encoding the amino acid is 258-bp to 260-bp of nucleotide sequence from the 5-terminal of a 352-bp fragment obtained by amplifying the rpoB gene of Streptomyces sp. using a pair of primers having the nucleotide sequences of SEQ ID NO: 1 and SEQ ID NO: 2.

Abstract translation: 目的：提供使用rpoB基因具有利福平抗性或灵敏度的链霉菌的鉴定方法，以及具有利福平抗性或灵敏度的链霉菌特异性引物，从而简化方法，降低其成本并提高其准确度。 构成：具有利福平抗性或灵敏度的链霉菌的鉴定方法的特征在于使用编码链霉菌rpoB基因的氨基酸的核苷酸序列差异。 对应于天蓝色链霉菌的RNA聚合酶亚基B中的第352位氨基酸，其中编码氨基酸的核苷酸序列是从通过扩增rpoB获得的352bp片段的5个末端的碱基序列的258bp至260bp 链霉菌属的基因 使用具有SEQ ID NO：1和SEQ ID NO：2的核苷酸序列的一对引物。