公开(公告)号：KR100758727B1

公开(公告)日：2007-09-14

申请号：KR1020060030113

申请日：2006-04-03

Applicant: 재단법인서울대학교산학협력재단

Inventor： 김범준 ,  지영미 ,  김홍 ,  문호숙

IPC: C12Q1/68 ,  G01N33/576 ,  G01N33/53

CPC classification number: C12Q1/6883 ,  C12Q1/6827 ,  C12Q2600/118 ,  G01N33/5308 ,  G01N33/5761

Abstract: A composition for diagnosis of the progress of liver disease of a HBV(Hepatitis B Virus) infected patient and a kit containing the same composition are provided to anticipate, diagnose and prevent the progress of liver disease of the HBV infected patient by confirming nucleotide deletion of a preS1 initiation site. A polynucleotide which has base deletions of 15-21 bp(2848th to 2880th bases) in the coding initiation site of preS1 gene of HBV genotype C genome genes having the nucleotide sequence of SEQ ID NO:19, and total 15-400 continuous bases located at both ends of the deletion site is provided, wherein the base deletion is 15bp base deletion from 2849th-2863th bases in the nucleotide sequence of SEQ ID NO:19, 15bp base deletion from 2850th-2864th bases in the nucleotide sequence of SEQ ID NO:19, 21bp base deletion from 2849th-2869th bases in the nucleotide sequence of SEQ ID NO:19, or 21bp base deletion from 2854th-2874th bases in the nucleotide sequence of SEQ ID NO:19. The composition for diagnosis of the progress of liver disease of a HBV infected patient comprises the probe having a nucleotide sequence complementary with the polynucleotide sequence, selected from SEQ ID NOs:9, 10, 11 and 12 to detect the polynucleotide.

Abstract translation: 提供用于诊断HBV（乙型肝炎病毒）感染患者的肝病进展的组合物和含有相同组成的试剂盒，以通过确认HBV感染患者的肝脏疾病的核苷酸缺失来预测，诊断和预防HBV感染患者的肝病进展 preS1启动点。 在具有SEQ ID NO：19的核苷酸序列的HBV基因型C基因组基因的preS1基因的编码起始位点中具有15-21bp（2848至2880个碱基）的碱基缺失的多核苷酸，以及总共15-400个连续碱基 提供缺失位点的两端，其中碱基缺失是SEQ ID NO：19的核苷酸序列中的第2849位至第2863位碱基之间的15bp碱基缺失，SEQ ID NO：1的核苷酸序列中的第2850至第2864位碱基的15bp碱基缺失 SEQ ID NO：19的核苷酸序列中的第2849〜2869位碱基有21bp的碱基缺失或SEQ ID NO：19的核苷酸序列中第2854〜2874位碱基的21bp碱基缺失。 用于诊断HBV感染患者的肝病进展的组合物包括具有与选自SEQ ID NO：9,10,11和12的多核苷酸序列互补的核苷酸序列的探针，以检测多核苷酸。