公开(公告)号：KR100758727B1

公开(公告)日：2007-09-14

申请号：KR1020060030113

申请日：2006-04-03

Applicant: 재단법인서울대학교산학협력재단

Inventor： 김범준 ,  지영미 ,  김홍 ,  문호숙

IPC: C12Q1/68 ,  G01N33/576 ,  G01N33/53

CPC classification number: C12Q1/6883 ,  C12Q1/6827 ,  C12Q2600/118 ,  G01N33/5308 ,  G01N33/5761

Abstract: A composition for diagnosis of the progress of liver disease of a HBV(Hepatitis B Virus) infected patient and a kit containing the same composition are provided to anticipate, diagnose and prevent the progress of liver disease of the HBV infected patient by confirming nucleotide deletion of a preS1 initiation site. A polynucleotide which has base deletions of 15-21 bp(2848th to 2880th bases) in the coding initiation site of preS1 gene of HBV genotype C genome genes having the nucleotide sequence of SEQ ID NO:19, and total 15-400 continuous bases located at both ends of the deletion site is provided, wherein the base deletion is 15bp base deletion from 2849th-2863th bases in the nucleotide sequence of SEQ ID NO:19, 15bp base deletion from 2850th-2864th bases in the nucleotide sequence of SEQ ID NO:19, 21bp base deletion from 2849th-2869th bases in the nucleotide sequence of SEQ ID NO:19, or 21bp base deletion from 2854th-2874th bases in the nucleotide sequence of SEQ ID NO:19. The composition for diagnosis of the progress of liver disease of a HBV infected patient comprises the probe having a nucleotide sequence complementary with the polynucleotide sequence, selected from SEQ ID NOs:9, 10, 11 and 12 to detect the polynucleotide.

Abstract translation: 提供用于诊断HBV（乙型肝炎病毒）感染患者的肝病进展的组合物和含有相同组成的试剂盒，以通过确认HBV感染患者的肝脏疾病的核苷酸缺失来预测，诊断和预防HBV感染患者的肝病进展 preS1启动点。 在具有SEQ ID NO：19的核苷酸序列的HBV基因型C基因组基因的preS1基因的编码起始位点中具有15-21bp（2848至2880个碱基）的碱基缺失的多核苷酸，以及总共15-400个连续碱基 提供缺失位点的两端，其中碱基缺失是SEQ ID NO：19的核苷酸序列中的第2849位至第2863位碱基之间的15bp碱基缺失，SEQ ID NO：1的核苷酸序列中的第2850至第2864位碱基的15bp碱基缺失 SEQ ID NO：19的核苷酸序列中的第2849〜2869位碱基有21bp的碱基缺失或SEQ ID NO：19的核苷酸序列中第2854〜2874位碱基的21bp碱基缺失。 用于诊断HBV感染患者的肝病进展的组合物包括具有与选自SEQ ID NO：9,10,11和12的多核苷酸序列互补的核苷酸序列的探针，以检测多核苷酸。

公开(公告)号：KR1020080047269A

公开(公告)日：2008-05-28

申请号：KR1020070115941

申请日：2007-11-14

Applicant: 재단법인서울대학교산학협력재단 ,  재단법인 제주테크노파크

Inventor： 김범준 ,  문호숙 ,  오은주 ,  김창진

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6869

Abstract: A method for identifying a pathogenic genus of potato scab is provided to identify the pathogenic genus conveniently and economically with high accuracy by solving problems of a generally used 16S rRNA identification technique and an identification method targeting 16S-23S ITS, thereby being widely used for identifying the pathogenic genus of the potato scab. A method for identifying a pathogenic genus of potato scab comprises the steps of: (a) amplifying an rpoB gene fragment of a target strain using at least one primer selected from the group consisting of a primer including a sequence of SEQ ID : NO. 1 and a primer including a sequence of SEQ ID : NO. 2; (b) analysing the sequence of the amplified rpoB gene fragment; and (c) after multialigning the sequence analyzed by the step(b) by substituting it for a sequence of an rpoB gene selected from the group consisting of sequences of SEQ ID : NOs. 3 to 21 of a standard strain related to the potato scab, completing a genealogy to determine the genus. A method for classifying the pathogenic genus of the potato scab comprises a step of analyzing signature amino acid sequences of 336th and 442nd among an amino acid deduced from the rpoB gene sequence analyzed by the step(b).

Abstract translation: 提供了一种鉴定马铃薯斑纹病病原体的方法，通过解决一般使用的16S rRNA鉴定技术和针对16S-23S ITS的鉴定方法的问题，以高精度方便，经济地识别病原属，从而广泛用于鉴定 马铃薯痂病的病原属。 用于鉴定马铃薯斑纹病的致病属的方法包括以下步骤：（a）使用至少一种选自下组的引物扩增靶菌株的rpoB基因片段：包括SEQ ID NO： 1和包含SEQ ID NO：1的序列的引物。 2; （b）分析扩增的rpoB基因片段的序列; 和（c）通过用步骤（b）分析的序列多重标记选自SEQ ID NO：的序列的rpoB基因的序列后。 3至21个与马铃薯痂病相关的标准菌株，完成家谱以确定属。 用于分类马铃薯斑纹病的致病属的方法包括分析由步骤（b）分析的rpoB基因序列推导的氨基酸中第336位和第442位的特征氨基酸序列的步骤。

公开(公告)号：KR100959195B1

公开(公告)日：2010-05-24

申请号：KR1020070115941

申请日：2007-11-14

Applicant: 재단법인서울대학교산학협력재단 ,  재단법인 제주테크노파크

Inventor： 김범준 ,  문호숙 ,  오은주 ,  김창진

IPC: C12Q1/68

Abstract: 본 발명은
rpoB 유전자를 이용한 감자 더뎅이 병원성 균종의 동정방법에 관한 것으로서, 더욱 상세하게는 모든 스크렙토마이세스 속 균종의
rpoB 유전자를 증폭시킬 수 있는 특이적 프라이머를 이용하여 감자 더뎅이병과 연관되어 있다고 알려진 표준 균주의
rpoB 유전자를 증폭시킨 후 염기서열을 분석하여 데이터베이스를 구축하고, 이 데이터베이스로 기존의 형태학적 분류법이 갖는 시간, 정확성의 문제점을 해소하며, 또한 요즘 일반적으로 사용되고 있는 16S rDNA 동정법의 갖는 시간, 비용 상의 문제점을 해결하여 간편하고, 경제적이며 정확성이 높은 감자 더뎅이 병 균종을 동정하는 방법에 관한 것이다.
또한, 본 발명은 분석된
rpoB 유전자 염기서열의 추론된(deduced) 아미노산 중 336 번째와 442 번째의 시그네이쳐(signature) 아미노산 염기서열을 분석하여 감자더뎅이 병원성 균종으로 분류하는 방법을 개발함으로써 이 균종의 동정 및 감자더뎅이병 진단에 유용하리라 기대된다.
rpoB 유전자, 감자 더뎅이, 특이적 프라이머, 동정 방법, 336 번째와 442 번째의 시그네이쳐(signature) 아미노산 염기서열