公开(公告)号：KR100781059B1

公开(公告)日：2007-11-30

申请号：KR1020060120463

申请日：2006-12-01

Applicant: 재단법인서울대학교산학협력재단

Inventor： 정종주 ,  정춘균 ,  김정호 ,  송상익 ,  최양도

IPC: C12N15/29 ,  C12N15/63

Abstract: A method for preparing a transformed plant using a P_AtMYB44 promoter derived from Arabidopsis thaliana is provided to obtain the plant specifically expressing a target protein in a guard cell under various environmental stresses such as moisture deficiency, high salinity, and low temperature without influencing on the ordinary growth characteristics of the plant by linking the promoter to a target gene and then transforming the plant using the same. An environmental stress inducible P_AtMYB44 promoter derived from Arabidopsis thaliana includes a sequence described in SEQ ID : NO.1, wherein the environmental stress is drought, salt damage or cold-weather damage. A recombinant plant expression vector includes the promoter and is prepared by linking a target gene encoding a target protein to downstream of the promoter. A method for producing a target protein in a plant comprises the steps of: (a) transforming a plant using the recombinant plant expression vector; and (b) applying at least one environmental stress selected from the group consisting of drought, salt damage and cold-weather damage to the transformed plant. A method for preparing a transformed plant capable of specifically expressing a target protein in a guard cell comprises a step of transforming the recombinant plant expression vector into a plant.

Abstract translation: 提供使用来自拟南芥的P_AtMYB44启动子制备转化植物的方法，以在各种环境胁迫如水分不足，高盐度和低温下在保护细胞中特异性表达靶蛋白的植物，而不影响普通 通过将启动子与靶基因连接，然后使用其转化植物，植物的生长特性。 源自拟南芥的环境胁迫诱导型P_AtMYB44启动子包括SEQ ID NO：1所述的序列，其中环境胁迫是干旱，盐损害或寒冷天气损害。 重组植物表达载体包括启动子，并通过将编码靶蛋白的靶基因与启动子的下游连接来制备。 制备植物中靶蛋白的方法包括以下步骤：（a）使用重组植物表达载体转化植物; 和（b）将至少一种选自干旱，盐损害和寒冷天气损害的环境胁迫应用于转化植物。 制备能够在保护细胞中特异性表达靶蛋白质的转化植物的方法包括将重组植物表达载体转化到植物中的步骤。