公开(公告)号：KR100781070B1

公开(公告)日：2007-11-30

申请号：KR1020060120433

申请日：2006-12-01

Applicant: 재단법인서울대학교산학협력재단

Inventor： 정종주 ,  정춘균 ,  류승현 ,  박현진 ,  손황배 ,  최양도

IPC: C12N15/29

Abstract: A method for preparing a plant with increased disease-resistance is provided to obtain a plant having high resistance against pathogenic fungi by transforming a lectin gene AtMJI2 derived from Arabidopsis thaliana and expressed by chitin and jasmonate, thereby significantly decreasing the loss induced by the infection of blight and harmful insects. A method for increasing the disease resistance of a plant comprises a step of transforming a recombinant plant expression vector including a lectin gene AtMJI2 derived from Arabidopsis thaliana to a plant, wherein the vector is pCaAtMJ12 depicted in Fig. 4. An Arabidopsis thaliana prepared by the method is characterized in that it has the increased disease resistance.

Abstract translation: 提供了一种具有增加抗病性的植物的制备方法，通过转化来自拟南芥并由甲壳素和茉莉酮酸酯表达的凝集​​素基因AtMJI​​2，从而显着降低由感染引起的损失，从而获得具有高抗病原真菌的植物 枯萎和害虫。 提高植物抗病性的方法包括将包含来自拟南芥的凝集素基因AtMJI​​2的重组植物表达载体转化到植物中的步骤，其中载体是图1所示的pCaAtMJ12。 通过该方法制备的拟南芥的特征在于其具有增加的抗病性。

公开(公告)号：KR1020070076918A

公开(公告)日：2007-07-25

申请号：KR1020060006407

申请日：2006-01-20

Applicant: 재단법인서울대학교산학협력재단

Inventor： 정종주 ,  최양도 ,  정춘균 ,  구연종 ,  서준성

IPC: C12N15/29 ,  C12N15/11 ,  C12N15/10

Abstract: Provided is an AtMYB44 protein derived from Arabidopsis thaliana which is a transcription factor controlling the expression of other genes, is used for preparing a transformed plant which could effectively control the flowering time of the plant without influencing on general growth characteristics, and has high resistance on various stress such as low temperature, moisture deficiency and high salinity. The DNA coding a transcription factor AtMYB44 controlling the expression of genes derived from Arabidopsis thaliana and concerned with the stress-resistance and the development process has a nucleotide sequence described as SEQ ID : NO. 1 and an amino acid sequence described as SEQ ID : NO. 2. The binary vector pCaAtMYB44 for expressing plant genes is constructed by recombining the AtMYB44 genes with pBI111L. A transformed plant in which the AtMYB44 is over-expressed is prepared by transforming the AtMYB44 gene using a vector pCaAtMYB44.

Abstract translation: 提供了一种来自拟南芥属的AtMYB44蛋白，其是控制其他基因表达的转录因子，用于制备可以有效控制植物开花时间而不影响一般生长特性的转化植物，并具有高抗性 各种应力如低温，水分不足和盐度高。 编码转录因子AtMYB44的DNA，其控制来自拟南芥的基因的表达并涉及应激抗性和发育过程，具有如SEQ ID NO：1所示的核苷酸序列。 1和如SEQ ID NO：1所示的氨基酸序列。 用于表达植物基因的二元载体pCaAtMYB44通过将AtMYB44基因与pBI111L重组而构建。 通过使用载体pCaAtMYB44转化AtMYB44基因来制备AtMYB44过表达的转化植物。

公开(公告)号：KR100792169B1

公开(公告)日：2008-01-07

申请号：KR1020060006407

申请日：2006-01-20

Applicant: 재단법인서울대학교산학협력재단

Inventor： 정종주 ,  최양도 ,  정춘균 ,  구연종 ,  서준성

IPC: C12N15/29 ,  C12N15/11 ,  C12N15/10

Abstract: 본 발명은 애기장대에서 분리한 전사인자 AtMYB44의 유전자를 포함하는 재조합 DNA를 전이함으로써 제조된 형질전환 식물체에 관한 것이다. 상기 전사인자 단백질은 식물호르몬 앱사이식산(abscisic acid; ABA)에 의하여 활성화 되어, 식물의 생장 발달과정을 조절하거나 저수분, 고염, 저온 등 외부 환경스트레스에 대하여 저항성을 부여하는 유전자들의 발현을 유도한다. 그러므로 상기 신규 유전자를 식물체에 도입하여 과다 발현시킴으로써 개화지연 및 광엽화 등 식물의 발달과정이 조절되거나 가뭄, 염해, 냉해 등 각종 환경스트레스에 대한 저항성을 나타내는 형질전환 식물체를 얻을 수 있다.
전사인자, 앱사이식산, 식물 생장조절, 광엽화, 개화지연, 환경스트레스 저항성 식물

公开(公告)号：KR100781059B1

公开(公告)日：2007-11-30

申请号：KR1020060120463

申请日：2006-12-01

Applicant: 재단법인서울대학교산학협력재단

Inventor： 정종주 ,  정춘균 ,  김정호 ,  송상익 ,  최양도

IPC: C12N15/29 ,  C12N15/63

Abstract: A method for preparing a transformed plant using a P_AtMYB44 promoter derived from Arabidopsis thaliana is provided to obtain the plant specifically expressing a target protein in a guard cell under various environmental stresses such as moisture deficiency, high salinity, and low temperature without influencing on the ordinary growth characteristics of the plant by linking the promoter to a target gene and then transforming the plant using the same. An environmental stress inducible P_AtMYB44 promoter derived from Arabidopsis thaliana includes a sequence described in SEQ ID : NO.1, wherein the environmental stress is drought, salt damage or cold-weather damage. A recombinant plant expression vector includes the promoter and is prepared by linking a target gene encoding a target protein to downstream of the promoter. A method for producing a target protein in a plant comprises the steps of: (a) transforming a plant using the recombinant plant expression vector; and (b) applying at least one environmental stress selected from the group consisting of drought, salt damage and cold-weather damage to the transformed plant. A method for preparing a transformed plant capable of specifically expressing a target protein in a guard cell comprises a step of transforming the recombinant plant expression vector into a plant.

Abstract translation: 提供使用来自拟南芥的P_AtMYB44启动子制备转化植物的方法，以在各种环境胁迫如水分不足，高盐度和低温下在保护细胞中特异性表达靶蛋白的植物，而不影响普通 通过将启动子与靶基因连接，然后使用其转化植物，植物的生长特性。 源自拟南芥的环境胁迫诱导型P_AtMYB44启动子包括SEQ ID NO：1所述的序列，其中环境胁迫是干旱，盐损害或寒冷天气损害。 重组植物表达载体包括启动子，并通过将编码靶蛋白的靶基因与启动子的下游连接来制备。 制备植物中靶蛋白的方法包括以下步骤：（a）使用重组植物表达载体转化植物; 和（b）将至少一种选自干旱，盐损害和寒冷天气损害的环境胁迫应用于转化植物。 制备能够在保护细胞中特异性表达靶蛋白质的转化植物的方法包括将重组植物表达载体转化到植物中的步骤。