公开(公告)号：KR1020090030775A

公开(公告)日：2009-03-25

申请号：KR1020070096314

申请日：2007-09-21

Applicant: 재단법인서울대학교산학협력재단

Inventor： 주상우 ,  조근창 ,  이소영

IPC: G01N33/48 ,  G01N21/65 ,  B82Y15/00

Abstract: A monitoring and imaging method for cells is provided to enable an operator to observe processes of a drug and gene delivery carrier transferring or moving to a specific site of a cell for a long time when the drug and gene delivery carrier are injected to the cell. A monitoring and imaging method for cells uses a silver nanoparticle, gold nanoparticle, or a nanostructure by surface-enhanced Raman spectroscopy(SERS). The nanoparticle or nanostructure is a carrier used for drug or gene delivery. The nanoparticle or nanostructure is selected from the group consisting of isocyanide compounds, thiol or cyanide, isothiocyanate, and Rhodamine 6G, Cy3, Cy5 or TAMRA. The monitoring method takes place within 72 hours after the nanoparticle or nanostructure is absorbed in the cell.

Abstract translation: 提供了一种用于细胞的监测和成像方法，以便当药物和基因递送载体注入细胞时，操作者可以长时间观察转移或移动至细胞特定部位的药物和基因递送载体的过程。 细胞的监测和成像方法通过表面增强拉曼光谱（SERS）使用银纳米颗粒，金纳米颗粒或纳米结构。 纳米颗粒或纳米结构是用于药物或基因递送的载体。 纳米颗粒或纳米结构选自异氰化物化合物，硫醇或氰化物，异硫氰酸酯和罗丹明6G，Cy3，Cy5或TAMRA。 监测方法是在纳米颗粒或纳米结构被吸收到细胞后72小时内进行的。

公开(公告)号：KR100670590B1

公开(公告)日：2007-01-17

申请号：KR1020050093500

申请日：2005-10-05

Applicant: 주식회사 디지탈바이오테크놀러지 ,  재단법인서울대학교산학협력재단

Inventor： 장준근 ,  방현우 ,  윤호영 ,  조근창 ,  정찬일

IPC: G01N15/02 ,  G01N15/00

CPC classification number: G01N15/1404 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0652 ,  B01L2200/0694 ,  B01L2200/146 ,  B01L2400/049 ,  B01L2400/084 ,  G01N2015/1413

Abstract: A microchip with an expanded channel and a fine particle-analyzing apparatus using the same are provided to increase the working amount of the sample processing by placing the focusing channel even if the speed of the sample and the sheath flows is increased. A microchip comprises a sample channel where the sample is flowed; a sheath flow channel where the sheath flow is flowed; a focusing channel where the sample particles sheath and focused with the sample and the sheath flow are flowed; and an expansion channel for expanding the point circumference of the focusing channel where the sample is analyzed. The focused sample particles keep the focusing status, lower the speed, and improve the detection effect of the sample particles when the focused sample particles pass through the expansion channel.

Abstract translation: 提供具有扩展通道的微芯片和使用该微芯片的微粒分析装置，即使样品和护套流动的速度增加，通过放置聚焦通道来增加样品处理的工作量。 微芯片包括样品流动的样品通道; 护套流动的护套流动通道; 样品颗粒鞘并与样品和护套流动聚焦的聚焦通道; 以及用于扩大分析样品的聚焦通道的点圆周的扩展通道。 当聚焦的样品颗粒通过扩展通道时，聚焦的样品颗粒保持聚焦状态，降低速度，并提高样品颗粒的检测效果。