Abstract:
This invention involves an apparatus of outlet reservoir and a technique for performing large volume stacking using electroosmotic flow pump (LVSEP) in capillary electrophoresis-mass spectrometry (CE-MS), which is a simple method to achieve greater enhancement in detection sensitivity. This invention utilizes an apparatus which is placed between MS orifice and capillary outlet and allows to supply the flow of the run buffer in the direction opposite to sample injection during sample stacking. The capillary outlet and electrospray ionization emitter are immersed into the apparatus which is filled with the run buffer. This apparatus is in a manner that attachment and detachment are allowed between MS orifice and capillary outlet.
Abstract:
본 발명은 DNA 칩의 DNA 혼성화(hybridization) 측정방법에 관한 것으로서, 보다 상세하게는 탐침 나노파티클 DNA(probe nanoparticle DNA)를 이용한 DNA 칩의 빛의 산란 정도를 측정하는 것을 특징으로 하는 상기 DNA 칩의 DNA 혼성화 측정방법에 관한 것이다. 상기 DNA 혼성화 측정방법은 DNA 칩의 혼성화를 측정하는 새로운 검출방법으로 형광물질의 표지나 그에 따른 별도의 고가의 광학기계 없이 저가의 램프가 설치된 스캐너 등으로 DNA 칩의 DNA 혼성화 정도를 손쉽게 확인할 수 있게 되어 DNA 칩의 활용을 용이하게 하여 DNA 칩의 활성화에 기여한다. DNA 칩, 혼성화, 산란현상
Abstract:
A sample concentration method using a real-time automatic elution solid phase extraction method combined with non-aqueous capillary electrophoresis and an interface structure of capillary tube and solid phase extraction device for the method are provided to analyze a sample of very low concentration by desorbing the analyzed material which is adsorbed in the filler with a large amount of elution solvent and concentrating it. A sample concentration method using a real-time automatic elution solid phase extraction method combined with non-aqueous capillary electrophoresis comprises a step of extracting a sample on a solid phase, a step of injecting elution solvent for desorbing the analyzed material, which is absorbed in the filler of the solid phase extraction device, into the end of a capillary tube, and a step of pushing the elution solvent to the filler by injecting non-aqueous buffer solution into the end of the capillary tube.
Abstract:
A method for detecting DNA hybridization of a DNA chip is provided to improve detection convenience by using scattering phenomenon through an inexpensive scanner instead of labeling of fluorescent material and expensive optical devices therefor, so that the availability of the DNA chip is improved. A DNA hybridization of a DNA chip is detected by measuring the light scattering degree of the DNA chip by using a probe nanoparticle DNA with a laser scanner or digital camera. A method for detecting DNA hybridization of a DNA chip comprises the steps of: reacting the capture DNA of the DNA chip for inspection with a probe nanoparticle DNA to induce hybridization; (ii) removing the unhybridized probe nanoparticle DNA; and (iii) measuring the light scattering degree of the DNA chip, wherein the nanoparticle is made of silica, gold, polystyrene or quantum dot and contains a fluorescent material TMR(tetramethyl rhodamine) or radioactive isotope in the inside or on the outside surface.
Abstract:
An experimental apparatus for measuring molarity or normality is provided to produce solution with the concentration proximate to a target level by correcting a mass measurement error by regulating the volume. An experimental apparatus for measuring molarity or normality is composed of a reference mark line(15) for measuring molarity or normality and indication scales(21,22,23,31,32,33) marked at the upper end of the reference mark line according to one of groups for decrease concentration intervals of 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, and 10% and marked at the lower end of the reference mark line according to one of groups for increase concentration intervals of 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, and 10%.
Abstract:
본 발명은 본 발명은 폴리에틸렌글라이콜(PEG)이 결합된 기능성 실리카 나노파티클 및 그 제조방법에 관한 것으로서, 보다 상세하게는 일반적인 나노파티클에 PEG기를 붙여서, 나노파티클의 뭉침현상을 방지함과 아울러 세포에 결합할 리간드부분 전에 PEG를 붙임으로써 반응성을 매우 높은 기능성 실리카 나노파티클 및 그 제조방법에 관한 것이다. 본 발명은 실리카 나노파티클 표면에 아민기가 결합되고, 상기 아민기에 PEG가 결합된 기능성 실리카 나노파티클 및 상기 실리카 나노파티클에 추가적으로 폴레이트(folate)가 결합되거나 상기 폴레이트는 PEG에 결합되는 것을 특징으로 하는 기능성 실리카 나노파티클을 제공한다. 또한, ⅰ) 역 마이크로에멀젼 방법에 의한 통상의 염료-나노파티클의 합성 단계; ⅱ) 상기 ⅰ)의 나노파티클을 아민그룹으로 표면처리하는 단계; ⅲ) 아민그룹에 PEG를 도입하는 단계를 포함하는 상기의 기능성 실리카 나노파티클의 제조방법을 제공하며, 상기 ⅲ) 단계 이후에 폴레이트를 결합시키는 단계를 포함할 수 있다. 나노파티클, 뭉침현상, 반응성, PEG
Abstract:
A functionalized silica nanoparticle and a method for preparing the same are provided to show reduced agglutination and non-specific linkage of nanoparticles. A functionalized silica nanoparticle comprises a silica nanoparticle and an amine group linked to a surface of the silica nanoparticle, wherein polyethylene glycol(PEG) is linked to the amine group. Additionally, folate is linked to the PEG of the silica nanoparticle. The functionalized silica nanoparticle is prepared by a method comprising the steps of: i) synthesizing a conventional dye-nanoparticle by reverse microemulsion process; ii) surface-treating the nanoparticle with an amine group; iii) introducing PEG to the amine group; and optionally iv) linking the folate thereto.
Abstract:
본 발명은 모세관 전기영동에서 큰 부피 단일 방울 미세추출방법과 스위핑 방법을 결합한 시료의 이중 농축 방법과 큰 부피 단일 방울 미세 추출을 위한 모세관에 관한 것이다. 보다 상세하게는 모세관 전기영동에서 모세관의 말단에 미세방울을 형성하여 액체상 미세추출을 함에 있어 미세 방울을 안정적으로 형성함과 아룰러 큰 부피로 형성할 수 있는 방법과 이를 스위핑 방법과 결합하여 적은 양의 시료나 극히 낮은 농도의 시료를 선 농축하여 이의 분석시 그 감도를 매우 증진시키는 기술에 관한 것이다. 모세관 전기영동, 단일 방울 미세추출, 스태킹, 감도
Abstract:
A method for performing single drop microextraction is provided to form microdroplet steadily in capillary electrophoresis and concentrating the sample. A method for performing single drop microextraction by forming microdroplet at the end of the capillary tube for preconcentration of the sample in capillary electrophoresis, comprises a step for inserting hydrophobic tube at the end of the capillary tube to form single drop stably. The tube is selected from the group consisting of polytetrafluoroethylene, perfluoroalkoxy polymer resin, fluorinated ethylenepropylene and polyetheretherketones.