Abstract:
본 발명은 DNA 칩의 DNA 혼성화(hybridization) 측정방법에 관한 것으로서, 보다 상세하게는 탐침 나노파티클 DNA(probe nanoparticle DNA)를 이용한 DNA 칩의 빛의 산란 정도를 측정하는 것을 특징으로 하는 상기 DNA 칩의 DNA 혼성화 측정방법에 관한 것이다. 상기 DNA 혼성화 측정방법은 DNA 칩의 혼성화를 측정하는 새로운 검출방법으로 형광물질의 표지나 그에 따른 별도의 고가의 광학기계 없이 저가의 램프가 설치된 스캐너 등으로 DNA 칩의 DNA 혼성화 정도를 손쉽게 확인할 수 있게 되어 DNA 칩의 활용을 용이하게 하여 DNA 칩의 활성화에 기여한다. DNA 칩, 혼성화, 산란현상
Abstract:
A method for detecting DNA hybridization of a DNA chip is provided to improve detection convenience by using scattering phenomenon through an inexpensive scanner instead of labeling of fluorescent material and expensive optical devices therefor, so that the availability of the DNA chip is improved. A DNA hybridization of a DNA chip is detected by measuring the light scattering degree of the DNA chip by using a probe nanoparticle DNA with a laser scanner or digital camera. A method for detecting DNA hybridization of a DNA chip comprises the steps of: reacting the capture DNA of the DNA chip for inspection with a probe nanoparticle DNA to induce hybridization; (ii) removing the unhybridized probe nanoparticle DNA; and (iii) measuring the light scattering degree of the DNA chip, wherein the nanoparticle is made of silica, gold, polystyrene or quantum dot and contains a fluorescent material TMR(tetramethyl rhodamine) or radioactive isotope in the inside or on the outside surface.
Abstract:
PURPOSE: A composite is provided to have ion conductivity and moisturizing ability, thereby providing excellent proton conductivity with a composite film. CONSTITUTION: A composite is represented by chemical formula 1: M(O_3P-A-(PO_3H_2)_x)_2. In chemical formula, M is a quadrivalent metal atom, A is a substituted or unsubstituted C1-20 alkylene group, substituted or unsubstituted C6-20 arylene group, substituted or unsubstituted C2-20 heteroarylene group, substituted or unsubstituted carbon cyclic group, or substituted or unsubstituted heterocyclic group; and x is a number of two or more. A fuel cell contains a composite film which includes the composite and an ion conductive polymer. The content of the composite is 1-50 parts by weight based on 100.0 parts by weight of an ion conductive polymer.
Abstract:
PURPOSE: An electrode for fuel cells, a membrane electrode assembly including the same and a fuel battery are provided to prevent outflow of phosphoric acid which is moving from a catalyst layer to a diffusion layer, thereby enhancing lifetime of the membrane electrode assembly. CONSTITUTION: An electrode for fuel cells(10) comprises a supporter(11) and a diffusion layer(13) which includes a micro pore layer(12) arranged in the supporter, a catalyst layer(14) which is arranged in order to face the micro pore layer, and a barrier layer(15) which is placed between the catalyst layer and the micro pore layer. The barrier layer has bigger average pore size than the average pore size of the micro pore layer and the catalyst layer. The membrane electrode assembly comprises a cathode, an anode which faces with the cathode, and a polymer electrolyte membrane(20) arranged between the cathode and the anode. One or more of the cathode and anode include the electrode.
Abstract:
PURPOSE: A manufacturing method of a high temperature fuel cell is provided to reduce deterioration of a fuel cell according to phosphoric acid outflow, and to reduce operation time of a fuel cell. CONSTITUTION: A manufacturing method of a high temperature fuel cell comprises: a step(S1) of raising temperature of a fuel cell stack; a step(S2) of applying a part of load to fuel cell stack, while starting the fuel cell stack when temperature of the fuel cell stack reaches to a first temperature; a step(S3) of additionally applying residual load when temperature of the fuel cell stack reaches at a second temperature.