公开(公告)号：KR100891373B1

公开(公告)日：2009-04-02

申请号：KR1020070042611

申请日：2007-05-02

Applicant: 재단법인서울대학교산학협력재단 ,  주프로스테믹스

Inventor： 김대덕 ,  강정연 ,  정정욱 ,  윤인수 ,  김선영 ,  박병순 ,  성종혁

IPC: C08B37/08

CPC classification number: C08B37/0072 ,  A61K35/30 ,  A61K35/32 ,  A61K35/34 ,  A61K35/545 ,  C08J9/28 ,  C08J2201/026 ,  C08J2201/0484 ,  C08J2205/022 ,  C08J2305/08 ,  C12N5/0068 ,  C12N2533/80 ,  C12N2537/00 ,  C12N2537/10

Abstract: Provided is a preparation method of a porous hyaluronic acid sponge comprising the steps of: dissolving hyaluronic acid in an aqueous sodium hydroxide solution; adding an epoxy-based crosslinking agent to the resultant aqueous sodium hydroxide solution in which hyaluronic acid is dissolved and homogenizing the hyaluronic acid solution; hydrogelating the homogenized hyaluronic acid solution; washing the hydrogelated hyaluronic acid hydrogel with ultrapure water; swelling the washed hyaluronic acid hydrogel to attain porosity; and freeze-drying the hyaluronic acid hydrogel to obtain a porous hyaluronic acid sponge.

公开(公告)号：KR1020070108062A

公开(公告)日：2007-11-08

申请号：KR1020070042611

申请日：2007-05-02

Applicant: 재단법인서울대학교산학협력재단 ,  주프로스테믹스

Inventor： 김대덕 ,  강정연 ,  정정욱 ,  윤인수 ,  김선영 ,  박병순 ,  성종혁

IPC: C08B37/08

CPC classification number: C08B37/0072 ,  A61K35/30 ,  A61K35/32 ,  A61K35/34 ,  A61K35/545 ,  C08J9/28 ,  C08J2201/026 ,  C08J2201/0484 ,  C08J2205/022 ,  C08J2305/08 ,  C12N5/0068 ,  C12N2533/80 ,  C12N2537/00 ,  C12N2537/10

Abstract: A method for preparing a porous hyaluronic acid sponge for a cell delivery system is provided to produce a porous hyaluronic acid sponge capable of controlling pore size thereof so as to cultivate cells well. A method for preparing a porous hyaluronic acid sponge includes the steps of: (S101) dissolving a hyaluronic acid in an aqueous sodium hydroxide solution; (S102) adding an epoxy-based crosslinking agent to the aqueous sodium hydroxide solution containing hyaluronic acid, and then homogenizing the obtained hyaluronic acid solution; (S103) hydrogelling the homogenized hyaluronic acid solution; (S104) washing the hyaluronic acid hydrogel with ultrapure water; (S105) swelling the washed hyaluronic acid hydrogel to acquire porosity; and (S106) freeze-drying the hyaluronic acid hydrogel to obtain a porous hyaluronic acid sponge.

Abstract translation: 提供了一种制备用于细胞递送系统的多孔透明质酸海绵的方法，以制备能够控制其孔径以便良好培养细胞的多孔透明质酸海绵。 制备多孔透明质酸海绵的方法包括以下步骤：（S101）将透明质酸溶解在氢氧化钠水溶液中; （S102）向含有透明质酸的氢氧化钠水溶液中添加环氧类交联剂，然后均化所得透明质酸溶液; （S103）使匀化的透明质酸溶液水凝; （S104）用超纯水洗涤透明质酸水凝胶; （S105）溶解洗涤后的透明质酸水凝胶以获得孔隙度; 和（S106）冷冻干燥透明质酸水凝胶，得到多孔透明质酸海绵。