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公开(公告)号：KR100891373B1
公开(公告)日：2009-04-02
申请号：KR1020070042611
申请日：2007-05-02
Applicant: 재단법인서울대학교산학협력재단 , 주프로스테믹스
IPC: C08B37/08
CPC classification number: C08B37/0072 , A61K35/30 , A61K35/32 , A61K35/34 , A61K35/545 , C08J9/28 , C08J2201/026 , C08J2201/0484 , C08J2205/022 , C08J2305/08 , C12N5/0068 , C12N2533/80 , C12N2537/00 , C12N2537/10
Abstract: Provided is a preparation method of a porous hyaluronic acid sponge comprising the steps of: dissolving hyaluronic acid in an aqueous sodium hydroxide solution; adding an epoxy-based crosslinking agent to the resultant aqueous sodium hydroxide solution in which hyaluronic acid is dissolved and homogenizing the hyaluronic acid solution; hydrogelating the homogenized hyaluronic acid solution; washing the hydrogelated hyaluronic acid hydrogel with ultrapure water; swelling the washed hyaluronic acid hydrogel to attain porosity; and freeze-drying the hyaluronic acid hydrogel to obtain a porous hyaluronic acid sponge.
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公开(公告)号：KR1020070108062A
公开(公告)日：2007-11-08
申请号：KR1020070042611
申请日：2007-05-02
Applicant: 재단법인서울대학교산학협력재단 , 주프로스테믹스
IPC: C08B37/08
CPC classification number: C08B37/0072 , A61K35/30 , A61K35/32 , A61K35/34 , A61K35/545 , C08J9/28 , C08J2201/026 , C08J2201/0484 , C08J2205/022 , C08J2305/08 , C12N5/0068 , C12N2533/80 , C12N2537/00 , C12N2537/10
Abstract: A method for preparing a porous hyaluronic acid sponge for a cell delivery system is provided to produce a porous hyaluronic acid sponge capable of controlling pore size thereof so as to cultivate cells well. A method for preparing a porous hyaluronic acid sponge includes the steps of: (S101) dissolving a hyaluronic acid in an aqueous sodium hydroxide solution; (S102) adding an epoxy-based crosslinking agent to the aqueous sodium hydroxide solution containing hyaluronic acid, and then homogenizing the obtained hyaluronic acid solution; (S103) hydrogelling the homogenized hyaluronic acid solution; (S104) washing the hyaluronic acid hydrogel with ultrapure water; (S105) swelling the washed hyaluronic acid hydrogel to acquire porosity; and (S106) freeze-drying the hyaluronic acid hydrogel to obtain a porous hyaluronic acid sponge.
Abstract translation: 提供了一种制备用于细胞递送系统的多孔透明质酸海绵的方法，以制备能够控制其孔径以便良好培养细胞的多孔透明质酸海绵。 制备多孔透明质酸海绵的方法包括以下步骤：（S101）将透明质酸溶解在氢氧化钠水溶液中; （S102）向含有透明质酸的氢氧化钠水溶液中添加环氧类交联剂，然后均化所得透明质酸溶液; （S103）使匀化的透明质酸溶液水凝; （S104）用超纯水洗涤透明质酸水凝胶; （S105）溶解洗涤后的透明质酸水凝胶以获得孔隙度; 和（S106）冷冻干燥透明质酸水凝胶，得到多孔透明质酸海绵。
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公开(公告)号：KR1020090111121A
公开(公告)日：2009-10-26
申请号：KR1020080036725
申请日：2008-04-21
Applicant: 주프로스테믹스
IPC: A61K35/28 , C12N5/0775 , A61K8/98
CPC classification number: A61K35/28 , A61K8/981 , C12N5/0667 , C12N2500/99
Abstract: PURPOSE: A composition for preventing or treating atopic dermatitis is provided to minimize immunological rejection by having active protein complex. CONSTITUTION: A composition for preventing or treating atopic dermatitis contains adult stem cell culture medium or its fraction as an active ingredient. The adult stem cell culture medium is supernatant, concentrate of the supernatant or freeze drive material of the supernatant. The supernatant is obtained by centrifuging the adult stem cell culture medium.
Abstract translation: 目的：提供用于预防或治疗特应性皮炎的组合物，以通过具有活性蛋白复合物来最大限度地减少免疫排斥反应。 构成：用于预防或治疗特应性皮炎的组合物含有成体干细胞培养基或其部分作为活性成分。 成体干细胞培养基是上清液，上清浓缩物或上清液的冷冻驱动材料。 通过离心成体干细胞培养基获得上清液。
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公开(公告)号：KR1020090119428A
公开(公告)日：2009-11-19
申请号：KR1020080045474
申请日：2008-05-16
Applicant: 주프로스테믹스
Abstract: PURPOSE: A pharmaceutical composition for curing scar containing culture of adult stem cell or its fraction is provided to promote division and proliferation of skin cell. CONSTITUTION: A pharmaceutical composition for curing scar contains culture of adult stem cell or its fraction as active ingredient. The culture of adult stem cell is supernatant obtained by centrifuging culture medium, concentrate or freeze-dried material of the supernatant. The culture medium of adult stem cell is obtained by sub-culturing adult stem cell in serum medium then culturing in serum free medium. The serum medium is DMEM(Dulbecco's Modified Eagle Medium) containing 1-2 mM of glutamine, 0.5-1 mM of sodium pyruvate, 0.1-10% FBS, 1% of antibiotics and 1-4.5 g/L of glucose.
Abstract translation: 目的：提供一种用于治疗含有成体干细胞培养的瘢痕或其部分的药物组合物，以促进皮肤细胞的分裂和增殖。 构成：用于治疗瘢痕的药物组合物含有成体干细胞或其部分作为活性成分的培养物。 成体干细胞的培养是通过离心上清液的培养基，浓缩物或冷冻干燥物质获得的上清液。 通过在血清培养基中培养成体干细胞，然后在无血清培养基中培养，获得成体干细胞的培养基。 血清培养基是含有1-2mM谷氨酰胺，0.5-1mM丙酮酸钠，0.1-10％FBS，1％抗生素和1-4.5g / L葡萄糖的DMEM（Dulbecco's Modified Eagle培养基）。
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公开(公告)号：KR100883565B1
公开(公告)日：2009-02-13
申请号：KR1020070015211
申请日：2007-02-14
IPC: C12N5/0775
CPC classification number: C12N5/0667 , A61K35/12 , A61K2035/124 , C12N2500/32 , C12N2500/38 , C12N2500/90 , C12N2501/115 , C12N2501/12 , C12N2501/135 , C12N2501/15 , C12N2501/165 , C12N2501/385 , C12N2501/58 , C12N2501/998
Abstract: 본 발명은 인간 지방 줄기세포의 효능을 최적화하는 성분을 함유하는 조직 재생용 주사제 첨가제에 관한 것으로서, 지방 줄기세포가 피부, 연부 조직 및 뼈 등의 미용적 또는 조직학적 결함을 교정하는 효능을 최적화하기 위하여 지방 줄기세포 활성화 화합물들을 함유하는 무혈청 배지에서 줄기세포를 1일 이상 배양하여 수득한 조건 배지를 구성성분으로 첨가한 조직 재생용 주사제 첨가제를 제공하는 것을 특징으로 한다. 본 발명에 따른 조직 재생용 주사제 첨가제는 지방 줄기세포만을 주사제로 사용하는 것에 비해 실제 투여 시 지방 줄기세포의 조직 생착률을 증대하고 조직 재생 능력을 향상시킴으로써 치료 효과를 증대하는 효과가 있다.
지방 줄기세포, 조직재생, 성장인자, 콜라겐, 주사제-
Patent Agency Ranking
公开(公告)号：KR1020080075959A
公开(公告)日：2008-08-20
申请号：KR1020070015211
申请日：2007-02-14
IPC: C12N5/0775
CPC classification number: C12N5/0667 , A61K35/12 , A61K2035/124 , C12N2500/32 , C12N2500/38 , C12N2500/90 , C12N2501/115 , C12N2501/12 , C12N2501/135 , C12N2501/15 , C12N2501/165 , C12N2501/385 , C12N2501/58 , C12N2501/998 , C12N2501/10
Abstract: An injection composition comprising culture medium for optimizing efficacy of human adipose stem cells is provided to increase tissue engraftment efficiency of adipose stem cells, improve differentiation ability of fibroblasts and enhance tissue repair ability as compared to single injection of adipose stem cells. An injection composition for tissue repair comprises cultured medium of human adipose stem cells which is prepared by culturing human adipose stem cells in serum free medium containing adipose stem cell-activating compounds including antioxidant, vitamins or their derivatives, amino acids or their derivatives, inorganic matters, and optionally growth factor and collagen for more than 1 day. The skin tissue is repaired by injecting the composition for tissue repair together with the adipose stem cell into the skin tissue. Further, the amino acids or their derivatives are one or more selected from L-Alanine, L-Asparitic acid, L-Histdine, L-leucine or L- glutamine.
Abstract translation: 提供了包含用于优化人类脂肪干细胞功效的培养基的注射组合物，以增加脂肪干细胞的组织移植效率，与单次注射脂肪干细胞相比，提高成纤维细胞的分化能力和增强组织修复能力。 用于组织修复的注射组合物包括通过在包含抗氧化剂，维生素或其衍生物，氨基酸或其衍生物的脂肪干细胞活化化合物的无血清培养基中培养人类脂肪干细胞而制备的人类脂肪干细胞的培养基，无机物 ，以及任选的生长因子和胶原蛋白超过1天。 通过将用于组织修复的组合物与脂肪干细胞一起注入皮肤组织来修复皮肤组织。 此外，氨基酸或其衍生物是选自L-丙氨酸，L-天冬氨酸，L-丝氨酸，L-亮氨酸或L-谷氨酰胺中的一种或多种。
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公开(公告)号：KR100848056B1
公开(公告)日：2008-07-23
申请号：KR1020070076221
申请日：2007-07-30
Abstract: A cosmetic composition comprising the cultured medium of adult stem cells is provided to inhibit synthesis of melanin and activity of tyrosinase without side effects, so that the composition is useful for whitening the skin. A cosmetic composition for whitening the skin comprises the cultured medium of adult stem cells derived from mammals, especially adipocytes, bone marrow tissue and umbilical cord blood of mammals or proteins separated from the cultured medium, wherein the adult stem cell is isolated from adipocytes by obtaining fat tissue through liposuction, treating the extracellular matrix of fat tissue with collagenase, centrifuging the enzyme-treated product to divide the enzyme-treated product into monocytes, hematocytes and several cell debris.
Abstract translation: 提供包含成体干细胞的培养基的化妆品组合物，以抑制黑色素的合成和酪氨酸酶的活性而没有副作用，使得该组合物可用于使皮肤美白。 用于使皮肤美白的化妆品组合物包含哺乳动物，特别是脂肪细胞，骨髓组织和来自培养基的蛋白质分离的骨髓组织和脐带血的成体干细胞的培养基，其中通过获得成体干细胞从脂肪细胞分离 通过脂肪抽吸脂肪组织，用胶原酶处理脂肪组织的细胞外基质，离心酶处理产物以将酶处理的产物分成单核细胞，血细胞和几个细胞碎片。
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公开(公告)号：KR100955212B1
公开(公告)日：2010-04-29
申请号：KR1020087019093
申请日：2006-10-12
CPC classification number: C07K14/503 , C07K14/495 , C07K14/52 , C12N5/0667 , C12N2500/02 , C12N2500/38 , C12N2500/60 , C12N2500/90 , C12N2500/99 , C12N2502/1382 , C12N2510/02
Abstract: 본 발명은 인간의 지방유래 줄기세포를 이용하여 인간의 성장인자를 대량으로 생산하는 방법에 관한 것으로서, 구체적으로 본 발명은 인간의 지방세포에서 추출된 지방유래 줄기세포를 적정한 배지 및 조건에서 배양하여 인간 성장인자를 현저히 많은 양으로 합성할 수 있도록 조작하는 방법을 제공한다. 또한 본 발명의 방법에 따라 생산된 줄기세포 배양액 및 그로부터 분리한 인간 성장인자는 약품 및 화장품의 원료로 유용하게 이용될 수 있다.
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公开(公告)号：KR100899329B1
公开(公告)日：2009-05-26
申请号：KR1020070065346
申请日：2007-06-29
Abstract: 본 발명은 인간의 중간엽 줄기세포를 이용하여 인간의 성장인자를 대량으로 생산하는 방법에 관한 것으로서, 구체적으로 본 발명은 인간의 골수, 제대혈, 태반 등에서 추출된 중간엽 줄기세포를 적정한 배지 및 조건에서 배양하여 인간 성장인자를 현저히 많은 양으로 합성할 수 있도록 조작하는 방법을 제공한다. 또한, 본 발명의 방법에 따라 생산된 줄기세포 배양액 및 그로부터 분리한 인간 성장인자는 약품 및 화장품의 원료로 유용하게 이용될 수 있다.
성장인자, 중간엽 줄기세포, 주름개선, 성장인자-
公开(公告)号：KR1020080109725A
公开(公告)日：2008-12-17
申请号：KR1020087019093
申请日：2006-10-12
CPC classification number: C07K14/503 , C07K14/495 , C07K14/52 , C12N5/0667 , C12N2500/02 , C12N2500/38 , C12N2500/60 , C12N2500/90 , C12N2500/99 , C12N2502/1382 , C12N2510/02
Abstract: A mass-production method of the growth factor by using the adipose derived stem cell is provided to improve the in vivo activity compared to the growth factor synthesized with recombinant or chemical method. The method for massively producing the human growth factor from the adipose derived stem cell comprises the steps of: (a) isolating the adipose-derived adult stem cell extracted from the adipocyte of mammals; (b) selectively culturing the stem cell in the serum medium, and subculturing it in the serum free medium; (c) adding one or more physical stimuli selected from the group consisting of low oxygen concentration cultivation, ultraviolet irradiation, nutrient deficiency and mechanical friction to the adipose derived adult stem cell; and (d) selectively adding one or more vitamins selected from vitamin A, vitamin B, vitamin C and vitamin D into the culture medium, provided that the steps (c) and (d) are performed through the combinations under the condition to occur the maximum production of human growth factor.
Abstract translation: 提供通过使用脂肪来源的干细胞的生长因子的批量生产方法，以提高与用重组或化学方法合成的生长因子相比的体内活性。 从脂肪来源的干细胞大量生产人生长因子的方法包括以下步骤：（a）分离从哺乳动物的脂肪细胞提取的脂肪来源的成体干细胞; （b）选择性培养血清培养基中的干细胞，并将其传代培养在无血清培养基中; （c）向脂肪来源的成体干细胞中加入选自低氧浓度培养，紫外线照射，营养缺乏和机械摩擦的一种或多种物理刺激物; 和（d）选择性地将一种或多种选自维生素A，维生素B，维生素C和维生素D的维生素添加到培养基中，条件是步骤（c）和（d）通过组合在条件下进行 最大生产人体生长因子。
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